Essential Oil Composition and Larvicidal Evaluation of Platycladus orientalis against Two Mosquito Vectors, Anopheles stephensi and Culex pipiens
Abstract
Background: Natural plant products as larvicides could be considered as desirable alternatives to synthetic chemical insecticides for vector management. This study was undertaken to assess the mosquito larvicide activity of the essential oil from fresh leaves of Platycladus orientalis against two medically important species of mosquito vectors.
Methods: Essential oil was extracted by hydrodistillation and analyzed with gas chromatography and mass spectrometry (GC-MS). Fresh leaves of P. orientalis tree (500g) were collected in June 2014 from Tehran, Iran and was authenticated at the Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
In addition, the larvicidal potential of oil was evaluated against late-3rd or young-4th instar larvae of Anopheles stephensi and Culex pipiens under laboratory condition. The mortality counts were made after 24h and LC50 and LC90 values were calculated.
Results: Forty-six components in leaves of P. orientalis were identified. The major components were α-Pinene (20.17%), 3-Carene (14%) and Cedrol (9.51%). The LC50 values against An. stephensi and Cx. pipiens larvae were 11.67ppm and 18.60ppm after 24h, respectively.
Conclusion: Platycladus orientalis oil could be considered as a natural larvicide for mosquito larval control.
Service M (2012) Medical Entomology For Students. Cambridge University Press, Cambridge.
WHO (2016) World malaria report 2016. WHO Press, Geneva, Switzerland.
Becker N, Petrić D, Boase C, Lane J, Zgomba M, Dahl C, Kaiser A (2010) Mosquitoes And Their Control. Springer, Berlin.
Sedaghat MM, Linton YM, Nicolescu G, Smith L, Koliopoulos G, Zounos AK, Oshaghi MA, Vatandoost H, Harbach RE (2003) Morphological and molecular characterization of Anopheles (Anopheles) sacharovi Favre, a primary vector of malaria in the Middle East. Syst Entomol. 28: 241–256.
Sedaghat MM, Linton YM, Oshaghi MA, Vatandoost H, Harbach RE (2003) The Anopheles maculipennis complex (Diptera: Culicidae) in Iran: molecular characterization and recognition of a new species. Bull Entomol Res. 93: 527–535.
Sedaghat MM, Harbach RE (2005) An annotated checklist of the Anopheles mosquitoes (Diptera: Culicidae) in Iran. J Vector Ecol. 30: 272–276.
Shayeghi M, Vatandoost H, Gorouhi A, Sanei-Dehkordi AR, Salim-Abadi Y, Karami M, Jalil-Navaz MR, Akhavan AA, Shiekh Z, Vatandoost S (2014) Biodiversity of aquatic insects of Zayandeh Roud River and its branches, Isfahan Province, Iran. J Arthropod Borne Dis. 8(2): 197–203.
Georghiou GP (2012) Pest Resistance to Pesticides. Springer Science and Business Media, Berlin.
Edwards C (2013) Environmental Pollution by Pesticides.Vol. 3. Springer Science and Business Media, Berlin.
Isman MB (2000) Plant essential oils for pest and disease management. Crop Prot. 19: 603–608.
Isman MB, Machial CM, Miresmailli S, Bainard LD (2007) Essential Oil-Based Pesticides: New Insights From Old Chemistry. Wiley-VCH, Weinheim.
Soleimani-Ahmadi M, Abtahi SM, Madani A, Paksa A, Salim Abadi Y, Gorouhi MA, Sanei-Dehkordi A (2013) Phytochemical profile and mosquito larvicidal activity of the Essential oil from aerial parts of Satureja bachtiarica Bunge against malaria and lymphatic filariasis vectors. Acta Med Iran. 51: 141–147.
Sanei-Dehkordi A, Soleimani-Ahmadi M, Akbarzadeh K, Salim Abadi Y, Paksa A, Gorouhi MA, Mohammadi-Azni S (2016a) Chemical composition and mosquito larvicidal properties of essential oil from leaves of an Iranian indigenous plant Zhumeria majdae. J Essent Oil Bear Pl. 19: 1454–1461.
Soleimani-Ahmad M, Gorouhi MA, Abadi YS, Paksa A, Rashid G, Sanei-Dehkordi A (2017) Larvicidial Effects of essential oil and methanol extract of Achillea wilhelmsii C. Koch (Asteraceae) against Anopheles stephensi Liston (Diptera: Culicidae), a malaria vector. J Kerman Univ Med Sci. 24: 58–67.
Asadi M (2001) Flora of Iran. Families of Pinaceae, Taxaceae, Cupressaceae and Ephedraceae. Research Institute of Forests and Rangelands, Tehran (In Persian).
Mir-Heydar H (2006) Maaref Ghiahi (Plant Knowledge). Vol. 2. Daftar-e Nashr-e Eslami, Tehran.
Hassanzadeh M, Rahimizadeh M, Bazzaz BF, Emami S, Assili J (2001) Chemical and antimicrobial studies of Platycladus orientalis essential oils. Pharm Biol. 39: 388–390.
Guleria S, Kumar A (2006) Antifungal activity of some Himalayan medicinal plants using direct bioautography. J Cell Mol Bio. 5: 95–98.
Li YY, Hao Sh, Wan DW, Li JY, Sun WG
Meng ZL (2008) Antifungal activity of ethanol extract from Platycladus orientalis. Acta Bot Boreal Occident Sin. 5: 1056–1060.
