Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 10 4 2016 10 24 546 559 EN Hafiz Muhammad Ashraf Department of Zoology, Government College University Faisalabad, Pakistan Muhammad Kashif Zahoor Department of Zoology, Government College University Faisalabad, Pakistan Shabab Nasir Department of Zoology, Government College University Faisalabad, Pakistan Humara Naz Majeed Department Pharmaceutical and Biological Chemistry, School of Pharmacy, University College London, UK Sarwat Zahoor Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad, Pakistan 2015 12 20 2015 12 20 Background: Keeping in view the havoc situation of dengue fever in Pakistan, the current study was designed to demon­strate the genetic variations, gene flow and rate of migration from Lahore and Faisalabad. Methods: The larvae were collected from both natural and artificial breeding places from each collection site. The adult mosquitoes were collected by means of sweep net and battery-operated aspirator. DNA extraction was per­formed using TNE buffer method. Ten GeneLink-A series RAPD primers were used for PCR amplification and the data was analyzed through POPGENE. Results: The number of amplification products produced per primer varied from 8-12, ranging from 200 to 2000 bp with an average of 10.0 bands per primer. The percentage of polymorphic loci amplified by each primer varied from 22.5 to 51%. The UPGMA dendrogram demonstrates two distinct groups from Faisalabad and Lahore populations. The genetic diversity ranged from 0.260 in Faisalabad to 0.294 in Lahore with a total heterozygosity of 0.379. The GST value for nine populations within Lahore was 0.131 (Nm= 3.317), whereas for nine populations in Faisalabad GST value was 0.117 (Nm= 3.773). The overall genetic variation among eighteen populations showed GST= 0.341 and Nm= 1.966. Conclusion: The genetic relatedness and Nm value show that Ae. aegypti populations exhibit intra-population gene flow both in Faisalabad and Lahore. Although, both cities show a distinct pattern of genetic structure; however, few areas from both the cities show genetic similarity. The gene flow and the genetic relatedness in few populations of Lahore and Faisalabad cities need further investigation. https://jad.tums.ac.ir/index.php/jad/article/view/344 https://jad.tums.ac.ir/index.php/jad/article/download/344/297