Identification of Hymenolepis diminuta Cysticercoid Larvae in Tribolium castaneum (Coleoptera:Tenebrionidae) Beetles from Iran

  • Gholamreza Mowlavi Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Farideh Shahbazi Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Reza Abai Department of Medical Entomology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Faezeh Najafi Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Bibi Razieh Hosseini-Farash Department of Parasitology and Mycology, Research Center for Skin Diseases and Cutaneous Leishmani¬asis, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  • Salma Teimoori Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Centre of Excellence for Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Bangkok, Thailand
  • Hamid Hasanpour Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Saied Reza Naddaf Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
Keywords: Hymenolepis diminuta, Cysticercoid, Tribolium castaneum, Iran

Abstract

Background: Hymenolepis diminuta is a cestod of rodents and rarely infects humans. Infection in humans is via ingestion of infected insects. This study was aimed to detect H. diminuta cysticercoids in red flour beetles, Tribolium castaneum, and cockroaches originated from different regions of Iran.Methods: The red flour beetles and cockroaches were collected from local bakeries in five cities including Tehran, Ahvaz, Kazerun, and Sabzevar during 2010–2011. Some beetles and cockroaches were colonized in insectary and adults from F1 generation were fed on H. diminuta eggs. Both laboratory-infected and field-collected samples were dissected and examined for cysticercoids. Detection of H. diminuta DNA in T. castaneum beetles was performed by targeting a partial sequence of Ribosomal gene.Results: Except the beetles from Ahvaz, all specimens were negative for cysticercoid by microscopy. Of the four dissected beetles from Ahvaz, one harbored 12 cysticercoids. Also, 110 (52%) of laboratory-infected beetles showed infection with an average of 12–14 larvae. None of the cockroaches was infected. Two beetles from Ahvaz, includ­ing the remainder of the microscopic positive specimen, yielded the expected amplicon in PCR assay. The H. diminuta DNA sequences generated in this study were identical and matched 97–100% with similar sequences from GenBank database.Conclusion: Lack of infection in the majority of beetles may reflect a low rat infestation rate in those areas, alternatively, the examined specimens might not have been the representative samples of the T. castaneum populations.

References

Ballinger-Crabtree ME, Black WC, Miller BR (1992) Use of genetic polymorphisms detected by the random amplified pol- ymorphic DNA polymerase chain reac- tion (RAPD-PCR) for differentiation and identification of Aedes aegypti sub- species and populations. Am J Trop Med Hyg. 47(6): 893–901.

Bisseru B (2013) Diseases of man acquired from his Pets. William Heinemann Med- ical Books Ltd, Elsevier, London.

Bosquet Y (1837) Beetles associated with stored products in Canada: an identifi- cation guide. Agriculture Canada, the University of Michigan.

Cohen I (1989) A case report of a Hymenole- pis diminuta infection in a child in St James Parish, Jamaica. J La State Med Soc. 141(3): 23–24.

d’Ovidio D, Noviello E, Pepe P, Del Prete L, Cringoli G, Rinaldi L (2015) Survey of Hymenolepis spp. in pet rodents in Italy. Parasitol Res. 114(12): 4381–4384.Holt RH (1989) Hymenolepis diminuta uti- lizes the envelope surrounding Monili- formis moniliformis in order to survive in the cockroach host. J Parasitol. pp.160–162.

Kalaivani R, Nandhini L, Seetha KS (2014) Hymenolepis diminuta infection in a school-going child: A rare case report. Australas Med J. 7(9): 379–381.

Karuna T, Khadanga S (2013) A case of Hy- menolepis diminuta in a young male from Odisha. Trop Parasitol. 3(2): 145–147.

Kia E, Homayouni M, Farahnak A, Mohe- bali M, Shojai S (2001) Study of en- doparasites of rodents and their zoono- tic importance in Ahvaz, southwest Iran. Iran J Public Health. 30(1–2): 49–52.

Kılınçel Ö, Öztürk C, Gün E, Öksüz Ş, Uzun H, Şahin İ, Kılıç N (2015) A rare case of Hymenolepis diminuta infection in a small child. Mikrobiyol Bul. 49(1): 135–138.

