Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 14 2 2020 05 03 1311 1311 Masoumeh Amin Department of Medical Entomology and Vector Control, School of Health, Tehran University of Medical Sciences, Tehran, Iran AND Department of Medical Entomology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran Morteza Zaim Department of Medical Entomology and Vector Control, School of Health, Tehran University of Medical Sciences, Tehran, Iran Hamideh Edalat Department of Medical Entomology and Vector Control, School of Health, Tehran University of Medical Sciences, Tehran, Iran Hamid Reza Basseri Department of Medical Entomology and Vector Control, School of Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Yaghoobi-Ershadi Department of Medical Entomology and Vector Control, School of Health, Tehran University of Medical Sciences, Tehran, Iran Farhad Rezaei Department of Medical Virology, School of Health, Tehran University of Medical Sciences, Tehran, Iran Kourosh Azizi Department of Medical Entomology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran Mostafa Salehi-Vaziri Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran, Tehran, Iran Mohsen Ghane Department of Clinical Sciences, School of Veterinary Science, Shiraz University, Shiraz, Iran Saideh Yousefi-Sharikabad Department of Medical Entomology and Vector Control, School of Health, Tehran University of Medical Sciences, Tehran, Iran Sorna Dabaghmanesh Department of Medical Entomology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran Sedigheh Kheirandish Department of Oral and Maxillofacial Pathology, Bushehr University of Medical Sciences, Bushehr, Iran Mohammad Esmaeil Najafi Environmental Health Unit, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, Iran Jalal Mohammadi Department of Medical Entomology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran 2020 06 27 Background: West Nile Virus, a mosquito-borne flavivirus, causes a variety of symptoms in human, from asymp­tomatic infection to neuroinvasive disease. Several studies have been conducted on the seroprevalence of WNV in­fection in dif­ferent areas from Iran. This study was performed to find the presence of antiviral antibodies in human serum among some high risk population and awareness of health care staff about symptom of the WNV infection. Methods: Study performed in five geographical districts based on high population of immigrant and do­mestic birds and prevalence of the antiviral antibodies in horses which was reported previously. Totally 150 human blood sam­ples were collected during 2018. The samples collected from patients referred to the clinics. The ELISA method used to detect IgG and IgM antibody against WNV. Logistic regression models used to analyze the effect of sex, age, keeping birds and urban/rural residence on the risk of infection. The awareness of health care staff about symp­tom of infection surveyed. Results: From all blood donors, 41 samples (27.33%) showed positive to IgG antibody. From which 56.10% were males and remaining females. None of the mentioned factors had a significant relationship. Health care staff had less attention to the infection.  Conclusions: Although the prevalence of antibodies was relatively high, due to the similarity to other viral diseases, health care staff had less attention to the disease. The study showed that people in these areas have been exposed to the virus. Further research activities are recommended for control of this arbovirus. https://jad.tums.ac.ir/index.php/jad/article/view/1311 https://jad.tums.ac.ir/index.php/jad/article/download/1311/497