Articles

Adulticidal Activity of Olea vera, Linum usitatissimum and Piper nigera against Anopheles stephensi and Aedes aegypti under Laboratory Conditions

Abstract


Background:
There are several plant extractions which are being used for mosquito control. The aim of this study was to evaluatethe efficacy of Olea vera, Linum usitatissimum and Piper nigera against Anopheles stephensi and Aedes aegypti under laboratory conditions.
Methods: These tests were carried out using WHO recommended bioassay method for adult mosquitoes.
Results: The extracts from black pepper was more effective as adulticide with lowest LC50 values (2.26% and 8.4%) against Aedes aegypti and Anopheles stephensi after 24 h of exposure while after 48h (1.56% and 5.11%) respec­tively. In terms of LC90 value black pepper was best with (8.66% and 30.1%) against Ae. aegypti and An. stephensi after 24 h of exposure while after 48h (4.59% and 17.3%) respectively. In terms of LT50 black pepper took 15 h to kill 50% tested population of Ae. aegypti while against An. stephensi it took more than 2 days. In terms of percentage mortality black pepper kill 84% of the population of Ae. aegypti and 44.75% of the An. stephensi population.
Conclusion: Black pepper showed best results in term of LC50, LC90, LT50 and percentage mortality against Ae. ae­gypti and An. stephensi. Our study suggested that the plant extracts have potential to kill adult mosquitoes, are envi­ronment friendly and can be used for the control of mosquitoes.

Abbot SW (1925) A method of competing the effectiveness of an insecticide. J Econ Entomol. 18: 265–267.

Adebayo TA, Gbolade AA, Olaifa JI (1999) Comparative study of toxicity of es- sential oils to larvae of three mosquito species. Nigerian J Nat Prod Med. 3:74–76.

Akram W, Khan HAA, Hafeez F, Bilal H, Kim YK, Lee JJ (2010) Potential of citrus seed extracts against dengue fe- ver mosquito, Aedes albopictus (Skuse) (Culicidae: Diptera). Pak J Bot. 42(4):3343–3348.

Amer A, Mehlhorn H (2006a) Repellency effect of forty-one essential oils against Aedes, Anopheles, and Culex mosqui- toes. Parasitol Res. 99: 478–490.

Amer A, Mehlhorn H (2006b) The sensilla of Aedes and Anopheles mosquitoes and their importance in repellency. Parasitol Res. 99: 491–499.

Choochote W, Benjawan T, Duangta K,Eumporn R, Udom C, Prasong C, Atchariya J, Pongsri T, Doungrat, Ben- jawan (2004) Potential Of crude seed extract of celery, Apium graveolens L., against the Mosquito Aedes aegypti (L.) (Diptera: Culicidae). J Vector Ecol.29: 340–346.

Curtis CF, Jana-Kara B, Maxwell CA (2003) Insecticide treated nets: impact on vector populations and relevance of initial intensity of transmission and py- rethroid resistance. J Vect Borne Dis.40: 1–9.

Dengue and dengue haemorrhagic fever.Geneva, World Health Organization, March (2009), Fact sheet N°117.

Dua VK, Pandey AC, Dash AP (2010) Adul- ticidal activity of essential oil of Lan- tana camara leaves against mosqui- toes. Indian J Med Res. 131: 434–439.

Finney DJ (1989) Probit analysis, 3rd ed.Cambridge Great Britain University press, UK.

Flood response in Pakistan. Weekly Epide- miological Bulletin (2010). 1(10): 6.

Gbolade AA, Oyedele AO, Sosan MB, Adewoyin FB, Soyelu O (2000) Mos- quito repellent activities of essential oils from two Nigerian Ocimum spe- cies. J Trop Med Plants.1: 146–148.

Guidelines for testing mosquito adulticides for Indoor residual spraying and treat- ment of mosquito nets. Geneva, World Health Organization, (2006) (WHO/ CDS/NTD/WHOPES/GCDPP).

Hafeez F, Waseem A, Essam S (2011) Mos- quito larvicidal activity of citrus limon- oids against Aedes albopictus. Parasit Res. 109(1): 221–229.

Isman MB (2000) Plant essential oils for pest and disease management. Crop Prot. 19: 603–608.

