Original Article

The Effect of Different Doses of Mesobuthus eupeus (Scorpionida: Buthidae) Scorpion Venom on the Production of Liver Necrosis in Nmri Mice


Background: Scorpion venom has a variety of different components considerably. Some of these compounds are pro­teins such as Phospholipase A2 which is one of the most important. Use of scorpion venom for the treatment of any disease requires an initial study to determine the therapeutic dose or safe dose. Therefore, due to the necessity of study­ing scorpion venom, it is of special importance to study the effects of its dose response in animal tissues.Methods: To determine the inflammatory effects of scorpion’s venom (Mesobuthus eupeus), 50 Nmri mice with an average weight of 24±7g were selected for investigation in two experiments. In first-round 25 of them were divided into 5 groups and were exposed to different doses of venom injection paralleling the control group. Single-injection of vari­ous doses on 25 mice was performed and results were compared.Results: There was a significant differences between the test and control groups (in most groups). Liver necrosis was one of the important symptoms in this study, the severity of which was measured and statistically analyzed.Conclusion: It was determined that 0.05ppm is a safe dose and sub-lethal doses can use for the investigation of thera­peutic effects of venom on cancer, diabetes, dermatitis, and so on.
1. Xie W, Xing D, Zhao Y, Su H, Meng Z, Chen Y, Du L (2005) A new tactic to treat postprandial hyper-lipidemia in di¬abetic rats with gastro paresis by im-proving gastrointestinal transit. Eur J Pharmacol. 7: 113–120.
2. Moosavi SM, Imani S, Haghighi S, Mousa¬vi SE, Karimi A (2012) Effect of Ira¬nian honey bee (Apis mellifera) venom on blood glucose and insulin in diabetic rats. J Arthropod Borne Dis. 6(2): 136–143.
3. Roodbari L (2012) Effect of Anderoctonus crassicauda venom in the treatment of type 1 diabetes in rats. [MSC disserta-tion]. Tehran Islamic Azad University, Science and Research, Iran. (In Per-sian).
4. Morgan NG, Montague W (1984) Stimula-tion of insulin secretion from isolated rat islets of Langerhans by melittin. Bi-osci Rep. 4(8): 665–671.
5. Simonsson E, Karlsson S, Ahren B (2000) Islet phospholipase A(2) activation is potentiated in insulin resistant mice. ‎Bi¬ochem Biophys Res Commun. 272(2): 539–543.
6. Fujimoto WY, Metz SA (1987) Phasic ef-fect of glucose, phospholipase A2, and lyso-phospholipids on insulin secretion. J Endocrinol. 120(5): 1750–1755.
7. Nemati M, Forouzan AR, Pouladgar A, Boroun F, Nemati A (2011) Antioxidant Effects of scorpion’s venom compo¬nents. The first regional congress on ven¬omous animals and toxin, 2011 De¬cember 1–3, Razi Vaccine and Serum Research Institute, Karaj, Iran, pp. 92–93.
8. Ghane M (2009) Stud¬ies on the identifica-tion, purification, determination of tox-icity and molecular weight of Bothotus schakh venom. [MSC dissertation]. Teh¬ran Islamic Azad University, Science and Research, Tehran, Iran (In Persian).
9. Kadkhodayi alyadorani M, Amouzgari Z, Hanifi H (2007) Phospholipase and Hy-aloronidase activity in Mesobuthus eu-peus venom. Feyz. 4: 24–30.
10. Roghani M, Bagheri A, Asyabi M, Hoda-vandkhani A (2004) Effect of the aque-ous extract of coriander leaves in dia-betic mice by STZ. Iran J Endocrinol Metab. 4: 259–255.
11. Shaikh DM, Jokhio P (2005) Effect of snake venom on nucleic acid and total proteins in various normal and cancer-ous animal tissues. Pak J Physiol. 1: 1–2.
12. Jian-Wei L, Jing H, Gui-Rong Z, Zheng-Ren W (2006) Effects of anti-cancer pep¬tide fraction 3 from Buthus Mar-tensii Karsch on apoptosis of human liver can¬cer cells. J Jilin Univ Med Ed. 32: 625–628.
13. Shi-bo F, Ying Y, Feng-qin Y, Shu-chun L, Peng-wu L, Gui-zhi J (2007) Inhibi-tory effects of fraction from scorpion venom on growth of SKOV3 ovarian car¬cinoma cells. J Jilin Univ Med Ed. 33: 41–43.
14. Shahramyar Z, ZareMirakabad A, Mo-rovat H, Lotfi M, Nouri AQ (2011) Induction of apoptosis in human Leuke-mia cell line (HL60) by animal´s venom derived peptides (ICD-85). The first re¬gional congress on venomous animals and toxin, 2011 December 1–3, Razi Vac¬cine and Serum Research Institute, Ka¬raj, Iran, pp. 174–175.
15. García AD, Díaz LM, Herrera YF, Sánchez HR, Lorenzo YC, Llanes DM, Garlobo YR, Castro JF (2013) In vitro anticancer effect of venom from Cuban scorpion Rhopalurus junceus against a panel of human cancer cell lines. J Venom Res. 4: 5–12.
IssueVol 15 No 1 (2021) QRcode
SectionOriginal Article
DOI https://doi.org/10.18502/jad.v15i1.6486
Dose-response; Liver necrosis; Nmri mice; Scorpion’s venom

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Mojdegani-Fard S, Imani S, Shojaei M. The Effect of Different Doses of Mesobuthus eupeus (Scorpionida: Buthidae) Scorpion Venom on the Production of Liver Necrosis in Nmri Mice. J Arthropod Borne Dis. 2021;15(1):60–68.