Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 14 3 2020 07 30 1366 1366 Yavar Rassi Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Eslam Moradi-Asl Department of Public Health, School of Public Health, Ardabil University of Medical Sciences, Ardabil, Iran AND Arthropod Borne Diseases Research Center, Ardabil University of Medical Sciences, Ardabil, Iran Hassan Vatandoost 1Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Department of Chemical Pollutants and pesticides, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran Malek Abazari Department of Public Health, School of Public Health, Ardabil University of Medical Sciences, Ardabil, Iran Abedin Saghafipour Department of Public Health, School of Public Health, Qom University of Medical Sciences, Qom, Iran 2020 10 13 Background: Phlebotomus kandelakii and Phlebotomus perfiliewi transcaucasicus sand flies are the vectors of vis­ceral leishmaniasis in Iran. The aim of this study was to evaluate the susceptibility of Ph. kandelakii and Ph. perfil­iewi trans­caucasicus, collected from an endemic focus of visceral leishmaniasis (VL) in the northwest of Iran, to different select­ed insecticides. Methods: Sand flies were collected from the villages of Meshkinshahr and Germi Counties using light traps and aspira­tors from May to October 2019. The sand flies were identified as Ph. kandelakii and Ph. perfiliewi transcauca­sicus us­ing standard identification keys. Susceptibility test was carried out against DDT (4%), Malathion (5%), Propoxur (0.1%) and Lambda-cyhalothrin (0.05%) according to the WHO standard method. MedCalc statistical software was employed to calculate LT50 and LT90 and to compare the chances of sand flies surviving the exposure to the studied in­secticides. Results: A total of 1,278 female specimens were used for the susceptibility tests. Out of which 1,063 samples were used for the test and 215 for the control group. The estimated LT50 values for DDT (4%), Malathion (5%), Propoxur (0.1%), and Lambda-cyhalothrin (0.05%) for Ph. kandelakii were 15.1, 13.4, 15.4 and 5.8 minutes respectively, and for Ph. per­filiewi transcaucasicus were 11.9, 15.6, 15.9 and 5.8 minutes  respectively. Conclusion: This susceptibility studies revealed different LT50 values for different insecticides efficient against Ph. kandelakii and Ph. perfiliewi transcaucasicus. The regular monitoring for the resistance of Ph. kandelakii and Ph. per­filiewi transcaucasicus sand flies seems necessary in diseases control programs in this area. https://jad.tums.ac.ir/index.php/jad/article/view/1366 https://jad.tums.ac.ir/index.php/jad/article/download/1366/511