Antigenic Cross-Reactivity Anti-Birtoxin Antibody against Androctonus crassicauda Venom

  • SuhandanAdigüzel Van-Zoelen Mail Netherlands Organisation for Applied Scientific Research-Innovation for life-Hollanda, Turkey.
  • Ozcan Ozkan Drug and Medical Device Agency of Turkey, Ankara, Turkey.
  • Bora Inceoglu Department of Entomology, University of California, Davis, USA.
Keywords:
Androctonus crassicauda, Venom, anti-birtoxin antibody, Cross-reactivity

Abstract

Background: Antivenom is still widely used in the treatment of envenomation as there are no vaccines or other effective agents available against animal venoms. Recently, neurotoxins named birtoxin family have been described from Parabuthus transvaalicus and Androctonus crassicauda. The aim of the present study was to test the antibirtoxinantibodies for their ability to neutralize the lethal effects of A. crassicauda scorpion venom.Methods: SDS-PAGE and Western blotting used the presence of components from A. crassicauda and P.transvaalicus scorpion venoms and to determine the degree of cross-reactivity. The Minimum Lethal Dose (MLD) of venom was assessed by subcutaneously (sc) injections in mice.Results: The MLD of the A. crassicauda venom was 35 μg/ 20g mouse by sc injection route. Western blotting showed the presence of components from A. crassicauda and P. transvaalicus scorpion venoms strongly cross react with the A. crassicauda antivenom. However, Western blotting of the A. crassicauda scorpion venom using the Refik Saydam Public Health Agency (RSPHA) generated antibody showed that not all the venom components cross reacted with the anti-birtoxin antibody. The antibodies only cross reacted with components falling under the 19 kDa protein size of A. crassicauda venom.Conclusion: The bioassays and Western blotting of A. crassicauda venom with the anti-birtoxin antibodies produced against a synthetic peptide showed that these antibodies cross reacted but did not neutralize the venom of A. crassicauda.

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Published
2015-10-11
How to Cite
1.
Van-Zoelen S, Ozkan O, Inceoglu B. Antigenic Cross-Reactivity Anti-Birtoxin Antibody against Androctonus crassicauda Venom. J Arthropod Borne Dis. 9(2):176–183.
Section
Original Article