Original Article

Comparative Testing of Susceptibility Levels of Phlebotomus sergenti, the Main Vector of Anthroponotic Cutaneous Leishmaniasis, to Conventional Insecticides Using Two Capture Methods in Kerman City, Southeastern Iran


Background: Collecting live sand flies from indoor sites is a major challenge for researchers in large cities due to the reluctance of families to survey their homes. This study was conducted to assess the efficacy of two methods for col­lecting sand flies for use in susceptibility tests in the urban area of Kerman, southeastern Iran.Methods: Sandflies were mainly collected using both baited traps and hand catch methods from outdoor and indoor sites. Susceptibility tests were separately done according to the standard World Health Organization testing protocol on Phlebotomus sergenti, including 60-minute exposure to DDT 4.0%, propoxur 0.1%, deltamethrin 0.05%, and malathion 5.0%.Results: During this research, the natural habitats and suitable indoor sites were selected to predict the density of live sand fly with perfect accuracy. The number of live Ph. sergenti caught by hand catch and baited traps methods was 42 and 361 in indoor and outdoor sites, respectively. The mortality rate of Ph. sergenti exposed to DDT 4%, deltamethrin 0.05, malathion 5%, and propoxur was 100%.Conclusion: The baited traps showed a significant efficiency compared to hand catch for collecting live Ph. sergenti for use in susceptibility tests in urban areas. The Ph. sergenti collected from both indoor and outdoor sites were suscep­tible to all insecticides.
1. Javadian E (2008) Epidemiology of cutane-ous leishmaniasis in Iran. In: Nadim A, Javadian E, Momeni A (Eds) Leishmania and leishmaniasis. Iran University Press 1295, Tehran, pp. 191–211.
2. Seyedi-Rashti MA, Keighobadi K, Nadim A (1984) Urban cutaneous leishmaniasis in Kerman, southeast Iran. Bull Soc Pathol Exot. 77: 312–319.
3. Jafari S, Hajiabdolbaghi M, Mohebali M, Haj¬jaran H, Hashemian H (2010) Dis-semi¬nat¬ed leishmaniasis caused by Leishmania trop¬ica in HIV-positive pa-tients in the Islamic Republic of Iran. East Mediterr Health J. 16: 340–343.
4. Bamorovat M, Sharifi I, Mohammadi MA, Eybpoosh S, Nasibi S, Aflatoonian MR, Khosravi A (2018) Leishmania tropica isolates from non-healed and healed pa-tients in Iran: A molecular typing and phylogenetic analysis. Microb Pathog. 1 (116): 124–129.
5. Sharifi I, Fekri AR, Aflatoonian MR, Khamesipour A, Mahboudi F, Dowlati Y, Nadim A, Modabber F (2010) Leish-maniasis recidivans among school chil-dren in Bam, Southeast Iran, 1994–2006. Intern J Dermatol. 49: 557–561.
6. Bamorovat M, Sharifi I, Aflatoonian MR, Sharifi H, Karamoozian A, Sharifi F, Kar¬amoozian A, Sharifi F, Khosravi A, Has¬sanzadeh S (2018) Risk factors for anthroponotic cutaneous leishmaniasis in un¬responsive and responsive patients in a ma¬jor focus, southeast of Iran. PloS One. 13: e0192236.
7. Bamorovat M, Sharifi I, Dabiri S, Moham-madi MA, Fasihi Harandi M, Mohebali M, Aflatoonian MR, Keyhani A (2015) Leishmania tropica in stray dogs in south¬east Iran. Iran J Public Health. 44(10): 1359–1366.
8. Mohebali M, Malmasi A, Hajjaran H, Jam-shidi S, Akhoundi B, Rezaei M, Janita-bar S, Zarei H, Charehdar S (2011) Dis-seminated leishmaniasis caused by Leish¬mania tropica in a puppy from Ka-raj, central Iran. Iran J Parasitol. 6: 69–73.
