Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 6 2 2015 10 14 104 111 EN Hazrat Bilal Medical Entomology and Disease Vector Control, Health Services Academy, Islamabad-Pakistan Waseem Akram Akram Department of Agricultural Entomology, University of Agriculture Faisalabad-Pakistan. Soaib Ali-Hassan Medical Entomology and Disease Vector Control, Health Services Academy, Islamabad-Pakistan 2015 10 14 2015 10 14 Background: Development of insecticide resistance occurred due to the continuous and misuse of synthetic insecti- cides therefore, the recent study was conducted to explore eco-friendly plant extracts that have some potential to sup- press mosquito larval population. Methods: WHO recommended mosquito larval bioassay method for insecticide was used while for the analysis of cit- rus oils for limonin and nomilin content HPLC was used. Results: Among the two citrus cultivars tested as larvicide against Aedes albopictus, valencia late (Citrus sinensis) was the best in terms of LC50 (297 ppm), % mortality (97%) and LT50 (18.49 hours) then freutrall early (Citrus reticulate) with LC50 (377.4 ppm), % mortality (88%) and LT50 (31 hours), While nomilin gave lowest LC50 (121.04 ppm) than limonin (382.22 ppm) after 72 hours of exposure. Valencia late also had more limonin and nomilin (377 µ g/ml and 21.19 µ g/ml) than freutrall early (5.29 µ g/ml and 3.89 µ g/ml) respectively. Conclusion: Valencia late showed best results in term of LC50, LT50 and percentage mortality against Aedes albopictus as it has more amount of nomilin then freutrall early, however further evaluation in the field conditions is required. https://jad.tums.ac.ir/index.php/jad/article/view/163 https://jad.tums.ac.ir/index.php/jad/article/download/163/147