Effects of Diatomaceous Earth on House Dust Mite Sensitization in BALB/c Mice

  • Sung-Ying Liu Renai Biotechnology Limited, Taichung, Taiwan
  • Yu-Hui Chang Green Polymer Corporation, Taichung, Taiwan
  • Hui-Ru Ji Graduate Institute of Biomedical Science, China Medical University, Taichung, Taiwan
  • Cheng-Di Chiu Graduate Institute of Biomedical Science, China Medical University, Taichung, Taiwan AND School of Medicine, China Medical University, Taichung, Taiwan AND Neurosurgical Department, China Medical University Hospital, Taichung, Taiwan
Keywords: Asthma, Diatomaceous earth, House dust mite, Allergy, Environmental exposure

Abstract

Background: House dust mite (HDM) is associated with hypersensitivity such as asthma. It is suggested that patients with asthma benefit from improved living environment by reducing HDM exposure. In this study, we examined the effects of commercialized diatomaceous earth product, Casaggia® used as construction materials, on hypersensitivity in HDM-sensitized mice. Methods: Male mice were sensitized with house dust mite (HDM) extract for 7 days and then housed in diatomaceous earth (DE)-coated cages for 14 days. Levels of cytokine were determined using ELISA. White blood cell counts were recorded over 21 days. Histological analysis was conducted to determine the remodeling of respiratory tract. Results: The data showed that exposure to diatomaceous earth (DE) resulted in a suppression in elevated eosinophilia induced by HDM in mice. In addition, elevated serum IgE responding to HDM sensitization were restored in the presence of DE. The result revealed that DE ameliorated the inflammation progression in airway. Conclusion: Environmental exposure to DE is suggested to benefit patients with hypersensitivity through relieving inflammatory symptoms. In a sense of prevention, DE represents a potential material against development of asthma.  

References

Yangzong Y, Shi Z, Nafstad P, Haheim LL, Luobu O, Bjertness E (2012) The prevalence of childhood asthma in China: a systematic review. BMC Public Health. 12: 860.

Greenlee RT, Chyou PH, Kieke A, Naik YG, Kirkhorn S (2008) Prevalence of asthma in a general population cohort of farm children: comparison of estimates based on parental report and medical record review. J Agromedicine. 13(4): 225–231.

de Vries MP, van den Bemt L, Thoonen BP, Muris JW, van Schayck CP (2006) Re-lationship between house dust mite (HDM) allergen exposure level and inhaled corticosteroid dosage in HDM-sensitive asthma patients on a self management program. Prim Care Respir J. 15(2): 110–115.

de Vries MP, van den Bemt L, van der Mooren FM, Muris JW, van Schayck CP (2005) The prevalence of house dust mite (HDM) allergy and the use of HDM-impermeable bed covers in a primary care population of patients with persis¬tent asthma in the Netherlands. Prim Care Respir J. 14(4): 210–214.

Wang JY (2013) The innate immune response in house dust mite-induced allergic inflammation. Allergy Asthma Immunol Res. 5(2): 68–74.

Geden CJ, Devine GJ (2012) Pyriproxyfen and house flies (Diptera: Muscidae): ef¬fects of direct exposure and autodissemination to larval habitats. J Med Ento¬mol. 49(3): 606–613.

Iatrou SA, Kavallieratos NG, Palyvos NE, Buchelos CT, Tomanovic S (2010) Acaricidal effect of different diatomaceous earth formulations against Tyrophagus putrescentiae (Astigmata: Acaridae) on stored wheat. J Econ Entomol. 103(1): 190–196.

Athanassiou CG, Kavallieratos NG, Peteinatos GG, Petrou SE, Boukouvala MC, Tomanovic Z (2007) Influence of temperature and humidity on insecticidal effect of three diatomaceous earth formulations against larger grain borer (Coleoptera: Bostrychidae). J Econ Entomol. 100(2): 599–603.

Denli M, Blandon JC, Guynot ME, Salado S, Perez JF (2015) Efficacy of activated diatomaceous clay in reducing the toxicity of zearalenone in rats and piglets. J Anim Sci. 93(2): 637–645.

