Journal of Arthropod-Borne Diseases 2011. 5(2):60-68.

Neospora caninum and Leishmania infantum Co-Infection in Domestic Dogs (Canis familiaris) in Meshkin-Shahr District, Northwestern Iran
M Sharifdini, M Mohebali, H Keshavarz, M Hosseininejad, H Hajjaran, B Akhoundi, A Rahimi Foroushani, Z Zarei, S Charehdar

Abstract


Background: Mediterranean visceral leishmaniasis (MVL)is an infectious disease that affects both human and ani­mals. Domestic dogs (Canis familiaris) are principal reservoir hosts of MVL caused by Leishmania infantum. Dogs are definitive hosts for Neospora caninumand a risk factor for infecting intermediate hosts. The immunosuppression caused by visceral leishmaniasis (VL) can promote the occurrence of co-infections with other agents such as neosporo­sis. This study aimed to determine the frequency of co-infection of the both protozoan parasites in the en­demic areas of VL from Meshkin-Shahr District, north-west of Iran.
Methods: Altogether, 171 serum samples were collected from domestic dogs of Meshkin- Shahr District by multistage cluster sampling from October 2008 to August 2009. The collected serum samples were tested for the detection of simultaneous infection of L. infantum and N. caninum using direct agglutination test (DAT) and indirect ELISA, respectively.
Results: Of the 171 domestic dogs, 27 (15.8%) and 52 (30.4%) were showed antibodies against L. infantum and N. caninum, respectively. Simultaneous infections of N. caninum and L. infantum was found in 16 (9.4%) of the dogs. In VL-positive and VL-negative dogs, N. caninum infection was found in 59.3% and 25.0%, respectively. A statisti­cally significant difference was found between VL-positive and VL-negative dogs with N. caninum infection (P= 0.001).
Conclusion: These findings indicate that Meshkin-Shahr District in northwestern Iran is an active focus of canine visceral leishmaniasis (CVL). Neospora caninum and L. infantum co-infection is prevalent in the area and infection by L. infantum seems to enhance susceptibility to N. caninum infection in domestic dogs.


Keywords


Neospora caninum, Leishmania infantum, Co-infection, Seroepidemiology, dog, Iran,

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References


Alvar J, Canavate C, Molina R, Moreno J, Nieto J (2004) Canine leishmaniasis. Adv Parasitol. 57: 1–88.

Andreotti R, Oliveira JM, Silva EA, Oshiro LM, Matos Mde F (2006) Occurrence of Neospora caninum in dogs and its correlation with visceral leishmaniasis in the urban area of Campo Grande, Mato Grosso do Sul, Brazil. Vet Parasitol. 135:375-379.

Arias JR, Monteiro PS, Zicker F (1996) The re-emergence of visceral leishmaniasis in Brazil. Emerg Infect Diseases. 2: 145-146.

Bokaei S, Mobedi I, Edrissian GhH, Nadim A (1998) Seroepidemiological study of canine visceral leishmaniasis in Meshkin Shahr, northwest of Iran. Arch Inst Razi. 48–49: 41–46.

Cardoso L, Rodrigues M, Santos H, Schoone GJ, Carreta P, Varejao E, van Benthem B, Afonso MO, Alves-Pires C, Semiao- Santos SJ, Rodrigues J, Schallig HD (2004) Sero-epidemiological study of canine Leishmania spp. infection in the municipality of Alijo (Alto Douro, Portu- gal). Vet Parasitol. 121: 21–32.

Cringoli G, Capuano F, Veneziano V, Romano L, Solimene R, Barber JS, Trees A J (1996) Prevalence of antibodies against Neospora caninum in dog sera. Paras- sitologia. 38: 283.

Cringoli G, Rinaldi L, Capuano F, Baldi L, Veneziano V, Capelli G (2002) Se- rological survey of Neospora caninum and Leishmania infantum co-infection in dogs. Vet Parasitol. 106: 307–313.

Dijkstra T, Barkema HW, Eysker M, Hesselink JW, Wouda W (2002) Natural transmission routes of Neospora caninum between farm dogs and cattle. Vet Para- sitol. 105: 99–104.

Dubey JP (2003) Review of Neospora can- inum and neosporosis in animals. Korean J Parasitol. 41: 1–16.

Dubey JP, Lindsay DS (1989) Transplacen-tal Neospora caninum infection in dogs. Am J Vet Res. 50: 578–1579.

Dubey JP, Lindsay DS (1996) A review of Neospora caninum and neosporosis. Vet Parasitol. 67: 1–59.