9. Dereure J, Rioux JA, Gallego M, Perieres J, Pratlong F, Mahjour J, Saddiki H (1991) Leishmania tropica in Morocco: infection in dogs. Trans R Soc Trop Med Hyg. 85(5): 595–599.
10. Guilvard E, Rioux JA, Gallego M, Prat-long F, Mahjour J, Martinez-Ortega E, Dereure J, Saddiki A, Martini A (1991) Leishma¬nia tropica in Morocco. III iden¬tification of 89 isolates from the vector Phlebotomus sergenti. Ann Para-sitol Hum Comp. 66: 96–99.
11. Yahia H, Ready PD, Hamdani A, Testa JM, Guessous N (2004) Regional dif-feren¬ti¬ation of Phlebotomus sergenti in three Moroccan foci of cutaneous leishmaniasis cause by Leishmania tropica. Parasite. 11: 189–199.
12. Izri A, Bendjaballah A, Andriantsoanirina V, Durand R (2014) Cutaneous leish-man¬iasis caused by Leishmania killicki, Algeria. Emerg Infect Dis. 20(3): 502–504.
13. Amro A, Gashout A, Al-Dwibe H, Za-hang¬ir Alam M, Annajar B, Hamarsheh O (2012) First Molecular Epidemiologi-cal Study of Cutaneous Leishmaniasis in Lib¬ya. PLoS Negl Trop Dis. 6(6): e1700.
14. Tabbabi A (2019) Review of Leishmania-sis in the Middle East and North Africa. Afr Health Sci. 19(1): 1329–1337.
15. Tabbabi A, Ghrab J, Aoun K, Ready PD, Bouratbine A (2011) Habitats of the sand¬fly vectors of Leishmania tropica and L. major in a mixed focus of cuta-neous leish¬maniasis in South East Tuni-sia. Acta Trop. 119: 131–137.
16. Nadim A, Rostami GS (1974) Epidemiol-ogy of cutaneous leishmaniasis in Ka¬bul, Afghanistan. Bull World Health Org. 51: 45–49.
17. Nadim A, Javadian E, Noushin MK, Na¬yil AK (1979) Epidemiology of cutane¬ous leishmaniasis in Afghanistan. Part II. An¬throponotic cutaneous leishmania¬sis. Bull Soc Pathol Exot Filiales. 72: 461–466.
18. Javadian E, Nadim A, Nayil AK (1982) Ep¬idemiology of cutaneous leishmania-sis in Afghanistan. Part III. Notes on sandflies of Afghanistan. Bull Soc Pathol Exot Fil¬iales. 75: 284–290.
19. Reithinger R, Mohsen M, Aadil K, Sidiqi M, Erasmus P, Coleman PG (2003) Anthroponotic cutaneous leishmaniasis, Ka¬bul, Afghanistan. Emerg Infect Dis. 9: 727–729.
20. Hewitt S, Reyburn H, Ashford R, Row-land M (1998) Anthroponotic cutaneous leish¬maniasis in Kabul, Afghanistan: ver¬tical distribution of cases in apart-ment blocks. Trans R Soc Trop Med Hyg. 92: 273–274.
21. Reyburn H, Rowland M, Mohsen M, Khan B, Davies C (2003) The pro¬longed epidemic of anthroponotic leishmaniasis in Kabul, Afghanistan: ‘bringing down the neighborhood'. Trans R Soc Trop Med Hyg. 97: 170–176.
22. Shirzadi MR, Gouya MM (2010) Na¬tion¬al Guidelines for Cutaneous Leish¬maniasis Surveillance in Iran. Ministry of Health and Medical Education, Zo¬onoses Control Department, Tehran, Iran.
23. Hussain M, Munir S, Jamal MA, Ayaz S, Akhoundi M, Mohamed K (2017) Epi-demic outbreak of anthroponotic cuta-ne¬ous leishmaniasis in Kohat District, Khy¬ber Pakhtunkhwa, Pakistan. Acta Trop. 172: 147–155.