Martin CD, Mullens BA (2012) Housing and dustbathing effects on northern fowl mites (Ornithonyssus sylviarum) and chicken body lice (Menacanthus stramineus) on hens. Med Vet Entomol. 26 (3): 323–333.

Fattouh R, Al-Garawi A, Fattouh M, Arias K, Walker TD, Goncharova S, Coyle AJ, Humbles AA, Jordana M (2011) Eosinophils are dispensable for allergic remodeling and immunity in a model of house dust mite-induced airway disease. Am J Respir Crit Care Med. 183(2): 179–188.

Phillips JE, Peng R, Harris P, Burns L, Renteria L, Lundblad LK, Fine JS, Bauer CM, Stevenson CS (2013) House dust mite models: will they translate clini-cal¬ly as a superior model of asthma? J Allergy Clin Immunol. 132(1): 242–244.

Matsuoka H, Maki N, Yoshida S, Arai M, Wang J, Oikawa Y, Ikeda T, Hirota N, Nakagawa H, Ishii A (2003) A mouse model of the atopic eczema/dermatitis syndrome by repeated application of a crude extract of housedust mite Dermatophagoides farinae. Allergy. 58(2): 139–145.

Schleich FN, Chevremont A, Paulus V, Henket M, Manise M, Seidel L, Louis R (2014) Importance of concomitant local and systemic eosinophilia in uncontrolled asthma. Eur Respir J. 44(1): 97-108.

Virchow JC Jr, Kroegel C, Walker C, Matthys H (1994) Cellular and immunological markers of allergic and intrinsic bronchial asthma. Lung. 172(6): 313–334.

Pelaia G, Vatrella A, Busceti MT, Gallelli L, Calabrese C, Terracciano R, Maselli R (2015) Cellular mechanisms underlying eosinophilic and neutrophilic airway inflammation in asthma. Mediators Inflamm. 2015: 879783.

Zeiger RS, Schatz M, Li Q, Chen W, Khatry DB, Gossage D, Tran TN (2014) High blood eosinophil count is a risk factor for future asthma exacerbations in adult persistent asthma. J Allergy Clin Immunol Pract. 2(6): 741–750.

Schleich FN, Louis R (2014) Importance of concomitant local and systemic eosinophilia in uncontrolled asthma. Eur Respir J. 44(4): 1098–1099.

Bohinc T, Vayias B, Bartol T, Trdan S (2013) Assessment of Insecticidal Efficacy of Diatomaceous Earth and Powders of Common Lavender and Field Horsetail against Bean Weevil Adults. Neotrop Entomol. 42(6): 642–648.

Luz C, Rodrigues J, Rocha LF (2012) Diatomaceous earth and oil enhance effectiveness of Metarhizium anisopliae against Triatoma infestans. Acta Trop. 122(1): 29–35.

Andric GG, Markovic MM, Adamovic M,

Dakovic A, Golic MP, Kljajic PJ (2012) Insecticidal potential of natural zeolite and diatomaceous earth formulations against rice weevil (Coleoptera: Cur-culionidae) and red flour beetle (Coleoptera: Tenebrionidae). J Econ Entomol. 105(2): 670–678.

Bennett DC, Yee A, Rhee YJ, Cheng KM (2011) Effect of diatomaceous earth on parasite load, egg production, and egg quality of free-range organic laying hens. Poult Sci. 90(7): 1416–1426.

Park R, Rice F, Stayner L, Smith R, Gilbert S, Checkoway H (2002) Exposure to crystalline silica, silicosis, and lung disease other than cancer in diatomaceous earth industry workers: a quantitative risk assessment. Occup Environ Med. 59(1): 36–43.

Published
2019-06-02
How to Cite
1.
Liu S-Y, Chang Y-H, Ji H-R, Chiu C-D. Effects of Diatomaceous Earth on House Dust Mite Sensitization in BALB/c Mice. J Arthropod Borne Dis. 13(2):177-184.
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Original Article