Laboratory Evaluation of Toxicity of Insecticide Formulations from Different Classes against American Cockroach (Dictyoptera: Blattidae).

  • Ruhma Syed Department of Zoology, Lahore College for Women University, Lahore, Pakistan.
  • Farkhanda Manzoor Department of Zoology, Lahore College for Women University, Lahore, Pakistan.
  • Rooma Adalat Department of Zoology, Lahore College for Women University, Lahore, Pakistan.
  • Abida Abdul-Sattar Department of Zoology, Lahore College for Women University, Lahore, Pakistan.
  • Azka Syed Department of Zoology, Lahore College for Women University, Lahore, Pakistan.
Keywords: Efficacy, Insecticides, Periplaneta americana, Resistance, Topical bioassay

Abstract

Background: The present study was designed to investigate the insecticidal efficacy of four different classes of in-secticides: pyrethroids, organophosphates, phenyl-pyrazoles and neo-nicotenoids. One representative chemical from each class was selected to compare the toxicity: deltamethrin from pyrethroids, Dichlorovinyl Dimethyl Phosphate (DDVP) from organophosphates, fipronil from phenyl-pyrazoles and imidacloprid from neo-nicotenoids. The objec- tive of this study was to determine which of these insecticides were most effective against American cockroach.Methods: These insecticides were tested for their LC50 values against Periplaneta americana under topical bioassay method, using different concentrations for each chemical.Results: Fipronil 2.5% EC was highly effective at all concentrations applied, while DDVP 50% EC was least toxic amongst all. One way analysis of variance confirmed significant differences between mortality of P. americana and different concentrations applied (P< 0.05).Conclusion: Locality differentiation is an important factor in determining the range of resistance between various localities, as all three localities behaved differently in terms of their levels of resistance.

References

Abd-Elghafar SF, Appel AG, Mack TP (1990) Toxicity of several insecticide formula- tions against adult German cockroach- es (Dictyoptera: Blattellidae). J Econ Entomol. 83: 2290–2294.

Ahmad I, Suliyat A (2011) Development Of Fipronil Gel Bait Against German Cock- roaches, Blattella germanica (Dictyop- tera: Blattellidae). J Entomol. 8(3):288–294.

Ahmad I, Sriwahjuningsih SA, Putra RE, Permana AD (2009) Monitoring pyre- throid resistance in field collected Blattella germanica Linn. (Dictyoptera: Blattellidae) in Indonesia. Entomol Res.39: 114–118.

Ahmed AS, Bashir NH, Assad YO (2010) Susceptibility of Periplaneta americana (L.) (Orthoptera: Blattidae) population from wad medani (Sudan Gezira) to three public health insecticides. Rest Pest Manag Newsletter. 19(2): 8–14.

Appel AG, Tanley MJ (2000) Laboratory and field performance of imidacloprid gel bait against german cockroaches (Dictyoptera: Blattellidae) in Indone- sia. Entomol Res. 39: 114–118.

Appel AG, Reierson DA, Rust MK (1983) Comparative water relations and tem- perature sensitivity of cockroaches. Comp Biochem Physiol. 74: 357–361.

Ballard JB, Gold RE, Rauscher JD (1984) Effectiveness of six insecticide treat- ment strategies in the reduction of German cockroach (Orthoptera: Blattellidae) populations in infested apartments. J Econ Entomol. 77: 1092–1094.

Barcay SJ (2004) Cockroaches. In: Hand- book of pest control (ed. by Mallis, A)9th ed. GIE Media, Cleveland, Ohio, United States. pp. 121–215.

Chai RY, Lee CY (2010) Insecticide Re- sistance Profiles and Synergism in Field Populations of the German Cockroach (Dictyoptera: Blattellidae) From Singa- pore. J Econ Entomol. 103(2): 460–471.

Choo LE, Tang CS, Pang FY, Ho SH (2000) Comparison of two bioassay methods for determining deltamethrin resistance in German cockroaches (Blattodea: Bla- ttellidae). J Econ Entomol. 93: 905–910.

Cole LM, Nicholson RA, Casida JE (1993) Action of Phenyl pyrazole Insecticides at the GABA-Gated Chloride Channel. Pestic Biochem Physiol. 46: 47–54.

