Seroprevalence and Risk Factors of Ehrlichia canis Infection among Companion Dogs of Mashhad, North East of Iran, 2009–2010

  • Maneli Ansari-Mood Mail Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Javad Khoshnegah Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
  • Mehrdad Mohri Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
  • SeyedMehdi Rajaei Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Keywords:
Ehrlichia canis, Indirect Immunofluorescence Assay, Prevalence, Dog, IFA

Abstract

Background: The aims of this study were to determine the seroprevalence of canine ehrlichiosis and risk factors of this disease in companion dogs’ population of Mashhad, North East of Iran. Canine Monocytic Ehrlichiosis (CME) is a zoonotic disease transmitted by ticks, Rhipicephalus sanguineus, and caused by an obligate intracellular bacterium, Ehrlichia canis.Methods: During September 2009 until November 2010, 250 companion dogs from Mashhad, North-East of Iran, were examined for serum antibody detection against E. canis by means of immunofluorescence assay test (IFAT) and factors associated with a positive antibody response.Results: There was a very low prevalence of anti-E. canis antibodies (0.8%, 2/250) among studied dogs. The antibody titers for two seropositive dogs were 1:80 and 1:160, respectively. One (0.4%) of seropositive dogs was infested with, R. sanguineus. In blood smears from one of infested dogs (0.4%), typical morulae of E. canis was observed in lymphocytes. The results confirm that the lowest occurrence of reactive dogs indoors probably related to low tick infestion.Conclusion: This is the first report that describes serological evidences of canine monocytic ehrlichiosis in North- East of Iran. Results suggested that E. canis infection in owned pet dogs from North of Khorasan was not endemic from 2009 to 2010. Additional molecular studies are necessary to confirm E. canis infection and to identify the local strains of the organism.

References

Akhtardanesh B, Ghanbarpour R, Blourizadeh (2010) Serological evidence of canine monocytic ehrlichiosis in Iran. J Comp Clin Path. 19: 469–474.

Amusategui I, Tesouro MA, Kakoma I, Sainz A (2009) Serological reactivity to Ehrlichia canis, Anaplasma phagocytophilum, Neorickettsia risticii, Borrelia burgdorferi and Rickettsia conorii in dogs from northwestern Spain. Vector Borne Zoonotic Dis. 8:797–803.

Anderson BE, Greene CE, Jones DC, Daw- son JE (1992) Ehrlichia ewingii sp. nov, the etiologic agent of canine granulocytic ehrlichiosis. Int J Syst Bacteriol. 42: 299–302.

Anonymous (2012) Companion Animal Parasite Council, Ticks. Available at: http://www.capcvet.org/capc-recom- mendations/ticks/ (Accessed 16 June 2012).

Asri S, Mahmoudian A (2001) Serological study of Canine Ehrlichiosis in West- ern and Estern Azarbaijan’s of Iran.

th WSAVA congress, 2001 August8 - 11, Vancouver, British Columbia, Canada.

Avize R, Mosallanejad B, Razi Jalali MH, Alborzi AR (2010) Seroprevalence of Ehrlichia canis in dogs referred to Veterinary Hospital of Shahid Chamran University of Ahvaz, Iran. Arch Razi Inst. 65: 1.

Baneth G, Breitschwerdt EB, Hegarty BC, Pappalardo B, Ryan J (1998) A sur- vey of tick-borne bacteria and proto- zoa in naturally exposed dogs from Israel. J Vet Parasitol. 74: 133-142.

Bashiribod H, Kazemi B, Eslami G, Bigdeli S, Bandehpour M, Rahbarian N, Ramezani Z (2004) First molecular detection of Anaplasma phagocytophilum in Ixodes ricinus tickes in Iran. J Med Sci. 4:282–286

Batmaz H, Nevo E, Waner T, Senturk S, Ylmaz Z, Harrus S (2001) Seropre- valence of ehrlichia canis antibodies among dogs inTurkey. Vet Rec. 148:665–666.

Botros BA, Elmolla MS, Salib AW, Calamaio CA, Dasch GA, Arthur RR (1995) Canine ehrlichiosis in Egypt: sero-epidemiological survey. Onderstepoort J Vet Res. 62: 41–43.

Ettinger SJ, Feldman EC (1995) Textbook of Veterinary Internal Medicine. 4th edn, Saunders, Philadelphia.

Breitschwerdt EB, Hegarty BC, Hancock SI (1998) Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii. J Clin Microbiol. 36: 2645–2651

Cardenas AM, Doyle CK, Zhang X, Nethery K, Corstvet RE, Walker DH, McBride JW (2007) Enzyme-linked immunosorbent assay with conserved immunoreactive glycoproteins gp36 and gp19 has enhanced sensitivity and provides species-specefic immune- diagnosis of Ehrlichia canis infection. J Clin Vaccine Immunol. 14:123–128.