24. El-Beshbishy HA, Al-Ali KH, El-Badry AA (2013) Molecular characterization of Leishmania infection in sand flies from Al-Madinah Al-Munawarah Province, West¬ern Saudi Arabia. Exp Parasitol. 134: 211–215.
25. Muhjazi G, Gabrielli AF, Ruiz-Postigo JA, Atta H, Osman M, Bashour H, Al Tawil A, Husseiny H, Allahham R, Al¬lan R (2019) Cutaneous leishmaniasis in Syria: A review of available data during the war years: 2011–2018. PLoS Negl Trop Dis. 13(12): e0007827.
26. Kamhawi S, Abdel-Hafez SK, Arbagi A (1995) A new focus of cutaneous leish-maniasis caused by Leishmania tropica in northern Jordan. Trans R Soc Trop Med Hyg. 89(3): 255–257.
27. Salam N, Al-Shaqha WM, Azzi A (2014) Leishmaniasis in the Middle East: inci-dence and epidemiology. PLoS Negl Trop Dis. 8(10): e3208.
28. Svobodova M, Votypka J, Peckova J, Dvo¬rak V, Nasereddin A, Baneth G, Sztern J, Kravchenko V, Orr A, Meir D, Schnur LF, Volf P, Warburg A (2006) Distinct transmission cycles of Leish-mania tropica in 2 adjacent foci. Emerg Infect Dis. 12: 1860–1868.
29. Zeyrek FY, Korkmaz M, Ozbel Y (2007) Serodiagnosis of anthroponotic cutane-ous leishmaniasis (ACL) caused by Leishma¬nia tropica in Sanliurfa Prov-ince, Turkey, where ACL is highly en-demic. Clin Vaccine Immunol. 14: 1409–14015.
30. Nadim A, Javadian E, Seyedi- Rashti MA (1994) Epidemiology of leishmaniasis in Iran. In: Ardehali S, Rezaei R, Nadim A (Eds) Leishmania Parasite and leish¬man-iasis. University Publishing Center, Teh-ran, pp. 176–208.
31. Nadim A, Seyedi-Rashti MA, Mesghali A (1971) Epidemiology of cutaneous leish-man¬iasis in Iran. B. Khorassan. Part IV. Distribution of sand flies. Bull Soc Path Exot. 64(6): 865–870.
32. Nadim A, Tahvildari-Bidruni GH (1977) Epidemiology of cutaneous leishmania-sis in Iran: B. Khorassan, Part VI: Cuta-neous leishmaniasis in Neishabur, Iran. Bull Soc Pathol Exot. 70: 171–177.
33. Ghatee MA, Mirhendi H, Marashifard M, Kanannejad Z, Taylor WR, Sharifi I (2018) Population structure of Leishma-nia tropica causing anthroponotic cuta-neous leishmaniasis in southern Iran by PCR-RFLP of kinetoplastid DNA. Bi-omed Res Int. 2018: 6049198.
34. Yaghoobi-Ershadi MR (1977) Studies on Cutaneous Leishmaniasis in the City of Kerman. [MSPH Thesis]. University of Tehran, Iran.
35. Sharifi I, Aflatoonian MR, Fekri AR, Ha-kimi Parizi M, Aghaei-Afshar A, Khosra¬vi A (2015) A comprehensive review of cutaneous leishmaniasis in Kerman Prov¬ince, southeastern Iran. Narrative review article. Iran J Public Health. 44: 299–307.
36. Yaghoobi-Ershadi MR, Hanafi-Bojd AA, Javadian E, Jafari R, Zahraei-Ramazani AR, Mohebali M (2002) A new focus of cutaneous leishmaniasis caused by Leish¬mania tropica. Saudi Med J. 23: 291–294.
37. Nadim A, Aflatoonian MR (1995) Anthro¬ponotic cutaneous leishmaniasis in Bam, southeast Iran. Iran J Public Health. 24: 15–24.