Jaswanth A, Ramanathan P, Ruckmani K (2002) Evaluation of mosquitocidal activity of Annona squamosa leaves against filarial vector mosquito, Culex quinquefasciatus Say. Indian J Experi- mental Biol. 40(3): 363–365.

Joseph CC, Malima RC, Mohd-Hussein ZA, Jantan I, Awaludin A, Wasi AN (2001) Insecticidal activity of the methanol extracts of Some tunicate species against Aedes aegypti and Anopheles macu- lates. Pharm Biol. 39(3): 213–216

Murgan K, Murgan P, Noortheen A (2007) Larvicidal and repellent potential of Albizzia amara Boivin and Ocimum basilium Linn against dengue vector, Aedes aegypti. Biores Technol. 98:198–201.

Ndoile MM, Nkunya MHH (2004) Lar- vicidal and mosquitocidal extracts, a coumarin, isoflavonoids and pterocar- pans from Neorautanenia mitis. Transac. Royal Soc Trop Med Hygiene. 98(8):451–455

Raghavendra K, Subbarao SK (2002) Che- mical insecticides in malaria vector control in India. ICMR Bulletin. 32(10):1–7.

Rozendaal JA (1997) Vector Control-Meth- ods for Use by Individuals and Com- munities. Geneva, World Health Or- ganization.

Shaalan EAS, Canyon DV, Bowden B,Younes MWF, Wahab HA, Mansoin AH (2006) Efficacy of botanical ex- tracts from Callitris glaucophylla against Aedes aegypti and Culex annulirostris mosquitoes. Trop Biomed, 2: 180–185.

Sulaiman S, Abang Kamarudin DSF, Oth- man H (2008) Evaluation of Bifenthrin and Acorus calamus Linn. Extract against Aedes aegypti L. and Aedes al- bopictus (Skuse). Iran J Arthropod- Borne Dis. 2(2): 7–11.

Tripathi AK, Prajapati V, Verma N, Bahl JR, Bansal RP, Khanuja SP, Kumar S (2002) Bioactivities of the leaf essen- tial oil of Curcuma Longa (var. ch-66) on three species of stored-product beetles (Coleoptera). J Econ Entomol.95: 183–189.

Tripathi AK, Veena P, Ateeque A, Kishan KA, Suman PSK (2004) Piperitenone oxide as toxic, repellent and reproduc- tion retardant toward malarial vector Anopheles stephensi (Diptera: Anopheli- nae). J Med Entomol. 41(4): 691–698.

Trongtokit Y, Burivong P, Moore S, Hill N, Curtis CF (2008) Plant extracts in pre- vention of mosquito attacks. Botanical medicine in clinical practice. pp. 807–813. ISBN (978-1-84593-413-2)

Tsao R, Lee S, Rice PJ, Jensen C, Coats JR (1995) Monoterpenoids and their syn- thetic derivatives as leads for new in- sect control agents. In: Baker, DR, Fenyes, JG, Basarab, GS (Eds), Syn- thesis and Chemistry of Agrochemi- cals IV. American Chemical Society, Washington, DC. pp. 312–324.

Vogel AI (1978) Text Book of Practical Or- ganic Chemistry. The English language Book Society and Longman, London.Weekly morbidity and mortality report. Epi- demiological Week No. 46 (11–17 Nov2006). 23 Nov 06/ Vol. 52/ DEWS 2006-46.

WHO (World Health Organization) (1975) Manual on practical Entomology in Ma- laria, World Health Organization. Pre- pared by the WHO Division of Malaria and other parasites diseases. Part 1–2. Offset publication No.13. World Health Organization, Geneva.

World Malaria Report Pakistan. Geneva, World Health Organization (2009). Pp 135.

World Malaria Report. Geneva, World Health Organization (2009). pp 37.

Zaim M, Jambulingam P (2004) Global in- secticide use for vector borne disease control. World Health Organization Communicable Disease Control, Pre- vention and Eradication, WHO Pesti- cide Evaluation Scheme (WHOPES).

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Keywords
Adulticide Plant extracts Anopheles stephensi

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1.
Nawaz R, Rashid Rathor H, Bilal H, Hassan S, Akram Khan I. Adulticidal Activity of Olea vera, Linum usitatissimum and Piper nigera against Anopheles stephensi and Aedes aegypti under Laboratory Conditions. J Arthropod Borne Dis. 1;5(2):2-9.