Morphometric Indices and Venom Protein Profile in Different Populations of Androctonus crassicauda

Mohammad Bagher Ghavami; Zohreh Alibabaei; Fatemeh Ghavami

doi:10.18502/jad.v16i1.11188

Morphometric Indices and Venom Protein Profile in Different Populations of Androctonus crassicauda

Mohammad Bagher Ghavami

Zohreh Alibabaei

Fatemeh Ghavami

Abstract

Background: Androctonus crassicauda is the most medically relevant animal and understanding its morphological characteristics is essential in the production of antiscorpion sera.

Methods: Adults of A. crassicauda were collected from different areas of Zanjan Province and the morphometric parameters and the cuticular fluorescence patterns of samples were studied. The crude venom of samples was extracted by electric stimulation, and their biochemical properties were analyzed by the SDS-PAGE method.

Results: Values of the morphometric parameters depended on sex and altitude of the area. Except for values of the pectinal organ, these parameters in females were higher than in males. No significant difference was in the number, shape, and intensity of cuticular fluorescence patterns. The body length of males in high and lowlands was 72.53±1.53 and 77.33±2.70mm, respectively. Females' body lengths in that area were 81.66±2.19 and 86.55±2.33mm, respectively. Analysis of toxin proteins showed two isotypes that the 12, 13, 15, 16, 18, and 19kDa proteins were in all areas. However, the 41 and 74kDa proteins, and 46 and 63kDa proteins were detected in low and highlands, respectively.

Conclusion: Black fat-tailed scorpion has a considerable dominancy and developing preventive programs and providing treatment facilities in studied areas are necessary. Values of the morphological parameters and venom electrophoresis patterns depended on the geographical location. Therefore, pool crude toxin is suggested for the production of effective antivenoms. Moreover, additional field complementary works in the geographic information system based niche modeling and mass fingerprinting of scorpion venoms are suggested for screening effective isotypes.