38. Aflatoonian MR, Sharifi I, Nadim A, Af-latoonian B (2014) Comparison of the pe¬riod prevalence of urban cutaneous leish¬maniasis (CL) in Bam in two time periods of 1990–1992 and 2010–2012. Iran J Public Health. 9: 32–39.
39. Sharifi I, Fekri AR, Aflatoonian MR, Nadim A, Nikian Y, Khamesipour A (1998) Cu¬taneous leishmaniasis in pri-mary school children in the southeastern Iranian city of Bam, 1994–1995. Bull World Health Org. 76: 289–293.
40. Sharifi I, Nakhaei N, Aflatoonian MR, Ha¬kimi Parizi M, Fekri AR, Safizadeh H, Shirzadi MR, Goya MM, Khamesipour A (2011) Cutaneous leishmaniasis in Bam: A comparative evaluation of pre- and post- earthquake years (1999–2008). Iran J Public Health. 40: 49–56.
41. Zahraei-Ramazani A, Yaghoobi-Ershadi MR, Mokhtari A, Akhavan AA, Abdoli H, Arandian M (2007) Anthroponotic cu¬taneous leishmaniasis in nonendemic quar¬ters of a central city in Iran. Iran J Public Health. 36(2): 7–11.
42. Mirzaei M, Sharifi I, Poursmaelian S (2012) A new focus of anthroponotic cutaneous leishmaniasis and identifica-tion of parasite species by nested PCR in Jiroft, Iran. Comp Clin Pathol. 21(5): 1071–1075.
43. Yaghoobi-Ershadi MR (2012) Phlebotom-ine sand flies (Diptera: Psychodidae) in Iran and their role on Leishmania trans-mission. J Arthropod Borne Dis. 6(1): 1–17.
44. Mesghali A, Seyedi-Rashti MA, Nadim A (1967) Epidemiology of cutaneous leish-maniasis in Iran. II. Natural lep¬tomonad infection of sand flies in the Mashhad and Lotfabad areas. Bull Soc Pathol Exot. 60: 514–517.
45. Mozafary M, Dayer MS, Aghaei Afshar A, Mollaie HR (2016) Epidemiology of cutaneous leishmaniasis and molecular characterization of its causative agents in naturally infected sand flies in en-dem¬ic foci of Kerman City, southeastern Iran. Asian Pac J Trop Dis. 6(3): 188–192.
46. Aghaei Afshar A, Rassi Y, Sharifi I, Vatan¬doost H, Mollaie HR, Oshaghi MA, Abai MR, Rafizadeh S (2014) First report on natural Leishmania infection of Phleboto¬mus sergenti due Leishma-nia tropica by high resolution melting curve method in southeastern Iran. Asian Pac J Trop Med. 7(2): 93–96.
47. Azizi K, Askari MB, Kalantari M, Moemenbel¬lah-Fard MD (2016) Molec-ular detection of Leishmania parasites and host blood meal identification in wild sand flies from a new endemic ru-ral region, south of Iran. Pathog Glob Health. 110: 303–309.
48. Moemenbellah-Fard MD, Ahmadyousefi-Sarhadi M, Azizi K, Fakoorziba MR, Kalanta¬ri M, Amin M (2015) Faunal iden¬tification and frequency distribution of wild sand flies infected with Leish-mania tropica. Asian Pac J Trop Dis. 5(10): 792–797.
49. Oshaghi MA, Rasolian M, Shirzadi MR, Mohtarami F, Doosti S (2010) First re-port on isolation of Leishmania tropica from sand flies of a classical urban cu-taneous leishmaniasis focus in southern Iran. Exp Parasitol. 126: 445–450.
50. Al-Zahrani MA, Peters W, Evans DA, Ching C, Smith IV, Lane RP (1988) Phleboto¬mus sergenti, a vector of Leishmania trop¬ica in Saudi Arabia. Trans R Soc Trop Med Hyg. 82: 416.
