Environmental Factors Associated with Larval Habitats of Anopheline Mosquitoes (Diptera: Culicidae) in Metema District, Northwestern Ethiopia

  • Esayas Aklilu Department of Biology, Mada Walabu University, Bale-Robe, Ethiopia
  • Mizan Kindu Department of Parasitology, School of Medicine, Microbiology and Immunology, MadaWalabu University, Bale-Robe, Ethiopia
  • Araya Gebresilassie Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia
  • Solomon Yared Department of Biology, Jigjiga University, Jigjiga, Ethiopia
  • Habte Tekie Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia
  • Meshesha Balkew Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia
Anpheles gambiae s.l.; Larval habitat; Metema; Ethiopia


Background: Malaria is one of the major public health concerns in Ethiopia. Control options available for the manage­ment of malaria, include case detection, personal protection and larval source management. Effective control of Anoph­eles larvae largely depends on understanding of the habitats of the vectors. The aims of this study were to identify the breeding habitats of mosquitoes and characterize the larval habitats in Gende Wuha Town in northwestern Ethiopia.Methods: Different aquatic habitats were sampled and characterized for anopheline larvae from November 2012 to June 2013.Results: In total, 2784 larvae of Anopheles mosquitoes were collected from various breeding habitats. Microscopic identification of the III and IV instars revealed the presence of seven Anopheles species. Of the Anopheles spp, Anophe­les gambiae s.l. (80%) was the most predominant species in the study area. Spearman correlation coefficient results also determined that the density of An. gambiae s.l. increased significantly with habitat temperature (r= 0.346, p< 0.01). Significantly higher An. gambiae s.l. larvae were obtained in non-shaded habitats (z= -3.120, p< 0.05) when compared with shaded habitats.Conclusions: The current study demonstrated An. gambiae s.l., the principal malaria vector in the country, is the pre­dominant species in the larval sampling habitats. It was also noted the importance of edge of stream as larva breeding habitats for this species during the dry season of the year. Therefore, attention should be given for this breeding habitat for control of the vector during dry season.


