Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 19 3 2025 12 29 1696 1696 Jamal Sarvari Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Bahador Sarkari Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Fatemeh Osooreh Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Mehdi Fazlalipour Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran Mohammad Hassan Pouriayevali Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran Tahmineh Jalali Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran Mohammad Javad Ranjbar Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Mostafa Salehi-Vaziri Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran, Research Center for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran 2023 10 24 2025 12 21 Background: Hantaviruses are mainly transmitted to humans through the inhalation of aerosolized excreta from infect­ed rodent reservoirs. The present study was conducted to analyze the prevalence of hantavirus infection among rodents in the Boyer-Ahmad region. Methods: A total of 52 rodents were captured in the Boyer-Ahmad region of Kohgiluyeh and Boyer-Ahmad Province during June to November 2014, using Sherman live traps. Blood and tissue samples were obtained from the heart and lungs, respectively.  Hantavirus Pool 1 "Eurasia" IgG and Pool 2 "America" ELISA IgG kits were used to detect IgG antibodies against both Old World and New World hantaviruses. Moreover, total RNA extraction was performed on the lung tissue, and a pan-hantavirus nested RT-PCR was conducted to detect hantavirus RNA. Results: Meriones persicus was the most abundant species (n=25, 48%). The results of the ELISA showed that all the serum samples from the rodents were negative for antibodies against both Eurasian and American hantaviruses. Moreo­ver, no rodent tissue samples tested positive for the hantavirus RNA by the pan-hantavirus RT-PCR. Conclusion: Although no hantavirus infection was detected in this study, the presence of hantavirus reservoirs in Koh­giluyeh and Boyer-Ahmad Province suggests that hantavirus circulation cannot be completely ruled out. Further studies with a larger sample size are recommended. https://jad.tums.ac.ir/index.php/jad/article/view/1696 https://jad.tums.ac.ir/index.php/jad/article/download/1696/703