51. Tabbabi A, Bousslimi N, Rhim A, Aoun K, Bouratbine A (2011) First report on nat¬ural infection of Phlebotomus ser-genti with Leishmania promastigotes in the cu¬taneous leishmaniasis focus in southeast¬ern Tunisia. Am J Trop Med Hyg. 85: 646–647.
52. Aghasi M, Sharifi I (2003) Survey of the fauna and monthly activity of the sand fly as the vectors of the cutaneous leish-maniasis in the city of Bam. J Kerman Univ Med Sci. 10(2): 85–91.
53. Mirhoseini M, Salehzadeh A, Ramazan Jamaat S, Zahirnia A H, Rahmanzadeh N (2017) Distribution and seasonal ac¬tiv¬ity of Phlebotominae sand flies in Yazd and its outskirts, center of Iran. Sci World J. 1486845.
54. Nadim A, Seyedi-Rashti MA (1991) Some aspects to the ecology of Phlebotomus sergenti in Iran. First In¬ternational Sym-posium on Phlebotom¬ine Sand flies Rome, Italy, p. 79.
55. Fakoorziba MR, Nazari M (2006) Ento¬mo-logical studies of Phlebotomus pa¬patasi and Ph. sergenti (Diptera: Psy¬chodidae) as vectors of cutaneous leishmaniasis in Shiraz, Iran. Southeast Asian J Trop Med Public Health. 37 Suppl 3: 115–117.
56. Yaghoobi-Ershadi MR, Hakimiparizi M, Zahraei-Ramazani AR, Abdoli H, Akhavan AA, Aghasi M, Arandian MH, Ranjbar AA (2010) Sand fly surveil-lance within an emerging epidemic fo-cus of cutaneous leishmaniasis in south-eastern Iran. Iran J Arthropod Borne Dis. 4(1): 17–23.
57. Zahraei-Ramazani AR, Akhavan AA, Abdoli H, Jafari R, Jalalizand AR, Aran¬dian MH, Shareghi N, Ghanei M (2008) Some ecological aspects of phlebotominae sandflies (Diptera: Psy-chodidae) in an endemic focus of an-throponotic cutaneous leishmaniasis of Iran. J Entomol. 5(1): 17–23.
58. Hmamouch A, El Alem MM, Hakkour M, Amarir F, Daghbach H, Habbari K, Fellah H, Bekhti K, Sebti F (2017) Cir-cu¬lating species of Leishmania at mi¬cro-cli¬mate area of Boulemane Province, Morocco: impact of environmental and hu¬man factors. Parasite Vectors. 10(1): 100.
59. Ullah K, Khan NH, Sepúlveda N, Munir A, Wahid S (2016) Assessing incidence patterns and risk factors for cutaneous leishmaniasis in Peshawar region, Khy-ber Pakhtunkhwa, Pakistan. J Parasitol. 102: 501–506.
60. Aflatoonian MR, Sharifi I, Parizi MH, Fekri AR, Aflatoonian B, Sharifi M, Khosravi A, Khamesipour A, Sharifi H (2014) A prospective cohort study of cutaneous leishmaniasis risk and opium addiction in south eastern Iran. PLoS One. PMID: 24586494.
61. Desjeux P (2001) The increase in risk factors of leishmaniasis worldwide. Trans R Soc Trop Med Hyg. 95: 239–245.
62. Dujardin JC (2006) Risk factors in the spread of leishmaniases: towards inte-grated mon¬itoring? Trends Parasitol. 22: 4–6.
63. Aflatoonian MR, Sharifi I, Poursmaelian S, Hakimi-Parizi M, Ziaali N (2013) The emer¬gence of anthroponotic cuta¬neous leishmaniasis following the earthquake in southern villages of Bam District, south¬eastern Iran, 2010. J Ar¬thropod Borne Dis. 7(1): 8–14.
64. Aflatonian MR, Montazeri F, Jalali M, Nadim A, Sharifi F (2006) The status of the disease in Bam in 2005 years and the comparison with the last five years (before and after the earthquake). J Kerman Uni Med Sci. 13: 37–43.