Czajka E, Pancer K, Kochman M, Gliniewiez A, Sawicka B, Rabczenko D (2003) Characteristics of bacteria isolated from body surface of German cockroaches caught in hospitals. Przegl Epidemiol.57(4): 655–662.

Fasulo TR, Kern WH, Koehler PG, Short DE (2005) Pests In and Around the Home. University of Florida, United States, pp. 124–126.

Gadd CA and Raubenheimer D (2000) Nu- trient specific learning in an omniv - orous insect: the American cockroach Periplaneta americana L. learns to as- sociate dietary protein with the odors citral and carvone. J Insect Behav. 13:851–864.

Gore JC, Schal C (2007) Cockroach Aller- gen Biology and Mitigation in the In door Environment. Annu Rev Entomol.52: 439–463.

Holbrook G, Roebuck J, Moore C, Waldvogel M, Schal C (2003) Origin and extent of resistance to fipronil in the german cockroach, Blattella germanica (L.)(Dictyoptera: Blattellidae). J Econ Entomol. 96(5): 1548–1558.

Kinfu A, Erko B (2008) Cockroaches as car- riers of human intestinal parasites in two localities in Ethiopica. Trans R Soc Trop Med Hyg. 102: 1143–1147.

Kaakeh W, Reid BL, Bennett GW (1997) Toxicity of fipronil to German and American cockroaches. Entomol Exp Appl. 84: 229–237.

Lane HC, Montagne JL, Fauci AS (2001) Bioterrorism: A clear and present dan- ger. Nature Medicine. 7: 1271–1273.

Lee CY (1998) Control of insecticide-resistant German cockroaches, Blattella germa- nica (L.) (Dictyoptera: Blattellidae) in food-outlets with hydramethylnon- based bait stations. Trop Biomed. 15: 45–51.

Lee CY, Lee LC (2000) Influence of sani- tary condition on the field performance of chlorpyrifos based baits against American cockroaches, Periplaneta americana (L.) (Dictyoptera: Blattidae). J Vect Ecol. 25: 218–221.

Lee CY, Robinson W (2001) Handbook of Malaysian Household and Structural Pests. Pest Control Association of Ma- laysia, Kuala Lumpur, Malaysia, pp.96–108.

Lee CY, Hemingway J, Yap HH, Chong NL (2000) Biochemical characterization of insecticide resistance in the German cockroach, Blattella germanica, from Malaysia. Med Vet Entomol. 14: 11–18.

Lee CY, Yap HH, Chong NL (1997) Elevated esterase detection in insecticide resistant German cockroaches (Dictyoptera: Blattellidae) using modified Pasteur- Georghiou's filter-paper method. Trap Biomed. 14: 81–86.

Lee CY, Yap HH, Chong NL, Lee RS (1996) Insecticide resistance and synergism in field-collected German cockroaches (Dictyoptera: Blattellidae) in Peninsular Malaysia. Bull Entomol Res. 86: 675–682.

Lee KM, Lee CY (2002) Prevalence of in- secticide resistance in field-collected populations of the German cockroach, Blattella germanica (Linnaeus) (Dictyop- tera: Blattellidae) in Peninsular Malay- sia. Med Entomol. 53(4): 219–225.

Lee LC, Lee CY (2004) Insecticide re- sistance profiles and possible under- lying mechanisms in German cock- roaches, Blattella germanica (Linnaeus) (Dictyoptera: Blattellidae) from Pen- insular Malaysia. Med Entomol Zool.55(2): 77–93.

Morane G (2002) Threats in bioterrorism II: CDC category B and C agents. Emerg Med Clin N Am. 20: 311–330.

Mpuchane S, Matsheka IM, Gashe BA, Allotey J, Murindamombe, G, Mrema N (2006) Microbiological studies of cock- roaches from three localities in Gabo- rone, Botswana. Afr J Food Nutr Sci.6: 56–59.

Nasirian H, Ladonni H, Vatandoost H (2006) Duration of Fipronil Topical Applica- tion Toxicity in Blattella germanica Field Population Strains. Pak J Biol Sci. 9(5): 800–804.

Nasirian H, Ladonni H, Vatandoost H (2009) Duration of Fipronil Topical Applica- tion Toxicity in Blattella germanica Field Population Strains. Pak J Biol Sci. 9(5):800–804.

