Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 11 1 2017 04 18 10 18 EN Mohammad Saiful Islam Laboratory of Veterinary Parasitology, College of Veterinary Medicine and Bio-safety Research Centre, Chonbuk National University, Jeonju, Republic of Korea AND Department of Medicine Surgery and Obstetrics, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh Myung Jo You Laboratory of Veterinary Parasitology, College of Veterinary Medicine and Bio-safety Research Centre, Chonbuk National University, Jeonju, Republic of Korea 2016 02 14 2016 02 14 Background: The aim of the present study was to induce salivation in Haemaphysalis longicornis to increase saliva production and to characterize the collection of proteins present in the collected saliva using on-chip-electrophoresis. Methods: Saliva of adult female engorged H. longicornis was collected by treatment with 0.2% dopamine hydrochlo­ride. All protein samples were characterized by SDS-PAGE electrophoresis using a microfluidic High Sensitiv­ity Protein Assay 250 kit by 2100 Bioanalyzer (Agilent Technologies, USA) under non-reducing conditions. Results: The average salivary protein concentration was 0.169 µg/µl/tick and saliva secretion decreased with in­creased time of tick detachment from the host. Saliva secretion volume increased to 3.56 µl in the group of ticks with a body weight between 301–350 mg as compared to higher and lower body weight groups. On-chip-electrophoresis results show 13 distinct bands ranging from 9.9 to 294 kDa. Conclusion: Based on molecular weight, the putative salivary proteins are comprised of proline-rich proteins, tria­bin, apyrase members of the 12-kDa protein family, platelet inhibitors and anti-inflammatory proteins as tick saliva contains anti-inflammatory components. https://jad.tums.ac.ir/index.php/jad/article/view/384 https://jad.tums.ac.ir/index.php/jad/article/download/384/305