65. Sharifi I, Poursmaelian S, Aflatoonian MR, Fotouhi Ardakani R, Mirzaei M, Fekri AR, Khamesipour A, Hakimi Parizi M, Fasihi Harandi M (2011) Emergence of a new focus of anthro-ponotic cutaneous leishmaniasis due to Leishmania tropica in rural communi¬ties of Bam District after the earthquake, Iran. Trop Med Intern Health. 16(4): 510–513.
66. Fazaeli A, Fouladi B, Sharifi I (2009) Emer¬gence of cutaneous leishmaniasis in a bor¬der area at south-east of Iran: an epidemiological survey. J Vector Borne Dis. 46: 36–42.
67. Alawieh A, Musharrafieh U, Jaber A, Berry A, Ghosn N, Bizri AR (2014) Re-vis¬iting leishmaniasis in the time of war: the Syrian conflict and the Leba¬nese outbreak. Int J Infect Dis. 29: 115–119.
68. Alasaad S (2013) War diseases revealed by the social media: massive leishmani¬asis outbreak in the Syrian Spring. Para¬site Vectors. 6: 94.
69. Inci R, Ozturk P, Mulayim MK, Ozyurt K, Alatas ET, Inci MF (2015) Effect of the Syrian civil war on prevalence of cutaneous leishmaniasis in southeastern Anatolia, Turkey. Medical science monitor. Intl Med J Exp Clin Res. 21: 2100–2104.
70. Kolaczinski J, Brooker S, Reyburn H, Row¬land M (2004) Epidemiology of anthroponotic cutaneous leishmaniasis in Afghan refugee camps in northwest Pakistan. Tran Roy Soc Trop Med Hyg. 98: 373–378.
71. Rowland M, Munir A, Durrani N, Noyes H, Reyburn H (1999) An outbreak of cu¬taneous leishmaniasis in an Afghan refugee settlement in north-west Paki-stan. Trans R Soc Trop Med Hyg. 93: 133–136.
72. Saroufim M, Charafeddine K, Issa G (2014) Ongoing epidemic of cutaneous leishman¬iasis among Syrian refugees, Lebanon. Emerg Infect Dis. 20(10): 1712–1715.
73. Brooker S, Mohammed N, Adil K, Agha S, Reithinger R, Rowland M, Ali I, Ko-laczinski J (2004) Leishmaniasis in ref-ugee and local Pakistani populations. Emerg Infect Dis. 10(9): 1681–1684.
74. His Excellency, Murshidi MM, Hijjawi MQ, Jeriesat S, Eltom A (2013) Syrian refugees and Jordan's health sector. Lancet. 382(9888): 206–207.
75. Du R, Hotez PJ, Al-Salem WS, Acosta-Ser¬rano A (2016) Old world cutaneous leish¬maniasis and refugee crises in the Middle East and North Africa. PLoS Negl Trop Dis. 10(5): e0004545.
76. Saroufim M, Charafeddine K, Issa G, Kha¬lifeh H, Habib RH, Berry A, Ghosn N, Rady A, Khalifeh I (2014) Ongoing epidemic of cutane¬ous leishmaniasis among Syrian refu¬gees, Lebanon. Emerg Infect Dis. 20(10): 1712–1715.
77. Seyedi-Rashti MA, Nadim A (1975) Re-es¬tablishment of cutaneous leishmania¬sis af¬ter cessation of anti-malaria spraying. Trop Geogr Med. 27(1): 79–82.
78. World Health Organization (2010) Con¬trol of the Leishmaniasis. Report of a meeting of the WHO export committee on the control of leishmaniasis, WHO Tech¬nical Report Series 949, Geneva.
79. Yaghoobi-Ershadi MR (2016) Control of phlebotomine sand flies in Iran: a re¬view article. J Arthropod Borne Dis. 10(4): 429–444.
