Journal of Arthropod-Borne Diseases 2016. 10(3):405-414.

Detection of Bendiocarb and Carbaryl Resistance Mechanisms among German Cockroach Blattella germanica (Blattaria: Blattellidae) Collected from Tabriz Hospitals, East Azerbaijan Province, Iran in 2013
Afshin Salehi, Hassan Vatandoost, Teimour Hazratian, Alireza Sanei-Dehkordi, Hossein Hooshyar, Mohsen Arbabi, Yaser Salim-Abadi, Reza Sharafati-Chaleshtori, Mohammad Amin Gorouhi, Azim Paksa


Background: Insecticide resistance is one of the serious problems for German cockroach control program. This study was conducted to determine the bendiocarb and Carbaryl resistance mechanisms in German cockroaches using the piperonyl butoxide (PBO).
Methods: Bioassay tests were conducted with 4 to 6 different concentrations of both insecticides with four replicates of 10 susceptible strain cockroaches per concentration to determine of discriminative concentration. After determining discriminative concentration, the result was compared to wild strain. The levels of susceptibility and resistance ratio (RR) and synergism ratio (SR) were calculated for each five wild strains. Moreover resistance mechanisms in wild strains were determined using PBO synergist in vivo.
Results: Hospital strains showed different levels of resistance to bendiocarb and carbaryl compared to susceptible strain. The bendiocarb and carbaryl resistance ratios ranged from 2.11 to 7.97 and 1.67 to 2 at LD50 levels, respec­tively. The synergist PBO significantly enhanced the toxicity of bendiocarb and carbaryl to all strains with different degrees of synergist ratio, 1.31, 1.39, 3.61, 1.78, 1.62 and 2.1 fold for bendiocarb, 1.19, 1.18, 1.12. 1.29, 1.45 and 1.11- fold for carbaryl, suggesting monooxygenase involvement in bendiocarb and carbaryl resistance.
Conclusion: The synergetic effect of PBO had the highest effect on bendiocarb and resistance level was significantly reduced, which indicates the important role of monoxidase enzyme in creating resistance to Bendiocarb. Piperonyl butoxide did not have a significant synergistic effect on carbaryl and did not significantly break the resistance.


German cockroach; Resistance mechanism; Carbaryl; Bendiocarb

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Ahad M, Hollingworth RM (2004) Synergist of insecticide provides evidence of metabolic mechanisms of resistance in the oblique-banded leaf roller Choris- toneura rosaceana. Pest Manag Sci.60: 465–473.

Alves AP, Allgeier WJ, Siegfried BD (2008) Effects of the synergist S, S, S-tributyl phosphorotrithioate on indoxacarb tox- icity and metabolism in the European corn borer, Ostrinia nubilalis (Hübner). Pest Biochem Physiol. 90(1): 26–30.

Behmanesh F, Shoja M, Khajedaluee M (2010) Prevalence of aeroallergens in childhood asthma in Mashhad. Maced J Med Sci. 3(3): 295–298.

Chai RY, Lee CY (2010) Insecticide re- sistance profiles and synergism in field population of the German cockroach (Dictyoptera: Blattellidae) from Singa- pore. J Econ Entomol. 103: 460–471.

Scot GG, Cochran DC, Siegfried B (1990) Insecticide toxicity, synergism, and re- sistance in the German cockroach (Dic- tyoptera: Blattellidae). J Econ Ento- mol. 83(5): 1698–1703.

Dong k, Scott JG (1992) Synergism of chlorpyrifos against the German cock- roach, Blattella germanica. Med Vet Entomol. 6(3): 241–243.

El-Merhibi A, Kumar A, Smeaton T (2004) Role of piperonyl butoxide in the tox- icity of chlorpyrifos to Ceriodaphnia dubia and Xenopus laevis. Ecotoxicol Environ Saf. 57(2): 202–212.

Fahiminia M, Paksa A, Zarei A (2010) Sur- vey of optimal methods for the control of cockroaches in sewers of Qom City. Iran J Health and Environ. 3(1): 19–26.

Fakoorziba MR, Eghbal F, Hassanzadeh J, Moemenbellah-Fard MD (2010) Cock- roaches (Periplaneta Americana and Blattella germanica) as potential vec- tors of the pathogenic bacteria found in nosocomial infections. Ann Trop Med Parasitol.104 (6): 521–528.

Finny DJ (1972) Probit analysis. Third ed, Cambridge University, London.

Hemingway J, Small GJ, Monro AG (1993) Possible mechanisms of organophos- phorus and carbamate insecticide re- sistance in German cockroaches (Dic- tyoptera: Blattelidae) from different Ge- ographical areas. J Econ Entomol. 86 (6): 1623–1630.

Hodgson E, Levi PE (1998) Interactions of Piperonyl Butoxide with Cytochrome P450. In: Jones DG (Ed) Piperonyl Butoxide: The Insecticide Synergist. Academic Press, San Diego, CA, pp.41–53.

