Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 0 0 2026 07 22 1966 1966 Tahereh Asgarian Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Seyed Hassan Moosa-Kazemi Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ahmadali Enayati Department of Medical Entomology and Vector Control, School of Public Health, Mazandaran University of Medical Sciences, Sari, Iran, Health Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran Mohammad Ali Oshaghi Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Mehdi Sedaghat Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2026 06 16 2026 07 19 Background: Aedes aegypti is a major vector of numerous arboviruses, including dengue, Zika, chikungunya and yel­low fever. This study aimed to establish a standardized and practical procedure for maintaining and propagating an Ae. aegypti colony under controlled insectary conditions in Bandar Abbas, Iran. Methods: Environmental parameters were routinely monitored. Larvae were reared with an appropriate diet under a regular feeding schedule, while adult mosquitoes were provided with a sucrose solution and blood meals. The correla­tion between the duration of egg paper drying and the hatching percentage was evaluated. Hatching rates were com­pared across three media: cooled boiled water, yeast and larval food. Results: A strong negative correlation was observed between drying duration and hatching percentage, with a Pearson correlation of −0.818 (p< 0.001). Gradual drying of egg papers over a period of two days resulted in a hatching rate of over 90% without any hatching stimulant. No significant differences in hatching rates were found among the three me­dia, as indicated by F= 0.601 (p= 0.578). In a novel observation, female mosquitoes were found to deposit ap­proxi­mately 20−30% of their eggs directly on the water surface inside ovitraps under insectary conditions in Iran. Conclusion: This study provides a reliable, low‑cost model for rearing Ae. aegypti in research insectaries and facilitates future studies on vector control. https://jad.tums.ac.ir/index.php/jad/article/view/1966 https://jad.tums.ac.ir/index.php/jad/article/download/1966/729