Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom

  • Khalil Pourkhalili Department of Physiology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
  • Hossein Fatemikia Department of Physiology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  • Euikyung Kim College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea
  • Navid Reza Mashayekhy Department of Cardiology, Amir Kabir Hospital, Arak University of Medical Sciences, Arak, Iran
  • Naser Mohammadpour Dounighi Department of Human Vaccine and Serum, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
  • Abdollah Hajivandi Department of Biostatistics, Faculty of Health, Bushehr University of Medical Sciences, Bushehr, Iran
  • Yaghoob Hassan Student Research Committee, Bushehr University of Medical Science, Bushehr, Iran
  • Ramin Seyedian Department of Pharmacology, Bushehr University of Medical Sciences, Bushehr, Iran
Keywords: Hemiscorpius lepturus, Vasoconstriction, Polyvalent antivenom


Background: We investigated the hemodynamic changes (Inotropic, chronotropic and arrhythmogenic) in intrave­nously envenomed anesthetized rats with Hemiscorpius lepturus venom. The neutralizing potencies of different drugs and commercial antivenom were assessed simultaneously.Methods: Different doses of the crude venom (100, 200 and 400μg/rat) were injected during five minutes via the femoral vein and cardiovascular changes were recorded in rats in Razi Institute Corporation, Karaj, Iran in 2017. The drugs (Atropine, lidocaine, propranolol and prazosin) were injected before the venom for determination of the coun­teracting effects. Different volumes (100, 500 and 1000µl) of the antivenom were pre envenomed to neutralize cardi­ovascular changes.Results: Temporary hypertension and bradycardia with no arrhythmogenic effects were depicted within twenty minutes. There was a difference in arterial pressure between the venom (400μg/rat) and the vehicle at 8 minutes (114.68±5.1mmHg versus 70.2±4.3mmHg). Elevation of the mean arterial pressure was inhibited by propranolol (2 mg/kg) and neutralized by prazosin (1mg/kg) while lidocaine (4mg/kg) and atropine (1mg/kg) had no effects. Pre­medication with Iranian commercial antivenom (1000μl) produced surprisingly temporary hypertension compared to the vehicle (140.84±4.5 versus 84.3±3.2). It had no neutralizing properties on blood pressure variation before the venom injection. Volume-expanded hypertension phenomenon was ruled out in a parallel study.Conclusion: This venom has vasoconstrictive effects in rats probably due to the presence of norepinephrine like ma­terials in its content or liberated from adrenal gland inhibited by prazosin premedication. The neutralizing effects of antivenom on venom-induced hypertension are questionable.


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How to Cite
Pourkhalili K, Fatemikia H, Kim E, Mashayekhy NR, Dounighi NM, Hajivandi A, Hassan Y, Seyedian R. Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom. jad. 12(1):31-0.
Original Article