Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 19 1 2025 09 14 1712 1712 Asghar Yadollahi Department of Biology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran Aboozar Soltani Department of Biology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran Ahmad Gholami Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Azim Paksa Department of Biology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran Kourosh Azizi Department of Biology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran 2024 02 06 2025 08 18 Introduction: Mosquitoes transmit several diseases to humans. New and environmentally safe methods are needed to control mosquito populations effectively. This study aimed to evaluate the larvicidal and repellent properties of extracts and silver nanoparticles synthesized from Zataria multiflora (Shirazi thyme). Methods: To determine the larvicidal and repellent properties of Z. multiflora, we tested different concentrations of synthesized silver nanoparticles and crude extract against this malaria vector. Results: The crude extract exhibited weak larvicidal activity, with an LC50 of 616.52 μg/mL and an LC90 of 1109.74 μg/mL. However, the synthesized silver nanoparticles are highly active on the larvae of Anopheles stephensi, with an LC50 at 51.07 μg/mL and an LC90 at 119.04 μg/mL. Both the crude extract (ED50: 0.00031 μg/cm²; ED90: 0.0018 μg/ cm²) and silver nanoparticles (ED50: 0.000072 μg/cm²; ED90: 0.00048 μg/cm²) demonstrated significant repellent activ­ity. Particle size analysis revealed a range of 24.56 to 63.76 nanometers, with an average size of 52.7 nanometers. Conclusion: This study showed that Z. multiflora silver nanoparticles have significant larvicidal properties, so they are suggested as a nature-friendly larvicide. https://jad.tums.ac.ir/index.php/jad/article/view/1712 https://jad.tums.ac.ir/index.php/jad/article/download/1712/681