Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 9 2 2015 10 11 204 214 EN Sumrin Sahar Lab of Parasitology, Zoology Department, University of the Punjab, Lahore, Pakistan. Akhtar Tanveer Lab of Parasitology, Zoology Department, University of the Punjab, Lahore, Pakistan. Akbar Ali Department of Basic Health Sciences, Faculty of Pharmacy, Northern Border University, Rafha, Saudi Arabia. Hazrat Bilal Medical Entomology and Disease Vector Control, Health Services Academy, Lahore, Pakistan. Rana Muhammad Saleem Medical Entomology and Disease Vector Control, Health Services Academy, Lahore, Pakistan. 2015 10 10 2015 10 10 Background: The aim of the study was to identify the prevalence of different species of Plasmodium and haplotypes of pfcrt in Plasmodium falciparum from the selected area. Methods: Overall, 10,372 blood films of suspected malarial patients were examined microscopically from rural health center Sinawan, district Muzaffargarh, Pakistan from November 2008 to November 2010. P. falciparum positive samples (both whole blood and FTA blood spotted cards) were used for DNA extraction. Nested PCR was used to amplify the pfcrt (codon 72–76) gene fragment. Sequencing was carried out to find the haplotypes in the amplified fragment of pfcrt gene. Result: Over all slide positivity rate (SPR), P. vivax and P. falciparum positivity rate was 21.40 %, 19.37 % and 2.03% respectively. FTA blood spotted cards were equally efficient in the blood storage for PCR and sequencing. Analysis of sequencing results of pfcrt showed only one type of haplotype SagtVMNT (AGTGTAATGAATACA) from codon 72–76 in all samples. Conclusion: The results show high prevalence of CQ resistance and AQ resistant genes. AQ is not recommended to be used as a partner drug in ACT in this locality, so as to ward off future catastrophes. https://jad.tums.ac.ir/index.php/jad/article/view/132 https://jad.tums.ac.ir/index.php/jad/article/download/132/120