Molecular Identification of Leishmania Species in a Re-Emerged Focus of Cutaneous Leishmaniasis in Varamin District, Iran
AbstractBackground: Cutaneous leishmaniasis (CL) is one of the most important neglected tropical diseases and a major public health challenge in Iran caused by Leishmania spp and transmitted by phlebotomine sand flies. The number of CL cases has shown an increasing pattern all over the country, including the district of Varamin, southeast of Tehran, Iran. This study aimed to identify the Leishmania spp isolated from CL patients using molecular methods in Varamin during 2012–2013.Methods: Exudate materials collected from the swollen edge of the skin lesions of 44 parasitological positive CL patients by disposable lancet. They were referred to Varamin Health Center by physician. The samples were subjected to molecular method for Leishmania species identification.Results: The digestion pattern of restriction enzyme revealed that 37 (84.1%) CL patients were infected with L. major and 7 (15.9%) were infected with L. tropica. They were mostly male than female. More than half of the patients (58%) had multiple lesions, and they were mostly observed on extremities, 34.1% on legs and 29.5% on hands. Lesions were mostly of wet ulcerative type.Conclusion: Dominancy of L. major provides more evidence that Varamin District probably could be considered as Zoonotic Cutaneous Leishmaniasis (ZCL) areas. More investigation on other epidemiological aspects of disease is needed.
Akhavan AA, Yaghoobi-Ershadi MR, Khame- sipour A, Mirhendi H, Alimohammadian MH, Rassi Y, Arandian MH, Jafari R, Abdoli H, Shareghi N, Ghanei M, Jalali-zand N (2010) Dynamics of Leish- mania infection rates in Rhombomys opimus (Rodentia: Gerbillinae) popu- lation of an endemic focus of zoonotic cutaneous leishmaniasis in Iran. Bull Soc Pathol Exot. 103: 84–89.
Al-Nahhas SA, Kaldas RM (2013) Charac- terization of Leishmania species iso- lated from cutaneous human samples from central region of Syria by RFLP analysis. ISRN Parasitol. 3: 5–11.
Alvar J, Velez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M (2012) Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 7:e35671.
Ardahali S, Rezaei HR, Nadim A (1994) Leishmania and leishmaniases. 2nd ed. Markaze Nashr Daneshgah Press, Tehran.
Azizi K, Moemenbellah-Fard MD, Kalantari M, Fakoorziba MR (2012) Molecular detection of Leishmania major kDNA from wild rodents in a new focus of zoonotic cutaneous leishmaniasis in an oriental region of Iran. Vector Born Zoontic Dis. 12(10): 844–850.
Behravan MR, Hajjaran H, Abadi A, Haghi- ghi A, Rahbarian N, Amini A , Hoo- bar MR, Khajeh- Farokhi M, Moin- Vaziri V (2015) Cutaneous leishman- iasis in suspected refereed patients to health centers of Varamin and deter- mination of sand flies species during
–2013. Med J Tabriz Uni Med Sci
Health Services. 37(2): 6–11. Fakoorziba MR, Baseri A, Eghbal F, Rezaee
S, Azizi K, Moemenbellah-Fard MD (2011) Post-earthquake outbreak of cu- taneous leishmaniasis in a rural region of southern Iran. Ann Trop Med Parasi-tol. 105(3): 217–224.
Hajjaran H, Mohebali M, Teimouri A, Oshaghi MA, Mirjalali H, Kazemi-Rad, Shiee MR, Naddaf SR (2014) Identification and phylogenetic relationship of Ira- nian strains of various Leishmania spe- cies isolated from cutaneous and vi- sceral cases of leishmaniasis based on N-acetylglucosamine-1-phosphate trans- ferase gene. Infect Genet Evol. 26: 203–212.
Hajjaran H, Vasigheh F, Mohebali M, Rezaei S, Mamishi S, Charedar S (2011) Di- rect diagnosis of Leishmania species on serosity materials punctured from cuta- neous leishmaniasis patients using PCR- RFLP. J Clin Lab Anal. 25: 20–24.
