Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 18 2 2024 11 16 149 156 Pedram Talaie Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Maryam Tavassoli Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ehsan Mostafavi Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran, National Reference Laboratory for Plague, Tularemia and Q Fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar-Ahang, Hamadan, Iran Zakiyeh Telmadarraiy Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mahdi Rohani Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran Saber Esmaeili Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran, National Reference Laboratory for Plague, Tularemia and Q Fever, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Akanlu, Kabudar-Ahang, Hamadan, Iran Mohammad Mehdi Sedaghat Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2023 05 09 2023 07 29 Background: Coxiella burnetii causes Q fever, a zoonotic and vector-borne disease. Ticks serve as vectors for this bac­terium. This study aimed to determine the prevalence of C. burnetii infection in ticks in Shahr-e-Rey County, Tehran Province. Methods: From December 2016 to November 2017, 179 ticks were collected on sheep at animal husbandry facilities and slaughterhouses located in Shahr-e-Rey, Tehran Province. Tick samples were morphologically identified and evalu­ated for the presence of the C. burnetii IS1111 gene using real-time PCR. Results: Ticks were classified into four genera: Hyalomma (66.48%), Rhipicepalus (23.47%), Dermacentor (7.26%), and Ornithodoros (2.79%). Furthermore, 35.20% of the ticks were Hyalomma nymphs. All 77 ticks were pooled by species, and C. burnetii was found in 22.08% (n= 17). Ornithodoros lahorensis was the most prevalent tick infected with C. burnetii. Conclusion: The distribution of C. burnetii and reports of Q fever from various regions of the country strongly suggest that the monitoring system should give this disease more attention. https://jad.tums.ac.ir/index.php/jad/article/view/1647 https://jad.tums.ac.ir/index.php/jad/article/download/1647/661