Biocontrol of the Brown-banded Cockroach, Supella longipalpa F. (Blattaria: Blattellidae), with Entomopathogenic Fungus, Metharhizium anisopliae
AbstractBackground: Considering to the high distribution of cockroaches as urban pests, the efficacy of different formulations of Metarhizium anisopliae strain Iran 437C were assessed against the brown-banded cockroach, Supella longipalpa F. under laboratory and field conditions.Methods: Metarhizium anisopliae isolates were screened with immersing adults of the brown-banded cockroachs in aqueous suspension of 108 conidia ml-1 followed by surface or bait treated with different doses of the most virulent isolate against the nymphs. Then formulations of conidia oil-in-water were examined versus cockroach nymphs using different plant oils and paraffin. Then they were evaluated and compared with aqueous suspension and control group. On a large-scale, the sunflower oil-in-water formulation of conidia was sprayed at houses using a hand sprayer.Results: Metarhizium anisopliae IRAN 437C was the most virulent isolate against the brown-banded cockroach, causing 100% mortality in adults at seven days post-exposure. Inoculated bait with this isolate was not enough pathogenic against the cockroach even at two weeks after treatment. Treated surface with conidia as aqueous suspension or oil-in- water formulation was more effective than the bait formulation against the cockroach caused 39.4–97.2% mortality compared with 2.5% mortality in control group after two days. Spraying the conidia formulated with sunflower oil was an effective formulation causing 76.1% reduction in the cockroach density on the third day post treatment in the houses.Conclusion: The oil-in-water formulation of M. anisopliae IRAN 437C could be recommended as a promising alternative for cockroach control.
Bischoff JF, Rehner SA, Humber RA (2009) A multilocus phylogeny of the Me- tarhizium anisopliae lineage. Myco- logia. 101(4): 512–530.
Butt TM, Goettel MS (2007) Bioassays of Entomopathogenic Fungi. In: Navon A, Ascher KRS (Eds): Bioassay of Microbes and Nematodes,. CABI pub- lishing, Wallingford, Oxfordshire, UK, pp. 141–195
Cochran DG (1999) Cockroaches, Their Bi- ology, Distribution and Control. Swit- zerland World Health Organization. WHO/CDS/CPS/WHOPES/ 99.3. p.83.
Eggleston PA, Arruda LK (2001) Ecology and elimination of cockroaches and allergens in the home. J Allergy Clin Immunol. 107: 422–429.
Ehdae B (2015) StIsolation and identifica- tion bacteria from cockroaches of Ahvaz (sw of Iran) hospitals and de- termination of their susceptibility to antibiotics. [Doctorate dissertation]. School of Pharmacy, Ahvaz Jun- dishapur University of Medical Sci- ences, Ahvaz. Iran.
Gunner HB, Agudelo-Silva F, Johnson CA (1991) Method and Device for the Biological Control of Cockroaches. United States Patent No.5.057.315.
Freimoser FM, Screen S, Bagga S, Hu G, St.Leger RJ (2003) EST analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect hosts. Microbiology. 149(1): 239–247.
Kaakeh W, Reid BL, Bennett GW (1996)Horizontal transmission of the ento- mopathogenic fungus Metarhizium anisopliae and hydromethylnon among German cockroaches (Dictyoptera: Blattellidae). J Entomol Sci. 31: 378–390.
Kassa A (2003) Development and testing of mycoinsecticides based on subimerged spores and aerial conidia of the en- tomopathogenic fungi Beauveria bas- siana and Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) for control of locusts, grasshoppers and storage pests. [Ph.D Thesis]. Gottin- gen University, Germany.
Lacey LA, Frutos R, Kaya HK, Vail P (2001) Insect pathogens as biological control agents: Do they have a fu- ture? Biol Control. 21: 230–248.
Ladonni H, Paksa A, Nasirian H, Doroudgar A, Abaie MR (2013) Detection of Carbamat and organo phosphorus susceptibility levels in German cock- roach in vivo. Toloo e Behdasht.40(3): 95–105.
Lee CY, Yap HH, Chong NL (1996) Com- parison of selected biological param- eters of laboratory susceptible and field collected strains of the German cockroach, Blattella germanica (L.) (Dictyoptera: Blattellidae). Malaysian J Science. 17A: 37–48.
Le Guyader A, Rivault C, Chaperon J (1989) Microbial organisms carried by the brown-banded cockroaches in rela- tion to their spatial distribution in a hospital. Epidem Inf. 102: 485–492.
Lopes RV, Alves SB (2011) Differential Susceptibility of Adults and Nymphs of Blattella germanica (L.) (Blat- todea: Blattellidae) to Infection by Metarhizium anisopliae and Assess- ment of Delivery Strategies. Neotrop J Entomol. 40(3): 368–374.
Mulla MS, Norland L, Fanara DM, Darwazeh HA, McKean DW (1971) Control of Chironomid midges inrecreational lakes. J Economic Entomol. 64: 300–307.