Hajjaran H, Mohebali M, Mamishi S, Vasigheh F, Naddaf SR, Teimouri A, Edrissian GH, Zarei Z (2013) Molecular identi- fication and polymorphism determina- tion of cutaneous and visceral leish- maniasis agents isolated from human and animal hosts in Iran. BioMed Res Int. pp. 1–7.
Khosravi A, Sharifi I, Dortaj E, Aghaei Afshar A, Mostafavi M (2013) The present status of cutaneous leishmaniasis in a recently emerged focus in south- west of Kerman Province, Iran. Iran J Public Health. 42: 182–187.
McDowell MA, Rafati S, Ramalho-Ortigao M, Salah AB (2011) Leishmaniasis: Middle East and North Africa research and development priorities. PLoS Negl Trop Dis. 5:e1219.
Nateghi Rostami M, Saghafipour A, Vesali E (2013) A newly emerged cutaneous leishmaniasis focus in central Iran. Int J of Inf Dis. 17. e1198–e1206.
Parvizi P, Moradi G, Akbari G, Farahmand M, Ready P, Piazak N, Assmar M, Amirkhani A (2008) PCR detection and sequencing of parasite ITS-rDNA gene from reservoirs host of zoonotic cuta- neous leishmaniasis in central Iran. Parasitol Res. 103: 1273–1278.
Parvizi P, Ready P (2008) Nested PCRs and sequencing of nuclear ITS-rDNA frag- ments detect three Leishmania species of gerbils in sandflies from Iranian foci of zoonotic cutaneous leishmaniasis. Trop Med Int Health. 13: 1159–1171.
Rafati N, Shaporimoghadem A, Ghorbani R (2007) Epidemiological study of cuta- neous leishmaniasis in Damghan (2000–2006). J Semnan Univ Med Sci. 24:250–251.
Reithinger R, Dujardin J-C, Louzir H, Pirmez C, Alexander B, Brooker S (2007) Cutaneous leishmaniasis. Lancet Infect Dis 7: 581–596.
Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, Jaffe CL (2003) PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis. 47: 349–358.
Sharifi I, Zamani F, Aflatoonian MR, Fekri AR (2008) An epidemic of cutaneous leishmaniasis in Baft District in Kerman Province and its probable causative risk factors. Iran J Epidemiol. 4: 53–8.
Shirzadi MR, Esfahani B, Mohebali M, Yaghoobi Ershadi MR, Gharachorlo F, Razavi MR, Postigo JAR (2015) Epide-miological status of leishmaniasis in the Islamic Republic of Iran, 1983–2012. East Mediterr Health J. 21(10):736–742.
Talari SA, Talaei R, Shajari G, Vakili Z, Taghaviardakani A (2006) Childhood cutaneous leishmaniasis: report of 117 cases from Iran. Korean J Parasitol.44: 355–60.
WHO (1991) Basic laboratory methods in medical parasitology. Available at: www.who.int/malaria/publications/ato z/9241544104_part1/en.
Yaghoobi-Ershadi M, Akhavan A, Zahraei- Ramazani A, Abai M, Ebrahimi B, Vafaei-Nezhad R, Hanafi-Bojd AA, Jafari R (2003) Epidemiological study in a new focus of cutaneous leishma- niasis in the Islamic Republic of Iran. East Mediterr Health J. 9: 816–826.
Yaghoobi-Ershadi MR, Hakimiparizi M, Zahraei-Ramazani AR, Abdoli H, Akhavan AA, Aghasi M, Arandian M, Ranjbar A (2010) Sand fly surveillance within an emerging epidemic focus of cutaneous leishmaniasis in southeast- ern Iran. Iran J Arthropod Borne Dis.4(1): 17–23.
Yaghoobi-Ershadi MR (2012) Phlebotomine sand flies (Diptera: Psychodidae) in Iran and their role on Leishmania transmi- ssion. J Arthropod Borne Dis. 6(1): 1–17.