Macnish M, Ryan U, Behnke J, Thompson R (2003) Detection of the rodent tape- worm Rodentolepis (= Hymenolepis) microstoma in humans. A new zoono- sis? Int J Parasitol. 33(10): 1079–1085.

Magalhaes RJS, Fançony C, Gamboa D, Langa AJ, Sousa-Figueiredo JC, Clements AC, Nery SV (2013) Extending Helminth control beyond STH and Schistosomi- asis: the case of human hymenolepiasis. PLoS Negl Trop Dis. 7(10): e2321.

Makki M, Shahbazi F, Teimoori S, Rokni M, Abaei M, Mobedi I, Hassanpour G, Mowlavi G (2011) Establishment of Hymenolepis diminuta life cycle to provide parasite mass production. Iran J Parasitol. 6 (2): 60–63.

Marangi M, Zechini B, Fileti A, Quaranta G, Aceti A (2003) Hymenolepis diminuta infection in a child living in the urban area of Rome, Italy. J Clin Microbiol.41(8): 3994–3995.

Meshkekar M, Sadraei J, Mahmoodzadeh A, Mobedi I (2014) Helminth infections in Tehran, Iran. Iran J Parasitol. 9(4):548–552.

Mowlavi G, Mobedi I, Mamishi S, Rezaeian M, Ashtiani MH, Kashi M (2008) Hy- menolepis diminuta (Rodolphi, 1819) infection in a child from Iran. Iran J Public Health. 37(2): 120–122.

Nateghpour M, Motevalli-Haghi A, Akbar- zadeh K, Akhavan AA, Mohebali M, Mobedi I, Farivar L (2015) Endopara sites of wild rodents in southeastern Iran. J Arthropod Borne Dis. 9(1): 1–6.

Rau M (1979) The frequency distribution of Hymenolepis diminuta cysticercoids in natural, sympatric populations of Te- nebrio molitor and T. obscurus. Int J Parasitol. 9(2): 85–87.

Sinhabahu V, Perera T, Samarasinghe S (2014) A case of Hymenolepis diminuta (rat tapeworm) infestation in a child. Cey- lon Med J. 59(2): 70–71

Sreedevi C, Kumar PR, Jyothisree C (2015) Hymenolepiasis in a group of albino rats (Rattus albus): a study. J Parasit Dis. 39(2): 321–323.

Tena D, Simón MP, Gimeno C, PomataMTP, Illescas S, Amondarain I, González A, Domínguez J, Bisquert J (1998) Hu- man infection with Hymenolepis diminu- ta: case report from Spain. J Clin Micro- biol. 36(8): 2375–2376.

Tiwari S, Karuna T, Rautaraya B (2014) Hy- menolepis diminuta infection in a child from a rural area: A rare case report. J Lab Physicians. 6(1): 58–59.

Tu W-C, Lai S-C (2006) Induction of cyste ine proteinase in the encapsulation of Hymenolepis diminuta eggs in the Amer- ican cockroach, Periplaneta america- na. J Invertebr Pathol.92(2): 73–78.

Wiwanitkit V (2004) Overview of Hyme- nolepis diminuta infection among Thai patients. MedGenMed. 6(2): 7.

Yan G, Norman S (1995) Infection of Tribo- lium beetles with a tapeworm: varia- tion in susceptibility within and be- tween beetle species and among ge- netic strains. J Parasitol. 81(1): 37–42.

Yousefi A, Eslami A, Mobedi I, Rahbari S, Ronaghi H (2014) Helminth Infections of House Mouse (Mus musculus) and Wood Mouse (Apodemus sylvaticus) from the Suburban Areas of Hamadan City, Western Iran. Iran J Parasitol. 9 (4): 511–518.

Published
2017-06-13
How to Cite
1.
Mowlavi G, Shahbazi F, Abai MR, Najafi F, Hosseini-Farash BR, Teimoori S, Hasanpour H, Naddaf SR. Identification of Hymenolepis diminuta Cysticercoid Larvae in Tribolium castaneum (Coleoptera:Tenebrionidae) Beetles from Iran. J Arthropod Borne Dis. 11(2):338-343.
Section
Short Communication