Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 10 4 2016 10 24 577 585 EN Alireza Sanei-Dehkordi Department of Medical Entomology and Vector Control, Faculty of Health, Hormozgan Univer-sity of Medical Sciences, Bandar Abbas, Iran AND Social Determinants in Health Promotion Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran Mohammad Mehdi Sedaghat Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Hassan Vatandoost Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Abai Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2016 01 27 2016 01 27 Background: Recently, essential oils and extracts derived from plants have received much interest as potential bioactive agents against mosquito vectors. Methods: The essential oils extract from fresh peel of ripe fruit of Citrus aurantium and Citrus paradisi were tested against mosquito vector Anopheles stephensi (Diptera: Culicidae) under laboratory condition. Then chemical com­position of the essential oil of C. aurantium was analyzed using gas chromatography-mass spectrometry (GC–MS). Results: The essential oils obtained from C. aurantium, and C. paradisi showed good larviciding effect against An. stephensi with LC50 values 31.20 ppm and 35.71 ppm respectively. Clear dose response relationships were established with the highest dose of 80 ppm plant extract evoking almost 100% mortality. Twenty-one (98.62%) constituents in the leaf oil were identified. The main constituent of the leaf oil was Dl-limonene (94.81). Conclusion: The results obtained from this study suggest that the limonene of peel essential oil of C. aurantium is promising as larvicide against An. stephensi larvae and could be useful in the search for new natural larvicidal compounds. https://jad.tums.ac.ir/index.php/jad/article/view/367 https://jad.tums.ac.ir/index.php/jad/article/download/367/302