Carvalho FS, Wenceslau AA, Carlos RS, Albuquerque GR (2008) Epidemio- logical and molecular study of Ehrlichia canis in dogs in Bahaia, Brazil. J Genet Mol Res. 29: 657–662.

Costa LM, Rembeck K, Ribeiro MF, Beelitz P, Pfister K, Passos LM (2007) Sero-prevalence and risk indi- cators for canine ehrlichiosis in three rural areas of Brazil. J Vet. 174: 673–676.

Dagnone AS, Trapp SM, Jojima FS, Amude AM, Morais HAS, Freire RL, Vidotto O (2002) Avaliação Soroepi- demiológica da infecção por Ehrlichia canis, Dirofilaria immitis e Borrelia burgdorferi em cães de uma população hospitalar. XII Congresso Brasileiro de Parasitologia Vete- rinária, Rio de Janeiro.

Dantas-Torres F (2008) The brown dog tick,Rhipicephalus sanguineus (Latreille 1806) (Acari: Ixodidae): from taxon- omy to control. J Vet Parasitol. 152:173–185.

Dantas-Torres F (2010) Biology and ecol- ogy of the brown dog tick, Rhipicephalus sanguineus. J Parasites and Vectors. 26: 1–10.

Donatein A, Lestoquard F (1937) State of the present knowledge concerning rickettsiosis of animals. Arch Inst Pasteur Alger 15: 142–187.

Friesen S, Goedde J, Henderson B, Syl- vester W (2008) Prevalence of Ehrlichia canis, Anaplasma platys, Babesia canis vogeli, Hepatozoon canis, Bartonella vinsonii berkhoffi, and Rickettsia spp. In dogs from Gre- nada. Vet Parasitol. 151: 279–285.

Futch RR, Corstvet RE (1996) Diagnosis Ehrlichia canis infection in dogs us- ing enzyme-linked immunosorbent assays for antibody and antigen. Pre- sented at the Meeting of the Ameri- can Association of Veterinary Labor- atory Diagnosticians, Little Rock, AR.

Graya J, Dantas-Torresb F, Estrada-Penad A, Levine M (2013) Systematics and ecology of the brown dog tick, Rhipicephalus sanguineus. Ticks Tick Borne Dis. 4: 171–180.

Groves MG, Dennis GL, Amyx HL, Huxsoll DL (1975) Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineous). Am J Vet Res. 36: 937–940.

A Silent, Deadly Killer (2004) Ehrlichiosis.Available at: http://home.earthlink.net/~hawkeye87/Ehrlichiosis%20Pag e.htm (Accessed July 2004).

Harrus S, Aroch I, Lavy E, Bark H (1997a) Clinical manifestations of infectious canine cyclic thrombocytopenia. Vet Rec. 141: 247–250.

Harrus S, Kass PH, Klement E, Waner T (1997b) Canine monocytic ehrlichiosis: a retrospective study of 100 cases, and an epidemiological investigation of prognostic indicators for the dis- ease. Vet Rec.141: 360–363.

Hernandez MB, Perez Diaz JV, Garcia SV, Garcia SV, Garcia Pena FG (2005) Comparsion of The Prevalence of The Infection by Leptospira spp, Leishmania infantum and Ehrlichia canis in Dogs in the Comunida Valencina (Spain). Epidémiol. et santé anim. 45: 83–86.

Huxsoll DL, Hildebrandt PK, Nims RM, Ferguson JA, Walker JS (1969) Ehrlichia canis-the causative agent of a haemorrhagic disease of dogs. Vet Rec. 85: 21.

Jafari S, Gaur SNS, Hashemi A (1997) Prevalence of Ehrlichia Canis in Dog Population of Shiraz,Fars Province of Iran. J Anim Res. 11: 19–23.

Jafari Shoorijeh S,Rowshan Ghasrodashti A, Tamadoni A, Moghaddar N, Behzadi MA (2008) Seasonal Fre- quency of Ectoparasite Infestion in Dogs from Shiraz, Southern Iran. Turk J Vet Anim Sci. 32: 309–313.

Inokuma H, Ohno K, Yamamoto S (1999) Serosurvey of Ehrlichia canis and Hepatozoon canis infection in dogs in Yamaguchi Prefecture Japan. J Vet Med Sci. 61: 1153–1155.

