Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 19 3 2026 01 28 1866 1866 EN Saeedeh Ebrahimi Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Hassan Vatandoost Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Ali Oshaghi Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Kourosh Azizi Department of Medical Entomology and Vector Control, Research Center for Health Sciences, Institute of Health, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran Mahnaz Khanavi Department of Pharmacognosy, School of Pharmacy and Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran Seyed Hassan Moosa-Kazemi Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2025 08 29 2026 01 27 Background: The presence of Aedes aegypti in Iran and the risk of transmitting viral diseases, such as dengue and Zika, underscore the importance of evaluating effective vector control methods. Methods: This study investigated the effectiveness of diflubenzuron, an insect growth regulator that inhibits chitin syn­thesis, in preventing adult emergence of Ae. aegypti larvae from Hormozgan. The concentrations of 0.03, 0.06, 0.12 and 0.24 mg/L of 25% wettable powder formulations of diflubenzuron were used, according to WHO protocols. Results: Diflubenzuron was highly effective at 0.24 mg/L, achieving 98% emergence inhibition (EI50=0.053 mg/L, EI90=0.146 mg/L). At lower concentrations, e.g., 0.12 mg/L, emergence inhibition decreased to 83%, indicating a clear concentration-dependent reduction in efficacy. Larvae exposed to sublethal doses displayed abnormal movements and reduced responses to stimuli. Conclusion: This experiment supports diflubenzuron’s potential as an effective larvicide for vector control programs in Iran. However, field trials and resistance monitoring are recommended before widespread implementation. https://jad.tums.ac.ir/index.php/jad/article/view/1866 https://jad.tums.ac.ir/index.php/jad/article/download/1866/706