Molecular Typing and Phylogenetic Analysis of Some Species Belonging to Phlebotomus (Larroussius) and Phlebotomus (Adlerius) Subgenera (Diptera: Psychodidae) from Two Locations in Iran

  • P Parvizi Molecular Systematics Laboratory, Pasteur Institute of Iran, Tehran, Iran
  • SR Naddaf Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
  • E AlaeeNovin Molecular Systematics Laboratory, Pasteur Institute of Iran, Tehran, Iran
Keywords: Mitochondrial Cytochrome b, Phlebotomus (Larroussius) major, Phlebotomus (Larroussius) tobbi, Phlebotomus (Adlerius) halepensis, Iran,

Abstract

Background: Haematophagous females of some phlebotomine sandflies are the only natural vectors of Leishmania species, the causative agents of leishmaniasis in many parts of the tropics and subtropics, including Iran.  We report the presence of Phlebotomus (Larroussius) major and Phlebotomus (Adlerius) halepensis in Tonekabon (Ma­zanderan Province) and Phlebotomus (Larroussius) tobbi in Pakdasht (Tehran Province). It is the first report of these species, known as potential vectors of zoonotic visceral leishmaniasis in Iran, are identified in these areas.Methods: In 2006-2007 individual wild-caught sandflies were characterized by both morphological features and sequence analysis of their mitochondrial genes (Cytochrome b).  The analyses were based on a fragment of  494 bp at the 3´ end of the Cyt b gene (Cyt b 3´ fragment) and a fragment of  382 bp CB3 at the 5´ end of the Cyt b gene (Cyt b 5´ fragment). We also analysed the Cyt b Long fragment, which is located on the last 717 bp of the Cyt b gene, followed by 20 bp of intergenic spacer and the transfer RNA ser(TCN) gene.Results: Twenty-seven P. halepensis and four P. major from Dohezar, Tonekabon, Mazanderan province and 8 P. tobbi from Packdasht, Tehran Province were identified by morphological and molecular characters. Cyt b 5´ and Cyt b 3´ fragment sequences were obtained from 15 and 9 flies, respectively. Cyt b long fragment sequences were ob­tained from 8 out of 27 P. halepensis.Conclusion: Parsimony analyses (using heuristic searches) of the DNA sequences of Cyt b always showed mono­phyletic clades of subgenera and each species did form a monophyletic group.

References

Aransay AM, Scoulica E, Chaniotis B, Tselen- tis Y (1999) Typing of sandflies from Greece and Cyprus by DNA polymor- phism of 18S rRNA gene. Insect Mol Biol. 8: 179–184.

Azizi K, Rassi Y, Javadian E, Motazedian MH, Rafizadeh S, Yaghoobi Ershadi MR, Mohebali M (2006) Phlebotomus (Paraphlebotomus) alexandri: a probable vector of Leishmania infantum in Iran. Ann Trop Med Parasitol. 100(1): 63–8.

Depaquit J, Ferte H, Leger N, Killik-Ken- drick R, Rioux JA, Killick-Kendrick M, Hanafi HA, Gobert S (2000) Mo- lecular systematics of the Phlebotomine sandflies of the subgenus Paraphleboto- mus (Diptera, Psychodidae, Phleboto- mus) based on ITS2 rDNA sequences. Hypotheses of dispersion and speci- ation. Insect Mol Biol. 9: 293–300.

Esseghir S, Ready PD, Ben-Ismail R (2000) Speciation of Phlebotomus sandflies of the subgenus Larroussius coincided with the late Miocene-Pliocene aridification of the Mediterranean subregion. Biol J Linn Soc. 70: 189–219.

Esseghir S, Ready PD, Killick-Kendrick R, Ben-Ismail R (1997) Mitochondrial hap- lotypes and phylogeography of Phle- botomus vectors of Leishmania major. Insect Mol Biol. 6: 211–225.

Killick-Kendrick R (1990) Phlebotomine vec- tors of the leishmaniases: A review. Med Vet Entomol. 4: 1–24.

Killick-Kendrick R (1999) The biology and control of phlebotomine sandflies. Clin Derm. 17: 279–289.

Lewis DJ (1982) A taxonomic review of the genus Phlebotomus (Diptera: Psychodi- dae). Bull Brit Mus Nat Hist Ent. 45:121–209.

Mohebali M, Hajjaran H, Hamzavi Y, Mo- bedi I, Arshi S, Zarei Z, Akhoundi B, Naeini EKM, Avizeh R, Fakhar M (2005) Epidemiological aspects of canine visceral leishmaniasis in the Islamic Re- public of Iran. Vet Parasitol. 129: 243–251.

Muccio TD, Marinucci M, Frusteri L, Maroli M, Pesson B, Gramiccia M (2000) Phylogenetic analysis of Phlebotomus species belonging to the subgenus Lar- roussius (Diptera, Psychodidae) by ITS2 rDNA sequences. Insect Biochem Mol Biol. 30: 387–393.

Nadim A, Javadian E (1976) Key for species identification of sandflies (Phlebotomi- nae; Diptera) of Iran. Iranian J Publ Hlth.5: 35-44.

Nadim A, A Navid-Hamidi, E Javadian, GH Tahvildari Bidruni and H Amini (1978) Present status of Kala-azar in Iran. Am J Trop Med Hyg. 27: 25–28.

Nadim A, Javidian E, Tahvildar-Bidrun IG, Mottaghi M, Abai MR (1992) Epide- miological aspects of kala-azar in Mesh- kin-Shahr, Iran: investigation on vectors. Iran.

Parvizi P, Mazloumi Gavgani AS, Davies CR, Courtenay O, Ready PD (2008) Two Leishmania species circulating in the Ka- leybar focus of ‘infantile visceral leishma- niasis’, northwest Iran: implications for deltamethrin dog collar intervention. Trans R Soc Trop Med Hyg. 102: 891–897.

Parvizi P, Amirkhani A (2008) Mitochon- drial DNA characterization of popula- tions of Sergentomyia sintoni and find- ing mammalian Leishmania infections in this sandfly by using ITS-rDNA gene. Iran J Vet Res. 9: 9–18.

Parvizi P, Ready PD (2006) Molecular inves- tigation of the population differentiation of Phlebotomus papatasi, important vector of Leishmania major in differ- ent habitats and regions of Iran. Iran Biomed J. 10: 69–77.

Parvizi P, Benlarbi M, Ready PD (2003) Mitochondrial and Wolbachia markers for the sandfly Phlebotomus papatasi: little population differentiation between peridomestic sites and gerbil burrows in Isfahan province, Iran. Med Vet Ento- mol. 17: 351–362.

Sudia WD, Chamberland RW (1962) Battery operated light trap, an improved model. Mosquito News. 22: 126–129.

Swofford DL (2002) PAUP: Phylogenetic Analysis Using Parsimony (and other methods) version 4.0. Sinauer Associates, Sunderland, Massachusetts.

How to Cite
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Parvizi P, Naddaf S, AlaeeNovin E. Molecular Typing and Phylogenetic Analysis of Some Species Belonging to Phlebotomus (Larroussius) and Phlebotomus (Adlerius) Subgenera (Diptera: Psychodidae) from Two Locations in Iran. J Arthropod Borne Dis. 4(2):1-10.
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