A Comparative Pathomorphological Findings Between Leiurus abdullahbayrami and Androctonus crassicauda (Scorpion: Buthidae) Envenomation in Rabbit Animal Model

  • Ozcan Ozkan Çankırı Karatekin University, Çankırı, Turkey
  • Mehmet Eray Alcigir Department of Pathology, Kirikkale University, Faculty of Veterinary Medicine, Kirikkale, Turkey
Keywords: Leirus abdullahbayrami, Androctonus crassicauda, venom, pathomorphology, rabbit

Abstract

Background: The aim of study was to compare macroscopical and histopathological findings between venoms be-longing to two scorpion species, Androdoctonus crassicauda, and the newly discovered Leirus abdullahbayrami. Methods: The animals used in this experimental study were fifteen New Zealand bred rabbits. Three groups were constituted as group I (L. abdullahbayrami group, n= 6), group II (A. crassicauda group, n= 6) and group III (control group, n= 3). The animals in the L. abdullahbayrami group and the A. crassicauda group were envenomed through an intravenous route. The rabbits were monitored for the first 24h following the envenomation. The animals dead within that time period were examined and all animals were sacrificed and standard necropsy process was performed at 24h. Results: The pathomorphological findings from group I were found to be more severe than those observed in group II. The venom from the newly identified L. abdullahbayrami has a greater effect than the venom from the A. crassicauda. Moreover, as this was a rabbit modeling study, the L. abdullahbayrami might pose the most serious health threat to infants in particular due to their smaller body weight. Conclusion: These findings will provide a better understanding of envenomation of human beings in terms of the possible consequences of scorpion toxication on the organs.

References

Afifi SH, El-Kashef R, Seddek, AS, Salem, DA (2016) Light and transmission electron microscopical changes associated with Leiurus quınqestrıatus venom in rabbits. Mac Vet Rev. 39(1): 51-57.

Bakir B, Ozkan O, Alcigir ME, Vural SA (2012) Effects of Androctonus crassicauda Scorpion Venom on the Heart Tissue. J Anim Vet Adv. 11(14): 2594-2599.

Barbouche MR, Haguiga H, Nouira S, Krifi MN, Abroug F, Bouchoucha S (1996) Inflammatory cytokines and scorpionic analysis of serological levels in 46 Tunisian patients. Toxicon. 34: 156-157

Bertazzi DT, Assis-Pandochi AI, Azzolini AE, Talhaferro VL, Lazzarini M, Arantes EC (2003) Effect of Tityus serrulatus scorpion venom and its major toxin, TsTX-I, on the complement system in vivo. Toxicon. 41: 501–508.

Bertke EM, Atkins JH (1961) Effect of Centruroides sculpturatus venom up on rat tissue: a histopathologic study. Toxicon. 2: 205-209.

Correa MM, Sampaio SV, Lopes RA, Mancuso LC, Cunha OA, FrancoJJ, Giglio JR (1997) Biochemical and histopathological alterations induced in rats by Tityus serrulatus scorpion venom and its major neurotoxin tityustoxin-1. Toxicon. 35(7): 1053-1067.

Dehghani R, Khamehchian T, Vazirianzadeh B, Vatandoost H, Moravvej SA (2012) Toxic effects of scorpion, Hemiscorpius lepturus (Hemiscorpiidae) venom on mice. J Anim Plant Sci. 22(3): 593-596.

D’Suze G, Salazar V, Diaz P, Sevcik C, Azpurua H, Bracho N (2004) Histopathological changes and inflammatory response induced by Tityus discrepans scorpion venom in rams. Toxicon. 44: 851–860.

Fabiano G, Pezzolla, A, Filograna MA, Ferrarese F (2008) Traumatic shock-physiopathologic aspects. G Chir. 29(1-2): 51–57.

Freire-Maia L, De Matos IM (1993) Heparin or a PAF antagonist (BN-52021) prevents the acute pulmonary edema induced by Tityus serrulatus scorpion in the rat. Toxicon. 31: 1207–1220.