Lim S, Ahn K, Irwin PJ, Myung B, Lee SR,Oh MH, Shin SS (2010) Com- parison of selected canine vector- borne disease between urban animal shelter and rural hunting dogs in Ko- rea. Parasit Vectors. 3: 32.

University of Florida Institute of Food and Agricultural Sciences (2011) Brown dog tick, Rhipicephalus sanguineus Latreille (Arachnida: Acari: Ixodidae). Available at: http://www.entnemdept.ufl.edu/creatures/urban/medical/brown _dog_tick.htm (Accesse 15 June 2012).

Matthewman LA, Kelly PJ, Mahan SM,Semu D, Tagwira M, Bobade PA, Brouqui P, Mason PR, Raoult D (1993) Western blot and indirect fluo- rescent antibody testing for antibod- ies reactive with Ehrlichia canis in sera from apparently healthy dogs in Zimbabwe. J S Afr Vet Assoc. 64:111–115.

Matthewman LA, Kelly PJ, Bobade PA, Tagwira M, Mason PR, Majok A, Brouqui P, Raoult D (1993) Infec- tions with Babesia canis and Ehrlichia canis in dogs in Zimbabwe. J Vet Rec.133: 344–346.

Mavromatis K, Kuyler Doyle C, Lykidis A, Ivanova N, Francino MP, Chain P, Shin M, Malfatti S, Larimer F, Copeland A, Detter JC, Land M, Richardson PM, Yu XJ, Walker DH, McBride JW, Kyrpides NC (2006) The Genome of the Obligately Intra- cellular Bacterium Ehrlichia canis Reveals Themes of Complex Mem- brane Structure and Immune Evasion Strategies. J Bact. 188: 4015–4023.

Morales-Soto M, Cruz-Vazquez C (1998) Population fluction of Rhipicephalus sanguineus, the dog’s tick, in Cuernavac, Morelos in Valley. Prelim- inary study. Vet Mex. 29: 299–301.

Moreira SM, Machado RZ, Passos LF (2005) Detection of Ehrlichia canis in bone marrow aspirates of experi- mentally infected dogs. Cienc Rural.35: 958–960.

Mylonakis ME, Koutinas AF, Billinis C, Leontides LS, Kontos V, Papado- poulos O, Rallis T, Fytianou A (2003) Evaluation of cytology in the diagno- sis of acute canine monocytic ehrlichiosis (Ehrlichia canis): a com- parison between five methods. J Vet Microbiol. 91: 197–204.

Nyindo MBA, Huxsoll DL, Ristic M, Kakoma I, Brown JL, Carson CA, Stephenson EH (1980) Cell-mediated and humor immune responses of Ger- man shepherd dogs and beagles to experimental infection with Ehrlichia canis. Am J Vet Res. 42: 250–254.

Neer TM, Harrus S (2006) Canine monocytotropic ehrlichiosis and neorickettsiosis (E. canis, E. chaffeensis, E. ruminantium, N. sennetsu, and N. risticii infections). In: Greene CE (ed): Infectious diseases of the dog and cat,3rd ed. Saunders Elsevier, St. Louis, Missouri, pp. 203–216

Ploneczka K, Smielewska-Los E (2003) Prevalence of antibodies specific to Ehrlichia canis in dogs from south- west Poland. J Med Vet. 59: 1005–1008.

Perez M, Bodor M, Zhang C, Ziong Q, Rikihisa Y (2006) Human infection with Ehrlichia canis accompanied by clinical signs in Venezuela. Ann NY. Acad Sci. 1078: 110–117.

Razmi GRA, Naghibi MR, Aslani K, Dastjerdi K, Hossieni H (2003) An epidemiological study on Babesia in- fection in small ruminants in Mash- had suburb, Khorasan Province, Iran. J Small Anm Res. 50: 39–44.

Rikihisa Y (1991) The tribe Ehrlichiae and ehrlichial disease. J Clin Microbial Rev. 286–308.

Ristic M, Dawson JE, Holland CJ, Jenny A (1988) Susceptibility of dogs to in- fection with E. risticii, the causative agent of equine monocytic ehrlichiosis (Potomac horse fever). Am J Vet Res.49: 1497–1500.

Ristic M, Huxsoll DL, Weisiger RM, Hil- debrandt PK, Nyindo MBA (1972) Serological diagnosis of tropical ca- nine pancytopenia by indirect immu- nofluorescence. Infect Immun. 6: 226–231.

Rodriguez-Vivas RI, Albornz RE, Bolio GM (2005) Ehrlichia canis in dogs in Yucatan, Mexico: seroprevalence, prevalence of infection and associated factors. Vet Parasitol. 127: 75–79.