1. Mullen GR, Stockwell SA (2019) Scorpions. In: Mullen GR, Durden LA (Eds): Medical and Veterinary Entomology, 3rd Edition. Elsevier, London, pp. 489–501.
2. Radmanesh M (1990) Androctonus crassi-cauda sting and its clinical study in Iran. Am J Trop M Hyg. 93(5): 323–326.
3. Ismail M, Abd-Elsalam MA, Al-Ahaidib MS (1994) Androctonus crassicauda (Olivier), a dangerous and unduly neglected scorpion-I. Pharmacological and clinical studies. Toxicon. 32(12): 1599–1618.
4. Rodriguez de la Vega RC, Possani LD (2005) Overview of scorpion toxins specific for Na+ channels and related peptides: biodiversity, structure-function relationships and evolution. Toxicon. 46(8): 831–844.
5. Dehghani R, Fathi B (2012) Scorpion sting in Iran: a review. Toxicon. 60(5): 919–933.
6. Stockmann R (2015) Introduction to Scorpion Biology and Ecology. In: Gopala-krishnakone P, Possani LD, Schwartz EF, Rodríguez de la Vega RC (Eds): Scorpion Venoms. Springer, New York, pp. 25–59.
7. Ozkan O, Adiguzel S, Kar S (2006) Parametric values of Androctonus crassicau-da (Oliver, 1807) (Scorpions: Buthidae) from Turkey. J Venom Anim Toxins incl Trop Dis. 12(4): 549–559.
8. Ebrahimi M, Azizi K, Moemenbellah-Fard MD, Fakoorziba MR, Soltani A (2015) Morphometry Indices of the Black Fat-tailed Scorpion Androctonus crassicauda (Scorpiones Buthidae), from Fars Province, Southern Iran. J Entomol. 12(1): 39–47.
9. Volschenk ES (2005) A new technique for examining surface morpho-sculpture of scorpions. J Archnol. 33(3): 820–825.
10. Ramires EN, Peracetta LF, Nogas C, Na-varro-Silva MA, Paladini EP (2013) Equipment based on high-power UV and white light LEDs to collect and observe scorpions (Arachnida: Scorpiones) and other fluorescent organisms. Zoologia (Curitiba). 30(4): 463–466.
11. Kloock CT, Kubli A, Reynolds R (2010) Ultraviolet light detection: a function of scorpion fluorescence. J Archnol. 38(3): 441–445.
12. Gaffin DD, Bumm LA, Taylor MS, Popokina NV, Mann S (2012) Scorpion fluorescence and reaction to light. Anim Behav. 83(2): 429–436.
13. Kloock CT (2008) A comparison of fluorescence in two sympatric scorpion species. J Photochem Photobiol B: Biol. 91 (2–3): 132–136.
14. Caliskan F, García BI, Coronas FI, Batista CV, Zamudio FZ, Possani LD (2006) Characterization of venom components from the scorpion Androctonus crassi-cauda of Turkey: peptides and genes. Toxicon. 48(1): 12–22.
15. Caliskan F, García BI, Coronas FI, Resta-no-Cassulini R, Korkmaz F, Sahin Y, Corzo G, Possani LD (2012) Purification and cDNA cloning of a novel neurotoxic peptide (Acra3) from the scorpion Androctonus crassicauda. Peptides. 37(1): 106–112.
16. Caliskan F, Quintero-Hernández V, Resta-no-Cassulini R, Coronas-Valderrama FI, Corzo G, Possani LD (2013) Molecular cloning and biochemical characteriza¬tion of the first Na+-channel α-type toxin peptide (Acra4) from Androctonus cras-sicauda scorpion venom. Biochimie. 95 (6): 1216–1222.
17. Quintero-Hernández V, Jiménez-Vargas JM, Gurrola GB, Valdivia HH, Possani LD (2013) Scorpion venom components that affect ion-channels function. Toxicon. 76: 328–342.
18. Jalali A, Rahim F (2014) Epidemiological
review of scorpion envenomation in Iran. Iran J Pharm Res. 13(3): 743.
19. Moradi M, Yagmur E, Pooyan-Moradi G, Ahmadi F (2015) Scorpion Fauna of Zanjan Province, Iran (Arachnida: Scor-piones). J Appl Biol Sci. 9(1): 11–14.
20. Farzanpey R (1987) Scorpion Knowledge. Iran Academic Press, Tehran.
21. Mirshamsi O, Sari A, Elahi E, Hosseinie S (2011) Mesobuthus eupeus (Scorpiones: Buthidae) from Iran: A polytypic species complex. Zootaxa. 2929: 1–21.
22. Ozkan O, Ciftci G (2010) Individual variation in the protein profile of the venom of Mesobuthus gibbosus (Brullé, 1832, Scorpiones: Buthidae) from Turkey. J Venom Anim Toxins Incl Trop Dis. 16 (3): 505–508.
23. Ozkan O, Yağmur EA (2017) Neutralization capacity of monovalent antivenom against existing lethal scorpions in the Turkish scorpio fauna. Iran J Pharm Res. 16(2): 653.
24. Saganuwan SA (2018) Determination of median effective dose (ED50) of scorpion antivenom against scorpion enven-omation using a newly developed formula. Animal Model Exp Med. 1(3): 228–234.
25. El-Hafny B, Chgoury F, Adil N, Chen N, Nassar M (2002) Intraspecific variability and pharmacokinetics, characteristics of Androctonus mauretanicus scorpion venom. Toxicon. 40(11): 1609–1616.
26. El Ayeb M, Rochat H (1985) Polymor-phism and quantitative variations of toxins in the venom of the scorpion Androctonus australis hector. Toxicon. 23 (5): 755–760.
27. Badhe RV, Thimas AB, Harer SL, Desphande AD, Salvi N, Waghmare A (2006) Intraspecific variation in protein of red scorpion (Mesobuthus tamulus, Coconsis, Pocock) venoms from Western and Southern India. J Venom Anim Toxins Incl Trop Dis. 12(4): 612–619.
28. Omran MA, Mcvean A (2000) Intraspecific variation in scorpion Leiurus quin-questriatus venom collected from Egypt (Si¬nai and Aswan deserts). J Toxicol Toxin Rev. 19(3–4): 247–264.
29. Abdel-Rahman MA, Omran MAA, Ab-del-Nabi IM, Veda V, McVean A (2009) Intraspecific variation in the Egyptian scorpion Scorpio maurus palmatus venom collected from different biotopes. Toxicon. 53: 349–359.

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Issue	Vol 16 No 1 (2022)
Section	Original Article
DOI	https://doi.org/10.18502/jad.v16i1.11188
Keywords
Androctonus crassicauda Black fat-tailed scorpion ; Morphometric parameters Scorpion ven¬om SDS-PAGE Scorpion venom extraction

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Ghavami M, Alibabaei Z, Ghavami F. Morphometric Indices and Venom Protein Profile in Different Populations of Androctonus crassicauda. J Arthropod Borne Dis. 2022;16(1):13–25.

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