Molecular Identification of Leishmania Species in Phlebotomus alexandri (Diptera: Psychodidae) in Western Iran

  • Abdollah Naghian Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, Department of Medical Entomology and Vector Control, School of Public Health, Urmyia University of Medical Sciences, Urmyia, Iran
  • Mohammad Ali Oshaghi Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Vahideh Moein-Vaziri Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Yavar Rassi Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Mehdi Sedaghat Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Ehsan Mostafavi Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran
  • Arshad Veysi Zoonoses Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
  • Hassan Soleimani Yazd Health Research Station, National Institute of Health Research, Yazd, Iran
  • Hossein Dehghan Department of Public Health, School of Public Health, Jiroft University of Medical Sciences, Jiroft, Iran
  • Alireza Zahraei-Ramazani Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Hossein Mirhendi Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Esfahan, Iran
  • Mohammad Hassan Amini Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Reza Yaghoobi-Ershadi Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Amir Ahmad Akhavan Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Keywords: Leishmaniasis; Phlebotomus alexandri; Leishmania; Iran

Abstract

Background: Visceral and cutaneous leishmaniasis are common in some areas of Iran and consider as health problems. Phlebotomus alexandri has been incriminated as a suspected vector for the both form of leishmaniasis.Methods: This study was carried out in 4 western provinces of Iran. Sand flies were collected using sticky traps and light traps from indoor and outdoor resting places. Nested PCR was employed to detect Leishmania parasites among collected sand flies.Results: Seven hundred and twenty two P. alexandri females were collected and pooled in 179 batches. Results of nested PCR showed, out of 9 samples from East Azerbaijan Province, only one sample was infected by Leishmania infantum. Of 34 individual and pooled samples from Kermanshah Province, only one pooled sample was infected with Leishmania major and among 30 individual and pooled samples in Fars Province, five specimens were infected by L. major, L. infantum, Leishmania donovani and Leishmania tropica. Furthermore, out of 108 individual and pooled samples from Khuzestan Province, 10 samples showed infection with L. major and L. infantum.Conclusion: The results of this study showed that P. alexandri is more active in hot zones than in moderate zones and this species may be considered as a permissive species.

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Published
2019-08-03
How to Cite
1.
Naghian A, Oshaghi MA, Moein-Vaziri V, Rassi Y, Sedaghat MM, Mostafavi E, Veysi A, Soleimani H, Dehghan H, Zahraei-Ramazani A, Mirhendi H, Amini MH, Yaghoobi-Ershadi MR, Akhavan AA. Molecular Identification of Leishmania Species in Phlebotomus alexandri (Diptera: Psychodidae) in Western Iran. J Arthropod Borne Dis. 14(1):8–16.
Section
Original Article