Journal of Arthropod-Borne Diseases 2014. 8(2):147-155.

Chemical Composition, Larvicidal and Repellency Properties of Cionura erecta (L.) Griseb. Against Malaria Vector, Anopheles stephensi Liston (Diptera: Culicidae).
Ehssan Mozaffari, Mohammad Reza Abai, Mahnaz Khanavi, Hassan Vatandoost, Mohammad Mehdi Sedaghat, Abbas Moridnia, Mahsa Saber-Navaei, Alireza Sanei-Dehkordi, Fatemeh Rafi

Abstract


Background: Application of plant derivatives have been suggested as alternative sources for mosquito control.
Methods: The root essential oil and methanol extract of Cionura erecta (L.) Griseb was tested under laboratory conditions for larvicidal and skin repelleny activities against Anopheles stephensi. The chemical compositions of essential oils were analyzed using gas chromatography- mass spectrometry.
Results: Among the five concentrations tested, the 320 ppm of essential oil and 1280 ppm of methanolic extract had the most toxic effects yielding 100% mortality. The LC50 values of C. erecta for both essential oil and methanolic extract were 77.30 and 250.38 ppm, respectively. A total of 19 compounds were identified in essential oil of root. The major components were detected in root essential oil including Cedren-9-one (7.89%), alpha cadinol (5.67%), eugenol (4.02%) and alpha muurolene (3.58%). The protection time of 50% solution of essential oil against bites of An. stephensi was 2.28 hour on white rabbit and the ED50 and ED90 values of the essential oil were 10.12 and 23.01 ppm respectively.
Conclusion: The findings suggest that C. erecta oil has a potential source as larvicidal and repellency properties against An.stephensi.


Keywords


Anopheles stephensi; Cionura erecta; Essential Oil; Extract; Larvicidal Effect; Repellency

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References


Adams RP (2001) Identification of essential oil components by gas chromatography/ mass spectroscopy. Allured, Carol Stream IL, USA.

Ansari MA, Vasudevan P, Tandon M, Razdan RK (2000) Larvicidal and mosquito re- pellent action of peppermint (Mentha piperita) oil. Bioresour Technol. 71:267–271.

Aktar MDW, Sengupta D, Chowdhury A (2009) Impact of pesticides use in agriculture: their benefits and hazards. Interdiscip Toxicol. 2(1): 1–12.

ASTM (2000) Standard test methods for laboratory testing of non-commercial mosquito repellent formulation on the skin. American Society for Testing and Materials, ASTM-E951-94 (Reapproved 2000).

Fradin MS (1998) Mosquitoes and mosquito repellents. Ann of Internal Med. 128:931–940.

Ghosh A, Chowdhury N, Chandra G (2012) Plant extracts as potential mosquito larvicides. Indian J Med Res. 135(5): 581–598.

Govindarajan M, Mathivanan T, Elumalai K, Krishnappa K, Anandan A (2011) Ovi- cidal and repellent activities of botan- ical extracts against Culex quinque- fasciatus, Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). Asian Pac J Trop Biomed. 1: 43–48.

Hadjiakhoondi A, Vatandoost H, Abousaber M, Khanavi M, Abdi L (2000) Chemi- cal composition of the essential oil of Tagetes minuta L. and its effects on Anopheles stephensi larvae in Iran. J Med Plants. 7: 33–100.

Hadjiakhoondi A, Vatandoost H, Jamshidi A, Bagherj Amiri E (2003) Chemical con- stituents and efficacy of Cymbopogon olivieri (Boiss) bar essential oil against malaria vector, Anopheles stephensi. Daru. 11: 125–128.

Hadjiakhoondi A, Vatandoost H, Khanavi M, Abai MR (2005) Biochemical investiga- tion of different extracts and larvicidal activity of Tagetes minuta L on Anoph- eles stephensi larvae. Iran J Pharm Sci.1: 81–84.

Isman MB (2000) Plant essential oils for pest and disease management. Crop Prot.19: 603–608.

Isman MB (2006) Botanical insecticides, de terrents, and repellents in modern agri- culture and an increasingly regulated world. Annu Rev Entomol. 51: 45–66.

Katz TM, Miller JH, Hebert AA (2008) Insect repellents: historical perspectives and new developments. J Am Acad Der- matol. 58: 865–871.

Khanavi M, Vatandoost H, Khosravi-Dehaghi N, Sanei-Dehkordi A, Sedaghat MM, Hadjiakhoondi A, Hadjiakhoondi F (2013) Larvicidal activities of some Iranian native plants against the main malaria vector, Anopheles stephensi. Acta Med Iran. 51: 141–147.

Klun JA, Khrimian A, Debboun M (2006) Re- pellent and deterrent effects of SS220, Picaridin, and Deet suppress human blood feeding by Aedes aegypti, Anoph- eles stephensi, and Phlebotomus papa- tasi. J Med Entomol. 43: 34–39.

Kunz SE, Kemp DH (1994) Insecticides and acaricides: resistance and environmen- tal impact. Rev Sci Tech. 13: 1249.

Lehane MJ (1991) Biology of blood-sucking insects. London, UK: Harper Collins Academic.

Mullai K, Jebanesan A, Pushpanathan T (2008) Mosquitocidal and repellent activity of the leaf extract of Citrullus vulgaris (cu- curbitaceae) against the malarial vec- tor, Anopheles stephensi liston (diptera culicidae). Eur Rev Med Pharmacol Sci.12: 1–7.

