Histopathological Study of Esophageal Infection with Gasterophilus pecorum (Diptera: Oestridae) in Persian Onager (Equus hemionus onager)

  • Seyed Mohammad Hoseini Department of Pathobiology, Babol Branch, Islamic Azad University, Babol, Iran
  • Bahram Ali Zaheri Department of Environmental Protection Semnan, Semnan, Iran
  • Mohamad Ali Adibi Department of Environmental Protection Semnan, Semnan, Iran
  • Hooman Ronaghi Department of Microbiology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • Amir Hossein Moshrefi Young Researchers and Elite Club, Babol Branch, Islamic Azad University, Babol, Iran
Keywords: Persian onager, Gasterophilus pecorum, Epithelial destruction

Abstract

Background: The larval stages of Gasterophilus are obligate parasites in the gastrointestinal tract of equine account­able for pathologic ulcers in the Persian onager gastrointestinal. The aim of the current report was to study the histo­pathological change with G. pecorum larvae in the esophagus of a Persian onager.Methods: This study was performed in Iranian Zebra propagation and breeding site in Khartouran National Park, southeast of Shahrud City, Semnan Province, Iran in 2014. Following a necropsy with specific refer to esophagus of one adult female Persian onager were transmitted to the laboratory. After autopsy, parasites collected from the esophagus were transmitted into 70% alcohol. For histopathological investigation, tissue samples were collected from the esophagus. The tissues were fixed in 10% buffered formalin, and conformity routine processing, there were stained with Hematoxylin and eosin.Results: After clarity by lactophenol parasites were identified as G. pecorum. Microscopic recognition contained hyperemia, inflammatory cell infiltration, epithelial destruction, esophageal gland hyperplasia.Conclusion: This is the first survey of G. pecorum and histopathological study in the Persian onager esophagus in the world.

References

Tatin L, Darreh-Shoori BF, Tourenq C, Tatin D, Azmayesh B (2003) The last populations of the Critically Endangered onager Equus hemionus onager in Iran: urgent requirements for protection and study. Oryx. 37(4): 488–491.

Gökçen A, Sevgili M, Altaş MG, Camk-erten İ (2008) Presence of Gasterophi-lus species in Arabian horses in San-liurfa region. Turkiye Parazitol Derg. 32: 337–339.

Liu SH, Hu DF, Li K (2015) Oviposition site selection by Gasterophilus pecorum (Diptera: Gasterophilidae) in its habitat in Kalamaili Nature Reserve, Xinjiang, China. Parasite. 22: 34.

Studzińska MB, Wojcieszak K (2009) Gas-terophilus sp. botfly larvae in horses from the south-eastern part of Poland. Bull Vet Inst Pulawy. 53: 651–655.

Ganjali M, Keighobadi M (2016) A Rare Case of Gastric Myiasis in a Lion Caused by Gasterophilus intestinalis (Diptera: Gasterophilidae)-Case Report. J Arthro- pod Borne Dis. 10(3): 423–425.

Otranto D, Milillo P, Capelli G, Colwell DD (2005) Species composition of Gaster-ophilus spp. (Diptera, Oestridae) causing equine gastric myiasis in southern Italy: parasite biodiversity and risks for extinc-tion. Vet Parasitol. 133(1): 111–118.

Mashayekhi M, Ashtari B (2013) Study of Gasterophillus role in equine gastric ulcer syndrome in Tabriz area. Bull Env Pharmacol Life Sci. 2(12): 169–172.

Zumpt F (1965) Myiasis in man and ani-mals in the Old World. A textbook for physicians, veterinarians and zoologists, Butterworths, London.

Sharir B, Pipano E, Markovics A, Danieli Y (1987) Field studies on gastrointesti-nal infestation in Israeli Horses. Isr J Vet Med. 43: 223–227.

Hoglund J, Ljungstrom BL, Nilsson O, Lundguist H, Osterman E, Uggla A (1997) Occurrence of Gasterophilus in-testinalis and some parasitic nematodes of horses in Sweden. Acta Vet Scand. 38: 157–165.

Sweeney HJ (1990) The prevalence and pathogenicity of Gasterophilus intesti-nalis larvae in horses in Ireland. Irish Vet J. 43: 67–73.

Bernard N, Collobert C, Tariel G, Lamidey C (1994) Epidemiological survey of bot infection in horses at necropsy in Normandy from April 1990 to March 1992. Rec Med Vet. 170: 231–235.

Edwards GT (1982) The prevalence of Gasterophilus intestinalis in horses in northern England and Wales. Vet Para-sitol, 11: 215–222.

Agneessens J, Engelen S, Debever P, Vercruysse J (1998) Gasterophilus in-testinalis infections in horses in Bel-gium. Vet Parasitol. 77: 199–204.

Brocard P, Pfister K (1991) The epidemi-ology of gasterophilosis of horses in Switzerland. Schweiz Arch Tierheilkd. 133: 409–416.

Drudge JH, Lyons ET, Wyant ZN, Tol-liver SC (1975) Occurrence of second and third instars of Gasterophilus intes-tinalis and Gasterophilus nasalis in stomachs of horses in Kentucky. Am J Vet Res. 36: 1585–1588.

Pandey VS, Ouhelli H, Elkhalfane A (1980) Observations on the epizootiol-ogy of Gasterophilus intestinalis and G. nasalis in horse in Morocco. Vet Para-sitol. 7: 347–356.

Tavassoli M, Bakht M (2012) Gastrophi-lus spp. myiasis in Iranian equine. Sci Parasitol. 13(2): 83–86.

Liu SH, Hu DF, Li K (2016a) Parasites observed in the proximal alimentary tract of a Przewalski's horse in China. Equine Veterinary Education. Availa-ble at: http://onlinelibrary.wiley.com/doi/ 10.1111/eve.12593/full

Liu SH, Li K, Hu DF (2016b) The inci-dence and species composition of Gas-terophilus (Diptera, Gasterophilidae) caus-ing equine myiasis in northern Xin-jiang, China. Vet Parasitol. 217: 36–38.

Zaheri BA, Ronaghi H, Youssefi MR, Hoseini SM, Omidzahir S, Dozouri R, Eshkevari SR, Mousapour A (2015) Gas-terophilus pecorum and Habronema mus-cae in Persian onager (Equus hemionus onager), histopathology and parasitology survey. Comp Clin Path. 24(5): 1009–1013.

Published
2017-10-08
How to Cite
1.
Hoseini SM, Zaheri BA, Adibi MA, Ronaghi H, Moshrefi AH. Histopathological Study of Esophageal Infection with Gasterophilus pecorum (Diptera: Oestridae) in Persian Onager (Equus hemionus onager). J Arthropod Borne Dis. 11(3):441-445.
Section
Case Report