Effect of Iranian Honey bee (Apis mellifera) Venom on Blood Glucose and In- sulin in Diabetic Rats
AbstractBackground: Diabetes is an important disease. This disease is a metabolic disorder characterized by hyperglycemia resulting from perturbation in insulin secretion, insulin action or both. Honey bee venom contains a wide range of polypeptide agents. The principle components of bee venom are mellitin and phospholipase A2. These components increase insulin secretion from the β-cells of pancreas. This study was conducted to show the hypoglycemic effect of honey bee venom on alloxan induced diabetic male rats.Methods: Eighteen adult male rats weighting 200±20 g were placed into 3 randomly groups: control, alloxan mono- hydrate-induced diabetic rat and treated group that received honey bee venom daily before their nutrition for four months. Forty eight hours after the last injection, blood was collected from their heart, serum was dissented and blood glucose, insulin, triglyceride and total cholesterol were determined.Results: Glucose serum, triglyceride and total cholesterol level in treated group in comparison with diabetic group was significantly decreased (P< 0.01). On the other hand, using bee venom causes increase in insulin serum in com- parison with diabetic group (P< 0.05).Conclusion: Honeybee venom (apitoxin) can be used as therapeutic option to lower blood glucose and lipids in dia- betic rats.
Antia BS, Okokon JE, Okon PA (2005) Hy- poglycemic effect of aqueous leaf ex- tract of Persea Americana Mill on alloxan induced diabetic rats. Indian J Pharmacol. 37: 325 326.
Bomalaski JS, Baker D, Resurreccion NV,Clark MA (1989) Rheumatoid arthritis synovial fluid phospholipase A2 acti- vating protein (PLAP) stimulates hu- man neutrophil degranulation and su- peroxide ion production. J Agents Ac- tions. 27(3–4): 425–427.
Byung-Hyun P, Jin-Woo P (2001) The pro- tective effect of Amomum xanthides extracts against alloxan-induced dia- betic rats through the suppression of NF kB activation. J Exp Med. 33:6468.
Dong JS, Jae Woong L, Young HL, HoSueb S, Chong KL, Jin TH (2007) Therapeutic application of anti-arthri- tis, pain-releasing, and anti-cancer ef- fects of bee venom and its constituent compounds. Pharmacol Ther. 115:246270.
El-Demerdash FM, Yousef MI, Abou El Naga NI (2005) Biochemical study on the hypoglycemic effects of onion and garlic in alloxan- induced diabetic rats.Food Chem Toxicol. 43: 5763.
Frayn KN (1993) Insulin resistance and lipid metabolism. Curr Opin Lipidol. 4:197204.
Fujimoto WY, Metz SA (1987) Phasic effects of glucose, phospholipase A2, and lysophospholipids on insulin secretion. J Endocrinol. 120(5): 1750.
Gauldie J, Hanson JM, Rumjanek FD, Shipolini RA, Vernon ChA (1976) The peptide components of beevenom. Eur J Biochem. 61: 3693761.
Habermann E (1972) Bee and wasp venoms. Science (Washington DC).177(3): 14–322.
Jong-Yeon Kim, Song-Hyun Cho, Yong- Woon Kim, Eung-Chan Jang, So-Yung Park, Eun- Jung Kim, Suck-Kang Lee (1999) Effect of BCG,Lymphotoxin and Bee Venom on insulities and De- velopment of IDDM in Non-Obese Di- abetic Mice. J Korean Med Sci. 14:648–652.
Smith JF (2003) Antidiabetic drugs. J Med Lib. 5: 56.
Kim JY, cho SH, kim YW, Jang EC, Park SY, Kim EJ, Lee SK (1999) Effects of BCG, lymphotoxin and bee venom on insulitis and development of IDDM in non-obese diabetic mice. J Korean Med Sci. 14(6): 648652.
Lee JD, Park HJ, Chae Y, Lin S, (2005) An overview of Bee Venom acupuncture in the treatment of arthritis. Evid Based Complement Alternat Med. 2: 79–84.
Mazzanti C, Spanevello R, Ahmed M, Schmatz R, Mazzanti A, Salbego F, Grac D, Sallis E, Morsch V, Schetinger M (2007) Cyclosporine inhibits acetyl cho- linesterase activity in rats experimentally demyelinated with ethidium bromide. Int
J Devl Neuroscience. 25: 259264. Mirshafiey A (2007) Venom therapy in multiple sclerosis. Neuropharmacology. 53:353–361.
Morgan NG, Montague w (1984) Stimula- tion of insulin secretion from isolated rat islets of Langerhans by melittin. J Biosci Rep. 4(8): 665671.
Nuraliev IN, Avezov GA (1992) The efficacy ofquercetin in alloxan diabetes. Eksp Klin Farmakol. 55: 4244.
Pedarzani P, Stocker M (2008) Molecular and cellular basis of small and inter- mediate conductance,calcium activated potassium channel function in the brain. Cell Mol Sci. 65: 3196–3217.
Seyed Khoei N, Atashpaz S, Ghabili K, Seyed Khoei N, Omidi Y (2009) Melittin and hyaluronidase compound derived from Bee venom for the treatment of multiple sclerosis. Iran J Med Hypotheses Ideas.9(3): 24.
Simonsson E, Karlsson S, Ahren B (2000) Islet phospholipase A(2) activation is po- tentiated in insulin resistant mice. Biochem Biophys Res Communi. 272(2):539543.
Son DJ, Lee JW, Lee YH, Song HS, Lee CK,Hong JT (2007) Therapeutic application of anti-arthrities, pain-releasing, and anti- cancer effect of Bee Venom and its com- pounds. Pharmacol Ther. 115: 246–270.
Soto C, del Razo LM, Neri L (2001) Alloxan decreases intracellular potas- sium content of the iolated frog skin epithelium. Comp Biochem Physiol C Toxico Pharmacol. 130(1): 1927.
Reinner E, Bjorkhem I, Angelin B, Ewerth S,Einarsson K (1989) Bile acid synthesis in humans: regulation of hepatic micro- somal cholesterol 7 alpha-hydroxylase activity. J Gastroenterol. 97 : 14981505.
Viana GS, Medeiros AC, Lacerda AM, Leal LK, Vale TG, Matos FJ (2004) Hypo- glycemic and anti-lipidemic effects of the aqueous extract of Cissus si-cyoides.
J BMC Pharmacol. 8: 4 9.Wesselius T, Heersema DJ, Mostert JP,Heerings M, Admiraai-Behloul F, Talebian A,Van Buchen MA, De Key- ser J (2005) A randomized crossover study of Bee sting therapy for multiple sclerosis. Neurology. 65: 1764–1768.
Williams G, Pickup JC (2000) Handbook of Diabetes (2nd edition) Blackwell Sci- ence, Oxford. Yadav UC, Moorthy K, Baquer NZ (2004) Effects of sodium orthovanadate and Trigonella foenum- graecum seeds on hepatic and renal lipogenic enzymes and lipid profile dur- ing alloxan diabetes. J Biosci. 29(1):8191.
Zalat S, Nabil Z, Hussein A, Rakha M (1999) Biochemical and haematologi- cal studies of some solitary and social bee venoms. Egypt J Biology. 1: 577.
Zhang X F, Tan BKH (2003) Effects of an ethanolic extract of Gynura procumbens on serum glucose, cholesterol and triglyc- eride levels in normal and streptozotocin- induced diabetic rats. Singapore Med J.41(1): 16.