Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 19 2 2025 10 28 113 124 EN Mohammad Nasrabadi Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Abolghasemi Dehaqani School of Electrical and Computer Engineering, College of Engineering, University of Tehran, Tehran, Iran, School of Cognitive Sciences, Institute for Research in Fundamental Sciences (IPM), Tehran, Iran Hassan Vatandoost Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Mehdi Sedaghat Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Amrollah Azarm Department of Medical Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran seyed hassan Moosakazemi Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2024 11 19 2025 05 20 Background: Diseases such as malaria are transmitted by Anopheles species, among which Anopheles stephensi is one of the most important malaria vectors in Iran. Reducing the transmission of mosquito-borne diseases depends on con­trolling the mosquito vector or minimizing human-vector contact. A promising method for control, surveillance, and monitoring involves using synthetic attractants in traps to target vectors. This study aims to determine the effective dose of octenol, isovaleric acid, lactic acid, hexanoic acid, isoamyl alcohol, myristic acid, and ammonium hydrogen bicar­bonate using the high-throughput screening system (HTSS) device in the laboratory. Methods: After rearing An. stephensi in the insectary, High-Throughput Screening System (HTSS) assay was used to obtain the 50% and 90% effective dose (ED) of the attractive compounds. Probit analysis was used to analyze the re­sults and prepare the regression lines of ED50 and ED90. Results: This study showed that An. stephensi had the highest absorption to isoamyl alcohol (ED50= 0.57 mL/L, ED90= 1.04 mL/L), followed by isovaleric acid (ED50= 1.96 mL/L, ED90= 3.00 mL/L), myristic acid (ED50= 24.77 g/L, ED90= 47.08 g/L), octenol (ED50= 26.64 mL/L, ED90= 54.36 mL/L) and lactic acid (ED50= 54.98 mL/L, ED90= 132.9 mL/L), while hexanoic acid (ED50= 87.50 mL/L, ED90= 244.49 mL/L) per liter and ammonium hydrogen bicarbonate (ED50= 93.84 g/L, ED90= 234.01 g/L) showed the lowest absorption rate. Conclusion: Our laboratory results identified isoamyl alcohol and isovaleric acid as highly effective attractants for An. stephensi. These compounds are strong candidates for inclusion in field-deployable traps after further validation. https://jad.tums.ac.ir/index.php/jad/article/view/1783 https://jad.tums.ac.ir/index.php/jad/article/download/1783/689