Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 4 2 2010 12 15 19 25 EN A Barmaki Dept. of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medi- cal Sciences (TUMS), Tehran, Iran. J Rafinejad Dept. of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medi- cal Sciences (TUMS), Tehran, Iran. H Vatandoost Dept. of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medi- cal Sciences (TUMS), Tehran, Iran. Z Telmadarraiy Dept. of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medi- cal Sciences (TUMS), Tehran, Iran. F Mohtarami Dept. of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medi- cal Sciences (TUMS), Tehran, Iran. SH Leghaei Health Management Office of Qazvin Province, Qazvin University of Medical Sciences, Qazvin, Iran MA Oshaghi Dept. of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medi- cal Sciences (TUMS), Tehran, Iran. 2015 10 03 Background: A molecular survey was conducted to investigatethe presence of pathogenic Borrelia persica species caus­ing the tick borne relapsing fever (TBRF) in Takistan district Qazvin Province, western Iran. Methods: A number of 1021 soft ticks were collected from 31 villages including previously reported infected and none-infected TBRF cases and individually examined for the presenceof B. persica DNA by conventional PCR target­ing the 16SrRNA. Results: A total of 1021 soft ticks of three species of Ornithodouros tholozani (120: 11.75%), O. lahorensis (461: 45.15%) and Argas persicus (440: 43.1%) were collected and tested against Borrelia infection. Soft ticks were more preva­lent (67%) in infected areas than none infected areas. The rate O. tholozani in infected areas was much greater (29 times) than none infected areas. Ninety seven percent of soft ticks in none infected areas were of O. tholozani. Six­teen (16.7%) ticks of tested (n=95) O. tholozani were infected with B. persica. Three (1.3%) out of 205 soft ticks of O. lahorensis were positive for Borrelia sp., and no infection was observed in A. persicus. TaqI RFLP analysisand se­quence analysis of the positive PCR products showed the presence of B. persica. The RFLP analysis showed that the positive ticks of O. lahorensis were infected with unknown Borrelia species. Conclusion: This study showed that although there were no TBRF cases in Takisan, but still infected O. tholozani, the known vector of TBRF, presented in the region. Control measures needs to be fulfilled in Thakisan.  https://jad.tums.ac.ir/index.php/jad/article/view/69 https://jad.tums.ac.ir/index.php/jad/article/download/69/67