80. González U, Pinart M, Sinclair D (2015) Vector and reservoir control for pre¬vent-ing leishmaniasis. Cochrane Data¬base Syst Rev. published by John Wiley and Sons, Ltd. on behalf of The Cochrane Collaboration. p. 80.
81. Maroli M, Khoury C (2004) Prevention and control of leishmaniasis vectors: current approaches. Parasitologia. 46: 211–215.
82. Nadim A, Motabar M, Houshmand B, Key¬ghobadi K, Aflatoonian MR (1995) Eval¬uation of Pyrethroid Impregnated Bed Nets for Control of Anthroponotic Cuta¬neous Leishmaniasis in Bam (Is-lamic Re¬public of Iran). WHO, Geneva, Switzerland. Available at: https://apps.who.int/iris/handle/10665/61138.
83. Yaghoobi-Ershadi MR, Moosa-Kazemi SH, Zahraei-Ramazani AR, Jalaizand AR, Akha¬van AA, Arandian MH (2006) Evaluation of deltamethrin impregnated bed nets and curtains for control of zo¬onotic cutaneous leishmaniasis in hy¬peren¬demic area of Iran. Bull Soc Pathol Exot. 99(1): 43–48.
84. Kolaczinski JH, Muhammad N, Khan QS, Jan Z, Rehman N (2004) Subsi¬dized sales of insecticide-treated nets in Afghan ref¬ugee camps demonstrate the feasibility of a transition from humani-tarian aid towards sustainability. Malar J. 3: 15.
85. Courtenay O, Gillingwater K, Gomes PA, Garcez LM, Davies CR (2007) Del¬tame-thrin-impregnated bednets reduce human landing rates of sandfly vector Lutzomyia longipalpis in Amazon households. Med Vet Entomol. 21(2): 168–176.
86. Jalouk L, Al Ahmed M, Gradoni L, Mar¬oli M (2007) Insecticide-treated bed nets to prevent anthroponotic cutaneous leishman¬iasis in Aleppo Governorate, Syria: results from two trials. Trans R Soc Trop Med Hyg. 101(4): 360–367.
87. Kroeger A, Avila EV, Morison L (2002) Insecticide impregnated curtains to control domestic transmission of cuta-neous leishmaniasis in Venezuela: cluster randomized trial. BMJ. 325(7368): 810–813.
88. Moosa-Kazemi SH, Yaghoobi-Ershadir MR, Akhavan AA, Abdoli H, Zahraei-Ram¬azani AR (2007) Deltamethrin-im-preg¬nated bed nets and curtains in an anthro¬ponotic cutaneous leishmaniasis control program in northeastern Iran. Ann Saudi Med. 27: 6–12.
89. Reyburn H, Ashford R, Mohsen M, Hewitt S, Rowland M (2000) A ran-domized con¬trolled trial of insecticide-treated bednets and chaddars or top sheets, and residual spraying of interior rooms for the prevention of cutaneous leishmaniasis in Ka¬bul, Afghanistan. Trans R Soc Trop Med Hyg. 94: 361–366.
90. Aghaei-Afshar A, Vatandoost H, Sharifi I, Mollaie H, Oshaghi MA (2013) First de¬termination of impact and outcome indicators following indoor residual spraying (IRS) with deltamethrin in a new focus of Anthroponotic Cutaneous Leishmaniasis (ACL) in Iran. Asian Pac J Trop Dis. 3: 5–9.
91. World Health Organization (1981) In-struc¬tions for determining the suscepti-bility or resistance of blackflies, sand flies and biting midges to insecticides. WHO/ VBC/81.810.
92. World Health Organization (1986) Re-sistance of vectors and reservoirs of dis-ease to pesticides. 10th report of the WHO Expert committee. Vector Biol-ogy and Con¬trol. Tech Rep Ser, p. 737.
93. World Health Organization (2016) Test pro¬cedures for insecticide resistance monitoring in malaria vector mosqui¬toes, II. WHO, Geneva. Available at: http://apps.who.int/iris/bitstre am/10665/250677/1/9789241511575-eng.pdf (Accessed on August 15, 2017).
