Wolbachia Endobacteria in Natural Populations of Culex pipiens of Iran and its Phylogenetic Congruence

  • Mohsen Karami Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Seyed Hassan Moosa-Kazemi Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Ali Oshaghi Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Hasan Vatandoost Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Mehdi Sedaghat Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Ramazan Rajabnia Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran
  • Mostafa Hosseini Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Naseh Maleki-Ravasan Malaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  • Yousef Yahyapour Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran
  • Elaheh Ferdosi-Shahandashti Department of Advanced Technologies in Medicine (SATiM), Medical Biotechnology,Tehran University of Medical Sciences, Tehran, Iran
Keywords: Culex pipiens, Wolbachia, cytoplasmic incompatibility, nested-PCR, Iran

Abstract

Background: Wolbachia are common intracellular bacteria that infect different groups of arthropods including mos­quitoes. These bacteria modify host biology and may induce feminization, parthenogenesis, male killing and cyto­plasmic incompatibility (CI). Recently Wolbachia is being nominated as a bio-agent and paratransgenic candidate to control mosquito borne diseases.Methods: Here we report the results of a survey for presence, frequency, and phylogenetic congruence of these en­dosymbiont bacteria in Culex pipiens populations in Northern, Central, and Southern parts of Iran using nested-PCR amplification of wsp gene.Results: Wolbachia DNA were found in 227 (87.3%) out of 260 wild-caught mosquitoes. The rate of infection in adult females ranged from 61.5% to 100%, while in males were from 80% to 100%. The Blast search and phyloge­netic analysis of the wsp gene sequence revealed that the Wolbachia strain from Iranian Cx. pipiens was identical to the Wolbachia strains of supergroup B previously reported in members of the Cx. pipiens complex. They had also identical sequence homology with the Wolbachia strains from a group of distinct arthropods including lepidopteran, wasps, flies, damselfly, thrips, and mites from remote geographical areas of the world.Conclusion: It is suggested that Wolbachia strains horizontally transfer between unrelated host organisms over evo­lutionary time. Also results of this study indicates that Wolbachia infections were highly prevalent infecting all Cx. pipiens populations throughout the country, however further study needs to define Wolbachia inter-population repro­ductive incompatibility pattern and its usefulness as a bio-agent control measure.

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Published
2016-06-18
How to Cite
1.
Karami M, Moosa-Kazemi SH, Oshaghi MA, Vatandoost H, Sedaghat MM, Rajabnia R, Hosseini M, Maleki-Ravasan N, Yahyapour Y, Ferdosi-Shahandashti E. Wolbachia Endobacteria in Natural Populations of Culex pipiens of Iran and its Phylogenetic Congruence. jad. 10(3):349-65.
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