Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 19 1 2025 09 24 1754 1754 Pradyumna Chakraborty Department of Veterinary Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, India Sulanki Sarkar Department of Veterinary Parasitology, Seleish, CAU, Aizawl, India Sudip Das Department of Veterinary Parasitology, Ranchi Veterinary College, Kanke, Ranchi, India Bishal Debbarma Department of Veterinary Medicine, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, India Shubhamitra Chaudhuri Department of Veterinary Clinical Complex, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, India 2024 08 13 2025 08 27 Background: Trypanosomosis is one of the most important and deadliest diseases of canines. Usually within 3 to 4 weeks an infected dog dies in the acute form of the disease. The use of inappropriate doses of anti-trypanosomatid drugs in animals leads to the development of huge resistance in the organisms. Methods: The present study communicates the successive therapeutic management of clinical trypanosomiasis with five doses of diminazine aceturate injection. One mongrel dog came with the clinical signs of persistent fever, tachycar­dia, severe dehydration, pale mucous membrane, corneal opacity, prolonged recumbency, and enlargement of superfi­cial lymph nodes. A Giemsa-stained thin blood smear revealed the presence of Trypanosoma organisms. Haematobio­chemical parameters revealed lowered total leucocyte count, haemoglobin, total platelet count, serum total protein, and blood glucose levels and elevated levels of alkaline phosphatase, serum creatinine, and blood urea nitrogen. Results: We observed the animal responded after the third day of post-therapy, and the clinical and haematobiochemi­cal improvement was recorded after twenty days of post-therapy. The corneal opacity completely subsides after 28 days consequently. Conclusions: During our study we found that the proper significant dosing of diminazine aceturate can cure the Trypa­nosomosis in dogs. https://jad.tums.ac.ir/index.php/jad/article/view/1754 https://jad.tums.ac.ir/index.php/jad/article/download/1754/683