Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 7 1 2013 06 15 94 98 EN Mehdi Badakhshan Department of Medical Parasitology and Entomology, College of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Javid Sadraei Department of Medical Parasitology and Entomology, College of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Vahideh Moin-Vaziri Department of Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2015 10 16 Background: Stigmaeids mites have been recorded only on Phlebotominae sand flies up to now. Five species of Eustigmaeus, and three of Stigmaeus were reported on infested sandflies in different country up to the present. Methods: Sand flies collection was done using CDC light trap and sticky paper. The mites were isolated from in- fested specimens, mounted in Puri’s medium and identified using reliable keys. Results: A mite infested Phlebotomus papatasi was observed during a study on sandflies of one of the southern provinces of Iran, near to the Persian Gulf. Several scars resulting from mite attachment were found on abdominal tergites of this female sand fly. The mites were identified as Eustigmaeus johnstoni. Conclusion: This parasitic mite is one of the eyeless species, which has a great distribution over the world, reported from Tunisia, Saudi Arabia, Pakistan, Yemen, Cyprus and Palestine. But, this is the first record of this species from Iran. https://jad.tums.ac.ir/index.php/jad/article/view/246 https://jad.tums.ac.ir/index.php/jad/article/download/246/222