Jafarian-Dehkordi A, Emami S, Saeidi M, Sadeghi H (2004) Cytotoxicologic studies of the extracts of iranian Juniperus sabina and Platycladus orientalis on cancer cells. J Res Med Sci. 9: 205–209.
Singh A, Singh V (2009) Molluscicidal ac-tivity of Saraca asoca and Thuja orientalis against the fresh water snail Lymnaea acuminata. Vet Parasitol. 164: 206–210.
Hashemi SM, Safavi SA (2012) Chemical constituents and toxicity of essential oils of oriental arborvitae, Platycladus orientalis (L.) Franco, against three stored-product beetles. Chil J Agr Res. 72: 188–194.
Ju-Hyun J, Sang-Hyun L, Moo-Key K, Hoi-Seon L (2005) Larvicidal activity of Chamaecyparis obusta and Thuja orientalis leaf oils against two mosquito species. Agric Chemiand Biotech. 48 (1): 26–28.
Adams RP (2007) Identification of es-sential oil components by Gas Chromatography/Mass Spectroscopy. Allured Publishing Co. Carol Stream, Illinois.
Afsharypuor S, Nayebzadeh B (2009) Es¬sential oil constituents of young stem, leaf and fruit of Platycladus orientalis (L.) Franco grown in Isfahan (Iran). J Essent Oil Res. 21: 525–528.
Lei H, Wang Y, Liang F, Su W, Feng Y, Guo X, Wang N (2010) Composition and variability of essential oils of Platycladus orientalis growing in China. Biochem Syst Ecol. 38: 1000–1006.
WHO (1981) Instructions for Determining the Susceptibility or Resistance of Mosquito Larvae to Insecticides. World Health Organization, Geneva.
Abbott W (1925) A method of computing the effectiveness of an insecticide. J Econ
Entomol.18: 265–267.
Finney DJ (1971) Probit Analysis. 3rd Ed. Cambridge University Press, Cambridge.
Guleria S, Kumar A, Tiku AK (2008) Chemical composition and fungitoxic activity of essential oil of Thuja orientalis L. grown in the north-western Himalaya. Z Naturforsch C. 63: 211–214.
Nickavar B, Amin G, Parhami S (2003) Volatile constituents of the fruit and leaf oils of Thuja orientalis L. grown in Iran. Z Naturforsch C. 58: 171–172.
Sedaghat MM, Dehkordi AS, Abai MR, Khanavi M, Mohtarami F, Salim Abadi Y, Rafi F, Vatandoost H (2011a) Larvicidal activity of essential oils of Apiaceae plants against malaria vector, Anopheles stephensi. J Vector Borne Dis. 5: 51–59.
Sedaghat MM, Dehkordi AS, Khanavi M, Abai MR, Mohtarami F, Vatandoost H (2011b) Chemical composition and larvicidal activity of essential oil of Cupressus arizonica EL Greene against malaria vector Anopheles stephensi Liston (Diptera: Culicidae). Pharm Res. 3: 135–139.
Sedaghat MM, Sanei AR, Khanavi M, Abaei MR, Abbas H, Mohtarami F, Vatandoost H (2010) Phytochemistry and larvicidal activity of Eucalyptus camaldulensis against malaria vector, Anopheles stephensi. Asian Pac J Trop Med. 3: 841–845.
Mozaffari E, Abai MR, Khanavi M, Vatandoost H, Sedaghat MM, Moridnia A, Saber-Navaei M, Sanei-Dehkordi A, Rafi F (2014) Chemical composition, larvicidal and repellency properties of Cionura erecta (L.) Griseb. against malaria vector, Anopheles stephensi Liston (Diptera: Culicidae). Iran J Arthropod Borne Dis. 8: 147–155.
Sanei-Dehkordi A, Sedaghat MM, Vatandoost H, Abai MR (2016b) Chemical
compositions of the Peel essential oil of Citrus aurantium and its natural larvicidal activity against the malaria vector Anopheles stephensi (Diptera: Culicidae) in comparison with Citrus paradisi. J Vector Borne Dis. 10: 577–585.
Sanei-Dehkordi A, Vatandoost H, Abaei MR, Davari B, Sedaghat MM (2016c) Chemical composition and larvicidal activity of Bunium persicum essential oil against two important mosquitoes vectors. J Essent Oil Bear Pl. 19: 349–357.
Vatandoost H, Dehkordi AS, Sadeghi S, Davari B, Karimian F, Abai M, Sedaghat M (2012) Identification of chemi¬cal constituents and larvicidal activity of Kelussia odoratissima Mozaffarian essential oil against two mosquito vec¬tors Anopheles stephensi and Culex pipiens (Diptera: Culicidae). Exp Par¬asitol. 132: 470–474.
Cheng SS, Chang HT, Chang ST, Tsai KH, Chen WJ (2003) Bioactivity of selected plant essential oils against the yellow fever mosquito Aedes aegypti larvae. Bioresource Technol. 89: 99–102.
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Issue | Vol 12 No 2 (2018) | |
Section | Original Article | |
DOI | https://doi.org/10.18502/jad.v12i2.35 | |
Keywords | ||
Anopheles stephensi Culex pipiens Platycladus orientalis Essential oil Larvicide |
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