1. Gilles M (2002) The malaria parasites. In: Gilles H, Warrell D (Eds): Essential Ma¬lariology. Vol. 97. Oxford Press, Lon¬don, pp. 8–34.
2. World Health Organization (2016) World Malaria Report. World Health Organi-zation, Geneva, p. 186.
3. World Health Organization (2018) World Malaria Report. World Health Organi-zation, Geneva, pp. 210.
4. Federal Minster of Health (2014) An epi-demiological profile of malaria in Ethi-opia. Federal Ministry of Health, Addis Ababa, Ethiopia.
5. Ghebreyesus A, Deressa W, Witten K, Get¬achew A, Seboxa T (2006) Malaria. In: Berhane Y, Haile-Marim D, Kloos H (Eds). Epidemiology and ecology of health and disease in Ethiopia. Shama Books, Addis Ababa, pp. 556–576.
6. Walter Reed Biosystematics Unit (2013) Sys¬tematic Catalog of Culicidae. Walter Reed Biosystematics Unit, Smith¬sonian Institution, Washington DC, USA. Available at: http://www.mosquitocatalog.org.
7. Abose T, Yebbyo Y, Olana D, Alamirew D, Yikuno Amlake B, Regassa L, Mengesha A (1998) The epidemiology and control of malaria with special em-phasis on the distribution, behavior and susceptibility of insecticides of Anophe¬line vectors and chloroquine resistance in Awash, Cen¬tral Ethiopia and other area. World Health Organization/ MAL.
8. Kenea O, Balkew M, Gebre-Michael T (2011) Environmental factors associated with larval habitats of Anopheline mos¬quitoes (Diptera: Culicidae) in Irrigation and major drainage areas in the middle course of the Rift Valley, Central Ethi¬opia. J Vector Borne Dis. 48: 85–92.
9. Overgaard HJ, Tsuda Y, Suwonkerd W, Takagi M (2002) Characteristics of Anopheles minimus (Diptera: Culicidae) larval habitats in northern Thailand. En¬viron Entomol. 31: 134–141.
10. Mwangangi JM, Mbogo CM, Muturi EJ, Nzovu JG, Githure JI, Yan G, Mina-kawa N, Novak R, Beier JC (2007) Spa-tial dis¬tribution and habitat characteriza¬tion of Anopheles larvae along the Ken¬yan coast. J Vector Borne Dis. 44: 44–51.
11. Teklu M, Tekie H, McCartney M, Kibret S (2010) The effect of physical water quality and water level changes on the occurrence and density of Anopheles mos-quito larvae around the shoreline of the Koka Reservoir, Central Ethiopia.‎ Hy-drol Earth Syst Sci. 14: 2595–2603.
12. Animut A, Gebre-Michael T, Balkew M, Lindtjørn B (2012) Abundance and dy-namics of Anopheline larvae in a high-land malarious area of south-central Ethi¬opia. Parasite Vector. 5: 117.
13. Minakawa N, Mutero CM, Githure JI, Beier JC, Yan G (1999) Spatial dis-tribu¬tion and habitat characterization of Anophe¬line mosquito larvae in western Kenya. Am J Trop Med Hyg. 61: 1010–1016.
14. Lane J (1974) The preservation and mount¬ing of insects of medical im-portance. World Health Organization, Geneva, p. 20.
15. Gillies, MT, Coetzee M (1987) A sup¬ple-ment to the Anophelinae of Africa, South of the Sahara. Publication of the South Africa Institute for Medical Re-search. Johannesburg, South Africa, p. 143.
16. Minakawa N, Githure JI, Beier JC, Yan G (2001) Anopheline mosquito survival strategies during the dry period in west-ern Kenya. J Med Entomol. 38: 388–392.
17. Imbahale S, Paaijmans K, Mukabana W, Lammeren R, Githeko A, Githeko W (2011) A longitudinal study on Anophe-les mosquito larval abundance in dis-tinct geographical and environmental settings in Western Kenya. Mal J. 10: 81–87.
18. Mala AO, Irungu LW, Shililu JI, Muturi EJ, Mbogo CC, Njagi JK, Githure JL (2011) Dry season ecology of Anophe-les gambiae complex mosquitoes at lar-val habitats in two traditionally semi-ar¬id villages in Baringo, Kenya. Parasite Vector. 25: 4.
19. Dejenie T, Yohannes M, Assmelash M (2011) Characterization of mosquito breed¬ing sites in and in the vicinity of Tigray microdams. Ethiop J Health Sci. 21: 57–66.
20. Bayoh MN, Lindsay SW (2003) Effect of temperature on the development of aquat¬ic stages of Anopheles gambiae sensu stricto (Diptera: Culicidae). Bull Ento¬mol Res. 93: 375–381.
21. Tuno N, Okeka W, Minakawa N, Takagi M, Yan G (2005) Anopheles gambiae sensu stricto (Diptera: Culicidae) larvae in western Kenya highland forest. J Med Entomol. 42: 270–277.
22. Manguin S, Roberts D, Peyton DE, Rejmanko¬va E, Pecor J (1996) Charac-terization of Anopheles pseudopunc-tipen¬nis larval habitats. J Am Mosq Control Assoc. 12: 619–625.
23. Gimnig JE, Ombok M, Otieno S, Kauf-man MG, Vulule JM, Walker ED (2001) Density-dependent development of Anoph¬eles gambiae (Diptera: Cu¬licidae) larvae in artificial habitats. J Med Entomol. 39: 162–172.
How to Cite
Aklilu E, Kindu M, Gebresilassie A, Yared S, Tekie H, Balkew M. Environmental Factors Associated with Larval Habitats of Anopheline Mosquitoes (Diptera: Culicidae) in Metema District, Northwestern Ethiopia. J Arthropod Borne Dis. 14(2):153–161.
Original Article