Ladonni H (1993) Susceptibility of Blattella germanica to different insecticides in different hospitals in Tehran, Iran. J Entomol Soc Iran. 12 and 13: 23–28.

Ladonni H (1997) Susceptibility of different field strains of Blattella germanica to four pyrethroids (Orthoptera: Blattelli- dae). Iran J Publ Health. 26: 35–40.

Ladonni H, Sadegheyani S (1998) Perme- thrin toxicity and synergistic effect of piperonyl butoxide in the first nymphal stage of Blattella germanica (Dictyop- tera: Blattellidae). Iran J Publ Health.27: 44–50.

Lee CY, Lee LC, Ang BH, Chong NL (1999) Insecticide resistance in Blat- tella germanica (L.) (Dictyoptera: Blat- tellidae) from hotel and restaurant in Malaysia. J Econ Entomol. 103(2):460–471.

Lee CY, Yap HH, Chong NL, Lee RST (1996) Insecticide resistance and syn- ergism in field collected German cock- roach (Dictyoptera: Blattelllidae) in Pen- insular Malaysia. Bull Entomol Res.86: 675–682.

Limoee M, Enayati AA, Khassi K, Salimi M, Ladonni H (2011) Insecticide re- sistance and synergism of three field collected strains of the German cock- roach Blattella germanica (L.) (Dicty- optera: lattellidae) from hospitals in Kermanshah, Iran. Tropical Bio-Med.28(1): 111–118.

Limoee M, Ladonni H, Enayati AA, Vatan- doost H, Aboulhasani M (2006) De- tection of pyrethroid resistance and cross- resistance to DDT in seven field collected strains of the German cock- roach Blattella germanica (L.) (Dicty- optera: Blattellidae). J Bio Sc. 6(2):382–387.

Metcalf RL (1967) Mode of action insecti- cide resistance. Annu Rev Entomol.12: 229–256.

Nasirian H (2007) Duration of fipronil and imidacloprid gel baits toxicity against Blattella germanica strains of Iran. Iran J Arthropod-Borne Dis. 1: 40–47.

Nasirian H (2010) An overview of German cockroach, Blattella germanica, stud- ies conducted in Iran. J Bio Sc. 13(22):1077–1084.

Nasirian H, Ladonni H, Aboulhassani M, Limoee M (2006) Susceptibility of field populations of Blattella german- ica (Blattaria: Blattellidae) to spi- nosad. Pak J Biol Sci. 14: 862–868.

Nejati J, Keyhani A, Moosa-Kazemi SH, Mohammadi M (2012) cockroaches’ bacterial infections in wards of hospi- tals, Hamedan City, west of Iran. Asian Pac J Trop Dis. 2(5): 381–384.

Paksa A, Doroudgar A, Vatandoost H (2014) Detection of cyfluthrin resistance mech- anisms among German cockroach strains in vivo in Kashan. J Feyz. 17(6): 590–596. (in Persian).

Paksa A, Ladonni H, Nasirian H (2011) De- tection of malathion and chlorpyrifos resistance mechanism in German cock- roaches (Blattella germanica, Insecta: Blattodea: Blattellidae) using piperonyl butoxide and tributyl phosphorotrithi- oate. Iran J Publ Health. 15(3): 243–253.

Payne GT, Brown TM (1984) EPN and S, S, S-tributyl phosphorotrithioate as syn- ergists of methyl parathion in resistant tobacco budworm larvae (Lepidoptera: Noctuidae). J Econ Entomol. 77: 294–297.

Reirson DA, Rust MK, Slater AG, Slater TAM (1998) Insecticide resistance af- fects cockroach control. California Agr.42: 18–20.

Rust MK (1987) Comparison of the labora- tory and field efficacy of insecticide used for German cockroaches control. J Econ Entomol. 71: 704–707.

Scharft ME, Bennett GW, Reid BL, Qui C (1995) Comparisons of three insecti- cide resistance detection methods for the German cockroach (Dictyoptera: Blattellidae). J Econ Entomol. 88: 536–542.

Scott JG, Cochran DG, Siegfried BD (1990) Insecticide toxicity, synergism, and re- sistance in the German cockroach (Dic- tyoptera: Blattellidae). J Econ Ento- mol. 83: 1698–1703.

Shinji Kasai S, Weerashinghe IS, Shono T (1998) P450 monooxygenases are an important mechanism of permethrin re- sistance in Culex quinquefasciatus Say larvae. Arch Insect Biochem Physiol.

: 47–56.

Talebi Jahromi K (2006) Pesticides toxicol- ogy. First ed. Tehran University Press, Tehran.

Tozzi A (1998) A brief history of the devel- opment of piperonyl butoxide as an insecticide Synergist. In: Jones DG (Ed) Piperonyl Butoxid: The Insecticide Syn- ergist, Academic Press, San Diego, CA, pp. 1–5.

Valles SM, Yu SJ, Koehler PG (1994) Detoxifying enzymes in adults and nymphs of the german cockroach: evidence for different microsomal monooxygenase systems. Pestic Biochem Physiol. 49:183–190.


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