https://jad.tums.ac.ir/index.php/jad en-US Accepted manuscripts become the permanent property of the Journal and <strong>may not be reproduced, in whole or in part, without the written permission of the Editor</strong> jad@tums.ac.ir (Dr. Hassan Vatandoost) Journals@tums.ac.ir (TUMS Journals Office) Sat, 06 Dec 2025 09:18:12 +0330 OJS 3.1.1.1 http://blogs.law.harvard.edu/tech/rss 60 https://jad.tums.ac.ir/index.php/jad/article/view/1857 <p><strong>Background</strong>: Malaria remains a major global health challenge, disproportionately affecting sub-Saharan Africa. The growing threat of resistance to insecticides in<em> Anopheles</em> vector populations poses a major challenge to the efficacy of core interventions such as long-lasting insecticide-treated nets (LLINs) and indoor residual spraying (IRS). This sys­tematic review aimed to evaluate the contribution of omics approaches, particularly genomics, transcriptomics, and mul­ti-omics, to understanding the resistance mechanisms in malaria vectors in sub-Saharan Africa and their impact on cur­rent and future vector control strategies.</p> <p><strong>Methods: </strong>A comprehensive search was conducted using PubMed, Web of Science, and Google Scholar for eligible studies published between January 2016 and April 2025. Studies using at least one omics approach to investigate re­sistance in <em>Anopheles</em> species were included. We extracted and analyzed data on study location, vector species, omics methods, insecticide classes, resistance mechanisms, and key findings according to PRISMA guidelines.</p> <p><strong>Results</strong>: Twenty-two studies met the inclusion criteria. Genomic and transcriptomic approaches revealed key resistance mechanisms, notably involving metabolic resistance, target-site mutations, and cuticular changes. Multi-omics studies uncovered novel resistance markers such as CYP450 reductase (CPR), UDP-glycosyltransferases (UGTs), and salivary gland proteins. Multi-country collaborations were common, reflecting the cross-border nature of insecticide resistance, while species-specific responses highlighted localized adaptation.</p> <p><strong>Conclusion</strong>: Omics studies have significantly enhanced the understanding of resistance to insecticides among malaria vectors, offering valuable insights for molecular diagnostics and region-specific vector control. Integrating these ap­proaches into routine surveillance is crucial to inform sustainable malaria control and elimination strategies.</p> Yetunde Grace Orogun, Olubanke Olujoke Ogunlana, Damilare Isaiah Taiwo, Olatomide Ayodeji Fadare ##submission.copyrightStatement## https://jad.tums.ac.ir/index.php/jad/article/view/1857 Sat, 06 Dec 2025 09:16:27 +0330