Nasirian H, Ladonni H, Shayeghi M, Vatan- doost H, Yaghoobi MR, Rassi Y, Abol- hassani M, Abaei MR (2006) Compar- ison of Permethrin and Fipronil Tox- icity against German cockroach (Dic- tyoptera: Blattellidae) Strains. Iranian J Publ Health. 35(1): 63–67.

Pechal JL, Austin J, Gold R, Tomberlin JK (2007) Epidemiology and Spatial Re- lationships of Bacteria Associated with Periplaneta americana (L.) (Blattodea: Blattidae) in Central Texas. J Agric Ur- ban Entomol. 24(4): 205–216.

Rettich F, Stejskal V (2008) Residual effect of Fipronil SC against insecticide re- sistant populations of the german cock- roach. Proceedings of the 6th Interna- tional Conference on Urban Pests (ed. by Robinson WH and Bajomi D), pp.501–504.

Rina T, Tilak VW, Yadav JD, Dutta G (2002) Efficacy of fipronil and propoxur in the control of German cockroaches (Dictyoptera: Blattellidae). J Com Dis.34: 65–69.

Salgado VL (1997) The modes of action of spinosad and other insect control prod- ucts. Down Earth. 52: 35–43.

Sarinho ED, Schor MA, Rizzo JA (2004) There are more asmatics in homes with high cockroach infestation. Braz J Biol Res. 37: 503–510.

Scharf ME, Hemingway J, Reid BI, Small GJ, Bennett GW (1996) Toxicological and biochemical characterization of in- secticide resistance in a field collected strain of Blattella germanica (Dic- tyoptera: Blattellidae). J Econ Entomol.89: 322–331.

Scharf ME, Kaakeh W, Bennett GW (1997) Changes in an insecticide resistant field-population of German cockroach following exposure to an insecticide mixture. J Econ Entomol. 90: 38–48.

Shafiqur-Rahman AS, Akter MY (2006) Toxicity of diazinon and cypermethrin against the American cockroach, Peri- planeta americana (L.) Univ. J Zool Rajshahi. 25: 63–64.

Sulaiman S, Muhammad AH, Othman H (2007) Efficacy of hydramethylnon and fipronil get baits with laboratory and field strains of Periplaneta ameri- cana (L.) (Dictyoptera: Blattidae) in Malaysia. J Trop Med Parasitol. 30:64–67.

Tsai C, Lee H (2001) Analysis of specific adaptation to a domicile habitat: A comparative study of two closely related cockroach species. J Med Entomol.38: 245–252.

Valles SM, Koehler PG, Brenner RJ (1997) Antagonism of fipronil toxicity by piperonyl butoxide and S, S, S,-tributyl phosphorotrithioate in the German cockroach (Dictyoptera: Blattellidae). J Econ Entomol. 90: 1254–1258.

Vythilingam I, Jeffery J, Oothuman P, Abdul Razak AR, Sulaiman A (1997) Cock- roaches from human dwellings: isola- tion of bacterial pathogens and control. Southeast Asian J Trop Med Pub Hlth.28: 218–222.

Wang C, Scharf M, Bennett G (2004) Be- havioral and physiological resistance of the German cockraoch to gel bait(Blattodea: Blattellidae). J Econ En- tomol. 97(6): 2067–2072.

Watanabe H, Kobayashi Y, Sakura M, Matsu- moto Y, Mizunami M (2003) Classical olfactory conditioning in the cockroach Periplaneta americana. Zool Sci. 20:1447–1454.

Wei Y, Appel AG, Moar WJ, Liu N (2001) Pyrethroid resistance and cross-resistance in the German cockroach, Blatella ger- minca (L). Pest Manag Sci. 57: 1055–1059.

Zhai J, Robinson WH (1996) Instability of cypermethrin resistance in a field pop- ulation of the German cockroach (Or- thoptera: Blattellidae). J Econ Entomol.89: 332–336.

How to Cite
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Syed R, Manzoor F, Adalat R, Abdul-Sattar A, Syed A. Laboratory Evaluation of Toxicity of Insecticide Formulations from Different Classes against American Cockroach (Dictyoptera: Blattidae). J Arthropod Borne Dis. 8(1):21-34.
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