Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 12 4 2018 10 12 361 369 Mahmoud Osanloo Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Students’ Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran Mohammad Mehdi Sedaghat Department of Medical Entomology and Vector Control, School of Public Health, Tehran Universi-ty of Medical Sciences, Tehran, Iran Fariba Esmaeili Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran Amir Amani Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran AND Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran 2018 12 05 Background: In this study, larvicidal activity of clove essential oil (EO), as a green and relatively potent larvicide, was compared with its main constituent, Eugenol, against Anopheles stephensi. Methods: High-performance liquid chromatography (HPLC) was used to determine the amount of eugenol, major constituent of clove EO. In addition, larvicidal activity of clove EO and eugenol was evaluated against An. stephensi. Results: The amount of eugenol in clove EO was determined as 67% using HPLC analysis. LC50 and LC90 of clove EO (57.49 and 93.14ppm, respectively) were significantly lower than those of eugenol (86.96 and 128.18 ppm, re­spectively). Conclusion: EO showed more effective than its major component. Considering the lower cost of the essential oil and lower risk in occurrence of resistance in larvae, use of clove EO is preferred as larvicide in comparison with eugenol, against An. stephensi. https://jad.tums.ac.ir/index.php/jad/article/view/1019 https://jad.tums.ac.ir/index.php/jad/article/download/1019/423