Nasirian H, Ladonni H, Shayeghi M, Vatan- doost H, Yaghoobi-Ershadi MR, Rassi Y, Abolhassani M, Abaei MR (2006) Comparison of permethrin and fipronil toxicity against German cockroach (Dictyoptera: Blattellidae) strains, Iran J Public Health. 35(1): 63–67.
Pachamuthu P, Kamble ST (2000) In vivo study on combined toxicity of Me- tarhizium anisopliae (Deuteromy- cotina: Hyphomycetes) strain ESC-1 sublethal doses of chlorpyrifos, prope- tamphos, and cyfluthrin against Ger- man cockroach (Dictyoptera: Blattelli- dae). J Econ Entomol. 93(1): 60–70.
Peairs FB (2014) Cockroaches, Colorado State University Extension bioagri- cultural sciences and pest management.
Quesada-Moraga E, Santos-Quiro's R, Valverde-Garcı'a P, Santiago-A'lva- rez C (2004) Virulence, horizontal transmission, and sublethal reproduc- tive effects of Metarhizium anisopli- ae (Anamorphic fungi) on the Ger- man cockroach (Blattodea: Blattelli- dae). J Pathology. (87): 51–58.
Rivault C, Cloarec A, Mathieu N, Blanc N (1994) Les blattes, un animal de ville a toutes saisons. Les Ann. de la Re- cherche Urbaine. 61: 33–38.
Rozendal JA (1997) Vector Control: meth- ods for use by individuals and com- munities. World Health Organiza- tion. AITBS Publisherand Disribu- tors (Regd.) Delhi.
Savoldelli S, Luciano S (2005) Laboratory evaluation of Insecticide Gel Baits for Control of Supella longipalpa (Dictyoptera: Blattellidae). Proceeding of Fifth International Conference on Urban Pests, Malaysia, pp. 156–158.
Sharififard M, Mossadegh MS, Vazirianza- deh B and Latifi SM (2013) Evalua- tion of Conidia Dust Formulation of Metarhizium anisopliae to Biocon- trol the Brown-Banded Cockroach, Supella longipalpa F. Jundishapur J Microbiol. 2014 June. 7(6): e10721.
Sharififard M, Mossadegh MS, Vazirianza- dhe B, ZareiMahmoudabadi A (2011a) Laboratory pathogenicity of Ento- mopathogenic Fungi, Beauveria bas- siana (Bals.) Vuill.and Metarhizium anisoplae (Metch.) Sorok.to larvae and adult of house fly, Musca domes- tica L. (Diptera: Muscidae). Asian J Biol Sci. 4(2): 128–137.
Sharififard M, Mossadegh MS, Vazirianza- dhe B, ZareiMahmoudabadi A (2011b) Interactions among entomopathogen- ic fungus, Metarhizium anisopliae (Metch.) Sorok and sublethal doses of spinosad in the control of house fly, Musca domestica L. Iran J Ar- thropod-Borne Dis. 5(1): 28–36.
Sharififard M, Mossadegh MS, Vazirianza- dhe B (2012) Effects of Temperature and Humidity on the pathogenicity of Entomopathogenic Fungi in Control of the House fly, Musca domestica L. (Diptera: Muscidae) under laboratory conditions. J Entomol. 9(5): 282–288.
Stankus RP, Horner WE, Lehrer SB (1990) Identification and characterization of important cockroach allergens. J Al- lergy Clin Immunol. 86(5): 781–787.
Talwar BH (2005) Isolation and Characteri- zation of Entomopathogenic fungi and their effectiveness. [PhD The- sis], Department of agricultural Mi- crobiology, University of Agricul- tural Sciences, Dharwad, India.
Thavara U, Tawatsin A, Bhakdeenuan P, Wongsinkongman P, Boonruad T, Bansiddhi J, Chavalittumrong P, Komalamisra N, Siriyasatien P, Mul- la MS (2007) Repellent activity of essential oils against cockroaches (Dictyoptera: Blattidae, Blattellidae, and Blaberidae) in Thailand. South- east Asian J Trop Med Public Health.38(4): 663–673.
Vazirianzadeh B, Dehghani R, Mehdinejad M, Sharififard M, Nasirabadi N (2014) The first report of drug resistant bacte- ria isolated from the brown-banded cockroach, Supella longipalpa, in Ah- vaz, South-western Iran. J Arthropod- Borne Dis. 8(1): 53–59.
Zimmermann G (1993) The entomopatho- genic fungus, Metarhzium anisopliae and its potential as a biological agent. Pestic Sci (37): 375–379.
Zurek L, Wes Watson D, Schal C (2002) Synergism between Metarhizium an- isopliae (Deuteromycota: Hyphomy- cetes) and boric acid against the Ger- man cockroach (Dictyoptera: Blatelli- dae). J Biol Control. 23: 296–302.