Molecular Epidemiology and Phylogeny of Crimean-Congo Haemorrhagic Fever (CCHF) Virus of Ixodid Ticks in Khorasan Razavi Province of Iran

  • Hossein Maghsood Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AND Iranian Research Center for Tick and Tick-Borne Diseases, University of Tehran, Tehran, Iran
  • Sedigheh Nabian Mail Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AND Iranian Research Center for Tick and Tick-Borne Diseases, University of Tehran, Tehran, Iran
  • Parviz Shayan Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AND Iranian Research Center for Tick and Tick-Borne Diseases, University of Tehran, Tehran, Iran
  • Tahmineh Jalali Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasture Institute of Iran, Tehran, Iran AND Research Center for Emerging and Reemerging Infectious Disease, Pasteur Institute of Iran, Tehran, Iran
  • Meysam Saboor Darbandi Veterinary Organization of Kalat, Khorasan Razavi, Iran
  • Mohammad Mehdi Ranjbar Department of Foot and Mouth Disease Vaccine Production, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
Crimean-Congo haemorrhagic fever; Ixodid ticks; Khorasan Razavi Province


Background: Crimean–Congo hemorrhagic fever (CCHF) is a fatal disease caused by Nairovirus classified within the Bunyaviridae family. The virus is transmitted to humans through the bites of infected ticks or direct contact with viremic animals or humans. The current study aimed to detect the virus genome in ticks from Khorasan Razavi Province.Methods: One hundred hard ticks were collected randomly from 100 sheep in four different areas of the province. Collected ticks were kept alive and identified. All the ticks were analyzed for the presence of CCHF virus genome using reverse transcriptase polymerase chain reactions (RT-PCR). Results: The identified ticks were belonging to Hyalomma marginatum (16% female and 6% male), Rhipicephalus turanicus (52% female and 25% male), and Dermacentor raskemensis (1%). The CCHF virus genome was found in Hyalomma marginatum (5% male from Taibad and Sabzevar region and 1% female from Taibad). Genetic analysis of the virus genome isolated from two regions (Sabzevar and Taibad) showed 100% identity. Conclusion: This study indicated that CCHF should be regarded as a risk-borne infection in this province. Therefore, special health management is needed to control this disease.


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How to Cite
Maghsood H, Nabian S, Shayan P, Jalali T, Saboor Darbandi M, Ranjbar MM. Molecular Epidemiology and Phylogeny of Crimean-Congo Haemorrhagic Fever (CCHF) Virus of Ixodid Ticks in Khorasan Razavi Province of Iran. J Arthropod Borne Dis. 14(4):400–407.
Original Article