Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 11 2 2017 06 13 226 235 EN Shahid Iqbal Mohammed School of Life Sciences, North Maharashtra University, Jalgaon, India Kishor Sukhlal Vishwakarma School of Life Sciences, North Maharashtra University, Jalgaon, India Vijay Laxminarayan Maheshwari School of Life Sciences, North Maharashtra University, Jalgaon, India 2016 08 06 2016 08 06 Background: To study the chemical constituents and larvicidal activity of essential oil extracted from the leaves of Coccinia grandis against three mosquito species. Methods: Essential oil was extracted by hydro distillation using clevenger apparatus and was analyzed for chemical constituents by gas chromatography-mass spectrophotometry (GC-MS). Larvicidal activity was recorded after 12 and 24h of post-exposure against three mosquito species, Anopheles stephensi, Aedes aegypti and Culex quinquefascia­tus. Dead larvae were identified when they failed to move after probing with a needle in the siphon or cervical re­gion. The LC50 and LC90 values for three mosquito larvae were calculated by Probit analysis. Results: The GC-MS analysis revealed that essential oil contains 23 different constituents. Out of these 23 constitu­ents, major constituents identified were n-tetracosane (39.18%), n-eicosane (30.04%), tetratriacotane (2.97%), 7-oc­tadecanal (2.81%), and tricosane (2.31%). Essential oil from leaves of Coccinia grandis exhibited significant larvi­cidal activity against An. stephensi with LC50 and LC90 values 39.41ppm and 123.24ppm, respectively. This was fol­lowed by Ae. aegypti and Cx. quinquefasciatus with LC50 and LC90 values of 48.20ppm, 131.84ppm and 52.80ppm, 135.48ppm, respectively after 24h of exposure. Conclusion: The results could be useful in developing a cost effective, ecofriendly, region specific and practical strategy for the control of mosquito vectors. https://jad.tums.ac.ir/index.php/jad/article/view/519 https://jad.tums.ac.ir/index.php/jad/article/download/519/330