Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 12 1 2018 03 18 85 93 EN Hassan Vatandoost Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Department of Chemical Pollutants and Pesticides, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran Arezoo Rustaie Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research Centre, Tehran University of Medical Sciences, Tehran, Iran Zeynab Talaeian Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research Centre, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Abai Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Fatemeh Moradkhani Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research Centre, Tehran University of Medical Sciences, Tehran, Iran Mahdi Vazirian Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research Centre, Tehran University of Medical Sciences, Tehran, Iran Abbas Hadjiakhoondi Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research Centre, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Shams-Ardekani Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research Centre, Tehran University of Medical Sciences, Tehran, Iran AND Department of Traditional Pharmacy, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran Mahnaz Khanavi Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research Centre, Tehran University of Medical Sciences, Tehran, Iran AND Faculty of Land and Food Systems, University of British Columbia, Vancouver, Canada 2018 03 18 2018 03 18 Background: Malaria, a mosquito-transmitted disease, is still a major human health problem all over the world. Lar­viciding is a component of comprehensive control program to overcome the disease. Negative aspects of synthetic insecticides application, such as environmental safety concerns, have favored use of natural insecticides. Methods: Larvicidal activity of essential oil, extracts and fractions of a wild grown and a cultivated type of Bunium persicum fruits against malaria vector Anopheles stephensi was assessed according to the method described by WHO. Results: Bunium persicum showed remarkable potency against An. stephensi larvae. LC50 values for essential oil, total extract, petroleum ether fraction and methanol fraction were 27.4284, 64.9933, 85.9933 and 255.7486ppm for wild type, and 21.3823, 63.2580, 62.7814 and 152.6357ppm for cultivated one. Conclusion: The results of this study suggest B. persicum as a valuable source of natural insecticides against malaria vector Anopheles stephensi. https://jad.tums.ac.ir/index.php/jad/article/view/837 https://jad.tums.ac.ir/index.php/jad/article/download/837/376