Tehran University of Medical Sciences Journal of Arthropod-Borne Diseases 2322-1984 12 2 2018 06 13 135 140 EN Ali Ramazani Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran Mahdi Tavakolizadeh Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran AND Department of Pharmacognosy, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran Samira Ramazani Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran Hamidreza Kheiri-Manjili Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran Mehdi Eskandari Department of Physiology and Pharmacology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran 2018 05 19 2018 05 19 Background: Development of resistance against the frontline anti-malarial drugs has created an alarming situation, which requires intensive drug discovery to develop new, more effective, affordable and accessible anti-malarial agents. The aim of this study was to assess antiplasmodial activity of the different fractions of root extract of Glycyr­rhiza glabra. Methods: Roots of G. glabra were collected from Tarom district of Zanjan Province in 2016 and then dried root ma­terial was chopped and consecutively extracted by the percolation method using solvents of different polarity. Result­ing extracts were assessed for in vitro and in vivo anti-malarial and cell cytotoxicity activities. Results: Among the three different solvent fractions studied, water-methanol and ethyl acetate fractions showed promising in vitro antiplasmodial activity against CQ-sensitive Plasmodium falciparum 3D7 strain (IC50= 9.95 and 13µg/ml, respectively). Further, the selectivity indices (HeLa cells versus P. falciparum) for the promising water-methanol fraction showed selectivity for P. falciparum and potential safer therapy for human. Interestingly, water-methanol and ethyl acetate fractions showed a significant suppression of parasite growth (72.2% and 65%, respec­tively) in comparison with control group in mice infected with P. berghei (P< 0.05). Conclusion: The promising antiplasmodial activity of the aqueous fraction of G. glabra obtained in our study war­rant bioassay-guided fractionation of this fraction to identify active principles responsible for antiplasmodial activity. https://jad.tums.ac.ir/index.php/jad/article/view/884 https://jad.tums.ac.ir/index.php/jad/article/download/884/381