Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 11 1 2017 04 18 Chemical Constitute and Larvicidal Activity of Fractions of Ajuga chamaecistus tomentella Plant against Malaria Vector Anopheles stephensi 116 123 EN Mahnaz Khanavi Department of Pharmacognosy and Persian Medicine and Pharmacy Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Behnaz Najafi Department of Pharmacognosy and Persian Medicine and Pharmacy Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Seyede Nargess Sadati Department of Traditional Pharmacy, School of Traditional Iranian Medicine, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Abai Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Hassan Vatandoost Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2016 06 19 2016 06 19 Backgrand: The genus Ajuga, belongs to Lamiaceae family, is one of the exclusive subspecies in the flora of Iran. The plants of this genus are used traditionally for treatment of joints pain, gout, jaundice, and as insecticide. Methods: larvicidal activity of methanol 80% extract and partition fractions of hexane, chloroform, and ethyl acetate obtained from aerial parts of Ajuga chamaecistus subspecies tomentella against malaria vector An. stephensi was evaluated. Phytochemical study of active fraction was analyzed using column chromatography and spectroscopy. Results: According to the results, among different fractions, hexane fraction has the most larvicidal activity with mortality rate of 100% in concentration of 102 ppm and LC50 of 95.66ppm. The structure of compound 1, main phy­toecdysteroid compound separated from hexane fraction, was determined to be ajugalide-E. Conclusion: The results suggested that the hexane fraction of Ajuga chamaecistus subsp tomentella could be used as a natural and biodegradable insecticide. https://jad.tums.ac.ir/index.php/jad/article/view/483 https://jad.tums.ac.ir/index.php/jad/article/download/483/320