Roqueplo C, Cheminel V, Bourry O, Gomez J, Prevosto JM, Parzy D, Davoust B (2009) Canine ehrlichiosis in the Ivory Coast and Gabon: alteration of biochemical blood parameters based on Ehrlichia canis serology. J Clin Microbiol Infect Dis. 15: 41–42.

Roura X, Breitschwerdt E, Lloret A, Ferrer L, Hegarty B (2005) Serological Evi- dence of Exposure to Rickettsia, Bartonella, and Ehrlichia Species in Healthy or Leishmania infantum-In- fected Dogs from Barcelona,Spain. Intern. J Appl Res Vet Med. 3: 2.

Sacchini F, Cessford RJ, Robinson BM (2007) Outbreak of canine ehrlichiosis in Saudi Arabia. J Vet Clin Pathol.36: 331–335.

Schutze GE, Buckingham SC, Marshall GS, Woods CR, Jackson MA, Patter- son LE, Jacobs RF (2007) Tick-borne infections in children study (TICS) group. Human monocytic ehrlichiosis in children. Pediatr Infect Dis J. 26:475–479.

Siuda K (1993) Kleszcze Polski (Acari: Ixodida). Cz. II. Systematyka i rozmieszczenie. Polskie Towarzystwo Parazytologiczne. Warszawa Solano-Gallego L, Trotta M, Razia L, Furlanello T, Caldin M (2006) Mo- lecular survey of Ehrlichia canis and Anaplasma phagocytophilum from blood of dogs in Italy. AnnN Y Acad Sci. 1078: 515–518.

Suksawat J, Hegarty BC, Breitschwerdt EB (2000) Seroprevalence of Ehrlichia canis, Ehrlichia equi and Ehrlichia risticii in sick dogs from North Caro- lina and Virginia. J Vet Intern Med.14: 50–55.

Tabar MD, Francino O, Altet L, Sánchez A, Ferrer L, Roura X (2009) PCR survey of vectorborne pathogens in dogs living in and around Barcelona, an area endemic for leishmaniasiss. J Vet Rec. 164: 112–116

Trapp SM, Dagnone AS, Vidotto O, Freire RL, Amude AM, de Morais HA (2002) Seroepidemiology of canine babesiosis and ehrlichiosis in a hos- pital population in South Brazil. J Vet Intern Med. 16: 365.

Troy GC, Vulgamott JC, Turnwald GH (1980) Canine ehrlichiosis: a retro- spective study of 30 naturally occur- ring cases. J Am Anim Hosp Assoc.16:181–187.

Uspensky I, Ioffe-Uspensky I (2002) The dog factor in brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae) infestation in and near hu- man dwellings. Int J Med Microbial.291: 156–163.

Waner T, Harrus S, Bark H, Bogin E, Avidar Y, Keysary A (1996) Sub- clinical canine ehrlichiosis (Ehrlichia canis) in experimentally infected bea- gle dogs. J Am Coll Vet Intern Med.10:192.

Waner T, Strenger C, Keysary A (2000a) Comparison of a clinic-based ELISA test kit with the immunofluorescence test for the assay of Ehrlichia canis antibodies in dogs. J Vet Diagn In- vest. 12: 240–244.

William JK (1981) Military working dogs and canine ehrlichiosis (tropical canine pancytopenia) in the Vietnam War. Command and General Staff College (CGSC) MMAS thesis, US Army Command and General Staff College, Fort Leavenworth, USA.

Wikswo ME, Hu R, Metzger ME, Eremeeva ME (2007) Detection of Rickettsia rickettsii and Bartonella henselae in Rhipicephalus sanguineus Ticks from California. J Med Entomol. 44: 158–162.

Yabsley MJ, Mckibben J, Macpherson CN, Cattan PF, Cherry NA, Hegarty BC, Breitschwerdt EB, O'Connor T, Chandrashekar R, Paterson T, Perea ML, Ball G, Friesen S, Goedde J,Henderson B, Sylvester W (2008) Prevalence of Ehrlichia canis, Ana- plasma platys, Babesia canis vogeli, Hepatozoon canis, Bartonella vinsonii berkhoffii, and Rickettsia spp. in dogs from Grenada. Vet Parasitol. 151:279–278.

Published
2015-10-11
How to Cite
1.
Ansari-Mood M, Khoshnegah J, Mohri M, Rajaei S. Seroprevalence and Risk Factors of Ehrlichia canis Infection among Companion Dogs of Mashhad, North East of Iran, 2009–2010. J Arthropod Borne Dis. 9(2):184-194.
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