Edrissian GhH, Hajjaran H, Mohebali M, Soleimanzadeh G, Bokaei S (1996) Application and evaluation of direct ag- glutination test in ser-diagnosis of vis- ceral leishmaniasis in man and canine reservoirs in Iran. Iranian J Med Sci.21: 119–124.

Fernandes BC, Gennari SM, Souza SL, Carvalho JM, Oliveira WG, Cury MC (2004) Prevalence of anti-Neospora can- inum antibodies in dogs from urban, periurban and rural areas of the city of Uberlandia, Minas Gerais-Brazil. Vet Parasitol. 123: 33–40.

Gavgani AS, Mohite H, Edrissian GH, Mohebali M, Davies CR (2002) Do- mestic dog ownership in Iran is a risk factor for human infection with Leishma- nia infantum. Am J Trop Med Hyg.67: 511–515.

Haddadzadeh HR, Sadrebazzaz A, Malmasi A, Talei Ardakani H, Khazraii Nia P, Sadreshirazi N (2007) Seroprevalence of Neospora caninum infection in dogs from rural and urban environments in Te- hran, Iran. Parasitol Res. 101: 1563-1565.

Harith A, Reiter I, Knapen F, Korte P, Huigen E, Kolk RHG (1989) Application of a direct agglutination test for detec- tion of specific anti-leishmania antibod- ies in the canine reservoir. J Clin Mi- crobiol. 27: 2252–2257.

Hosseininejad M, Hosseini F, Mosharraf M, Shahbaz S, Mahzounieh M, Schares G (2010a) Development of an indirect ELISA test using an affinity purified sur- face antigen (P38) for sero-diagnosis of canine Neospora caninum infection. Vet Parasitol. 171(3–4): 337–342.

Hosseininejad M, Hosseini F, Mahzounieh M, Raisi Nafchi A, Mosharraf M (2010b) Seroprevalence of Neospora caninum infection in dogs in Chaharma- hal-va-Bakhtiari Province, Iran. Comp Clin Pathol. 19: 269–270.

King JS, Slapeta J, Jenkins DJ, Al-Qassab SE, Ellis JT, Windsor PA (2010) Aus- tralian dingoes are definitive hosts of Neospora caninum. Int J Parasitol.40(8): 945–950.

Malmasi A, Hosseininejad M, Haddadzadeh H, Badii A, Bahonar A (2007) Serologic study of anti-Neospora caninum anti- bodies in household dogs and dogs living in dairy and beef cattle farms in Tehran, Iran. Parasitol Res. 100: 1143–1145.

Mohebali M, Edrissian GhH, Nadim A, Hajjaran H, Akhoundi B, Hooshmand B, Zarei Z, Arshi S, Mirsamadi N, Manouchehri-Naeini K, Mamishi S, Sanati AA, Moshfeh AA, Charehdar S, Fakhar M (2006) Application of Direct agglutination test (DAT) for the diagnosis and seroepidemiological stud- ies of visceral leishmaniasis in Iran. Ira- nian J Parasitol. 1: 15–25.

Mohebali M, Hajjaran H, Hamzavi Y, Mobedi I, Arshi S, Zarei Z, Akhoundi B, Naeini KM, Avizeh R, Fakhar M (2005) Epidemiological aspects of canine visceral leishmaniosis in the Islamic Re- public of Iran. Vet Parasitol. 129: 243–251.

Mohebali M, Taran M, Zarei Z (2004) Rapid detection of Leishmania infantum infec- tion in dogs: comparative study using an immunochromatographic dipstick rk39 test and direct agglutination. Vet Para- sitol. 121: 239–245.

Moshfe A, Mohebali M, Edrissian G, Zarei Z, Akhoundi B, Kazemi B, Jamshidi S, Mahmoodi M (2009) Canine visceral leishmaniasis: asymptomatic infected dogs as a source of L. infantum infec- tion. Acta Trop. 112: 101–105.

Moshfe A, Edrissian GhH, Zarei Z, Ak- houndi B, Kazemi B, Jamshidi Sh, Mahmoodi M (2008) Seroepidemi- ological Study on Canine Visceral Leishmaniasis in Meshkin-Shahr District, Ardabil Province, Northwest of Iran during 2006–2007. Iranian J Parasitol.3: 1–10.

Tarantino C, Rossi G, Kramer LH, Perrucci S, Cringoli G, Macchioni G (2001) Leishmania infantum and Neospora caninum simultaneous skin infection in a young dog in Italy. Vet Parasitol.102: 77–83.

World Health Organization (WHO), (1990).

Control of the leishmaniases WHO Technical Report Series 793: 27.

Yakhchali M, Javadi S, Morshedi A (2010) Prevalence of antibodies to Neospora caninum in stray dogs of Urmia, Iran. Parasitol Res. 106(6):1455–1458


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