Evaluation of Pyrethroid Susceptibility in Culex pipiens of Northern Izmir Province, Turkey

  • Onur Guntay Section of Molecular Biology, Department of Biology, Faculty of Science, Ege University, Turkey
  • Mehmet Salih Yikilmaz Section of Molecular Biology, Department of Biology, Faculty of Science, Ege University, Turkey
  • Huseyin Ozaydin Section of Molecular Biology, Department of Biology, Faculty of Science, Ege University, Turkey
  • Savas Izzetoglu Section of Molecular Biology, Department of Biology, Faculty of Science, Ege University, Turkey
  • Asli Suner Department of Biostatistics and Medical Informatics, Faculty of Medicine, Ege University, Turkey
Keywords: Bioassay, Insecticide resistance, Pyrethroids, PBO, Culex pipiens

Abstract

Background: Mosquitoes, being a nuisance species, are considered as one of the most important species in public health control programs due to their role as a vector in mosquito-borne diseases observed in humans and animals. We evaluated the susceptibility status of Culex pipiens collected from northern Izmir, Turkey in 2011-16. Methods: Mosquito larvae, collected from three different locations in northern İzmir, were reared in the laboratory. Adult susceptibility bioassays were performed using the WHO insecticide-impregnated papers including deltamethrin 0.05%, permethrin 0.75%, α-cypermethrin 0.05% and cyfluthrin 0.15%. In addition, adult bioassays were performed after the pre-exposure to piperonyl butoxide (PBO) to determine the contribution of P450 detoxification enzymes to the phenotypic resistance. Results: In all of the three populations, high levels of resistance were observed (mortalities<63%) to all of the four pyrethroids. Different pyrethroids but with the same mode of action can exhibit significantly different phenotypic re­sistance in a single population. PBO bioassays also showed that P450 detoxification enzymes can have diverse effects on different pyrethroids. Conclusion: Using just one chemical in a class of insecticide can be misleading for resistance studies.

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Published
2018-10-12
How to Cite
1.
Guntay O, Yikilmaz MS, Ozaydin H, Izzetoglu S, Suner A. Evaluation of Pyrethroid Susceptibility in Culex pipiens of Northern Izmir Province, Turkey. J Arthropod Borne Dis. 12(4):370-377.
Section
Original Article