Heidarpour M, Ennaifer E, Ahari H, Srairi-Abid, N; Borchani L, Khalili G, Amini H, Anvar AA, Boubaker S, El-Ayeb M, Shahbazzadeh D (2012) Histopathological changes induced by Hemiscorpius lepturus scorpion venom in mice. Toxicon. 59: 373-378.

Hodhod S, Shoukry S, El-Shimi IR, El-Asmar MF (1997) Effect of scorpion Androctonus amoreuxi (Aud. and Sav) venom on blood and liver of rat. In: Proc. 1st Ain. Shams Medical Cong. 1:535–560.

Ismail M (1995) Review article: the scorpion envenoming syndrome. Toxicon. 33: 825–858.

Krifi, MN, Marrakchi N, El Ayeb M, Dellagi K (1998) Effect of some variables on the in vivo determination of scorpion and viper venom toxicities. Biologicals. 26(4): 277–288.

Magalhaes MM, Pereira ME, Amaral CF, Rezende NA, Campolina D, Bucaretchi F, Gazzinelli RT, Cunha-Melo JR (1999) Serum levels of cytokines in patients envenomed by Tityus serrulatus scorpion sting. Toxicon. 37: 1155–1164.

Mirakabadi AZ, Jalali A, Jahromi AE, Vatanpour H, Akbary A (2006) Biochemical changes and manifestations of envenomation produced by Odonthobuthus doriae venom in rabbits. J Venom Anim Toxins Incl Trop Dis. 12(1): 67–77.

Meki A, Mohey El-Dean ZM (1998) Serum interleukin 1b, interleukin-6, nitric oxide and a1-antitrypsin in scorpion envenomed children. Toxicon. 36:1851–1859.

Novoa E, D’Suze G, Winter M, Crespo A, Tortoledo MA, Marcano H, Friedman E, Sevik C, Zabrer J, Sandchez de Leon R (2003) The indirect effect of Tityus discrepans on rabbit pulmonary vasculature. Respir Physiol Neurobiol. 134(1): 33–41.

Ozkan O, Carhan A (2008) The neutralizing capacity of Androctonus crassicauda antivenom against Mesobuthus eupeus scorpion venom. Toxicon. 52(2): 375–379.

Ozkan O, Yagmur EA, Ark M (2011) A newly described scorpion species, Leiurus abdullahbayrami (Scorpion: Buthidae), and the lethal potency and in vivo effects of its venom. J Venom Anim Toxins incl Trop Dis. 17(4): 414-21.

Ozkan O, Yagmur EA (2017) Neutralization Capacity of Monovalant Antivenom Against Existing Lethal Scorpions in the Turkish Scorpiofauna. Iranian Journal of Pharmaceutical Research. Articles in Press. Available form: http://ijpr.sbmu.ac.ir/?_action=press&issue=-1&_is=Articles%20in%20Press.

Petreicevich VL (2010) Scorpion venom and the inflammatory response. Mediator of Inflamm. 1-10.

Possani LD, Becerril B, Delpierre M, Tytgat J (1999) Scorpion toxins specific for sodium channels. Eur J Biochem. 264: 287–300.

Sofer S, Gueron M, White RM, Lifshitz M, Apte RN (1996) Interleukin-6 release following scorpion sting in children. Toxicon. 34: 389–392,

Zayerzadeh E, Zare MA, Koohi MK (2011) Biochemical and histopathological study of Mesobuthus eupeus scorpion venom in the experimental rabbits. Archiv Razi Inst. 66(2): 133-138.

Published
2019-04-22
How to Cite
1.
Ozkan O, Alcigir M. A Comparative Pathomorphological Findings Between Leiurus abdullahbayrami and Androctonus crassicauda (Scorpion: Buthidae) Envenomation in Rabbit Animal Model. J Arthropod Borne Dis. 13(1):104.
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Original Article