Myrianthopoulos V, Fokialakis N, Melliou E, Mitaku S (2007) Chemical composi- tion of essential oil of Cionura Erecta (Asclepiadaceae) Inforescences. J Essent Oil Res. 19: 266–268.

Nerio LS, Olivero-Verbel J, Stashenko E (2010) Repellent activity of essential oils: a review. Bioresour Technol. 101:372–378.

Oshaghi MA, Ghalandari R, Vatandoost H, Shaeghi M, Kmali-Nejad M, Tourabi- Khaledi H, Abolhassani M, Hashem- zadeh M (2003) Repellent effect of extracts and essential oil of Citrus limon (Rutaceae) and Melissa officinalis (Labiatae) against main malaria vector, Anopheles stephensi (Diptera: Culicidae) in Iran. Iran J Public Health. 32: 47–52.

Pandey SK, Upadhyay S, Tripathi AK (2009) Insecticidal and repellent activities of thymol from the essential oil of Trachy- spermum ammi (Linn) Sprague seeds against Anopheles stephensi. Parasitol Res. 105: 507–512.

Prabhu K, Murugan K, Nareshkumar A, Ramasubramanian N, Bragadeeswaran S (2011) Larvicidal and repellent po- tential of Moringa oleifera against ma- larial vector, Anopheles stephensi Lis- ton (Insecta: Diptera: Culicidae). Asian Pac J Trop Biomed. 1: 124–129.

Prajapati V, Tripathi AK, Aggarwal KK, Khanuja SPS (2005) Insecticidal, re- pellent and oviposition-deterrent activ- ity of selected essential oils against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. Bioresour Tech- nol. 96: 1749–1757.

Rajkumar S, Jebanesan A (2007) Repellent activity of selected plant essential oils against the malarial fever mosquito Anopheles stephensi. Trop Biomed. 24:71–75.

Sedaghat MM, Sanei-Dehkordi A, Abai MR, Khanavi M, Mohtarami F, Saim abadi Y, Vatandoost H (2011a) Larvicidal Activity of Essential Oils of Apiaceae Plants against Malaria Vector, Anoph- eles stephensi. Iran J Arthropod-Borne Dis. 5(2): 51–59.

Sedaghat MM, Sanei-Dehkordi A, Khanavi M, Abai MR, Hadjiakhoondi A, Mohtarami F, Vatandoost H (2010) Phytochemistry and larvicidal activity of Eucalyptus camaldulensis against malaria vector, Anopheles stephensi. Asian Pac J Trop med. 3: 841–845.

Sedaghat MM, Sanei-Dehkordi A, Khanavi M, Abai MR, Mohtarami F, Vatandoost H (2011b) Chemical composi- tion and larvicidal activity of essential oil of Cupressus arizonica E.L. Greene against malaria vector Anopheles ste- phensi Liston (Diptera: Culicidae). Phar- macognosy Res. 2: 135–139.

Tavassoli M, Shayeghi M, Vatandoost H, Khoobdel M, Salari M, Ghaderi A, Rafi F (2011) Repellency Effects of Essen- tial Oils of Myrtle (Myrtus communis), Marigold (Calendula officinalis) Com- pared with DEET against Anopheles stephensi on Human Volunteers. Iran J Arthropod-Borne Dis. 5: 10–22.

Vatandoost H, Hanafi-Bojd AA (2005) Current resistant status of Anopheles stephensi Liston to different larvicides in Hor- mozgan Province, southeastern Iran. Pak J Biol Sci. 8: 1568–1570.

Vatandoost H, Hanafi-Bojd AA (2008) La- boratory evaluation of 3 repellents against Anopheles stephensi in the Is- lamic Republic of Iran. East Mediterr Health J. 14: 260–267.

Vatandoost H, Mashayekhi M, Abai MR, Aflatoonian MR, Hanafi-Bojd AA, Sharifi I (2005) Monitoring of insecti- cides resistance in main malaria vectors in a malarious area of Kahnooj district, Kerman Province, southeastern Iran. J Vector Borne Dis. 42: 100–108.

Vatandoost H, Sanei Dehkordi A, Sadeghi SMT, Davari B, Karimian F, Abai MR, Sedaghat MM (2012) Identification of chemical constituents and Larvicidal activity of Kelussia odoratissima Mozaffarian essential oil against two mosquito vectors Anopheles stephensi and Culex pipiens (Diptera: Culicidae). Exp Parasitol. 132: 470–474.

Vatandoost H, Vaziri VM (2004) Larvicidal activity of a neem tree extract (Neemarin) against mosquito larvae in the Islamic Republic of Iran. East Mediterr Health J. 10: 573–581.

Vatandoost H, Zahirnia AH (2010) Responsiveness of Anopheles maculipennis to different imagicides during resurgent malaria. Asian Pacific J Trop Med. 3:360–363.

WHO (1981) Instruction for determining the susceptibility or resistance of mosquito larvae to insecticides. World Health Organization-VBC.

WHO (2012) World Malaria Report. World Health Organization.

Yaghoobi-Ershadi MR, Akhavan AA, Jahani- fard E, Vatandoost H, Amin GH, Moosavi L, Ramazani AR, Abdoli H, Arandian MH (2006) Repellency effect of Myrtle essential oil and DEET against Phlebo- tomus papatasi, under Laboratory Con- ditions. Iran J Public Health. 35: 7–13.


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