94. Nadim A, Javadian E (1976) Key for spe-cies identification of sandflies (Phlebotom¬inae: Diptera) of Iran. Iran J Public Health. 5: 33–44.
95. Javadian E, Mesghali A (1975) Checklist of phlebotomine sand flies (Diptera: Psy¬cho¬didae) of Iran. Bull Soc Pathol Exot. 68: 207–209.
96. Rassi Y, Hanafi-Bojd AA (2006) Phlebotom¬ine Sand Flies, Vectors of Leishmaniasis: Morphology, biology, ecology, and field and laboratory meth-ods with pictorial key of Iranian sand flies. Noavaran-Elm Publication, Teh¬ran, Iran.
97. Hesam-Mohammadi M, Rassi Y, Abai MR, Akhavan AA, Karimi F, Rafizadeh S, Sanei-Dehkordi A, Sharafkhah M (2014) Efficacy of different sampling methods of sand flies (Diptera: Psycho-didae) in en¬demic focus of cutaneous leishmaniasis in Kashan District, Isfahan Province, Iran. J Arthropod Borne Dis. 8(2): 156–162.
98. Arzamani K, Rassi Y, Vatandoost H, Akha¬van AA, Abai MR, Alavinia M, Akbarzadeh K, Mohebali M, Rafizadeh S, Karim¬ian F, Badakhshan M, Absava-ran A (2019) Comparative Performance of different traps for collection of phlebotom¬inae sand flies and estimation of biodiversity indices in three endemic leishman¬iasis foci in North Khorasan Province, Northeast of Iran. J Arthropod Borne Dis. 13(4): 399–406.
99. Kasap ÖE, Belen A, Kaynas S, Simsek FM, Biler L, Ata N, Alten B (2009) Ac-tivity patterns of sand fly (Diptera: Psy-cho¬di¬dae) species and comparative per-formance of different traps in an en-demic cutaneous leishmaniasis focus in Cukurova Plain, Southern Anatolia, Turkey. Acta Vector Borne. 78(2): 327–335.
100. Hoel DF, Kline DL, Hogsette JA, Ber-nier UR, El-Hossary SS, Hanafi HA, Watany N, Fawaz EY, Furman BD, Obenauer PJ, Szumlas DE (2010) Effi-cacy of commer¬cial mosquito traps in capturing Phlebotom¬ine sand Flies (Diptera: Psychodidae) in Egypt. J Med Entomol. 47 (6): 1179–1184.
101. Aghasi M (1996) Present Status of An-throponotic Cutaneous Leishmaniasis in Kerman, Southeast Iran. [MSPH thesis]. School of Public Health, Tehran Uni¬ver-sity of Medical Sciences, Tehran, Iran.
102. Moosa-Kazemi SH, Yaghoobi-Ershadi MR, Akhavan AA, Abdoli H, Zahraei-Rama¬zani AR, Jafari R, Houshmand B, Nadim A, Hosseini M (2007) Deltamethrin-im¬pregnated bednets and curtains in an anthroponotic cutaneous leishmaniasis control program in northeastern Iran. Ann Saudi Med. 27(1): 6–12.
103. Afshar AA, Rassi Y, Sharifi I, Abai MR, Oshaghi M, YaghoobiErshadi MR, Vatan¬doost H (2011) Susceptibility status of Phlebotomus papatasi and Ph. sergenti (Diptera: Psychodidae) to DDT and deltamethrin in a focus of cutaneous leishmaniasis after earthquake strike in Bam, Iran. J Arthropod Borne Dis. 5(2): 32–41.
104. Arzamani K, Vatandoost H, Rassi Y, Abai MR, Akhavan AA, Alavinia M, Akbarzadeh K, Mohebali M, Rafizadeh S (2017) Susceptibility status of wild population of Phlebotomus sergenti (Diptera: Psychodidae) to different ima-gicides in a en¬demic focus of cutaneous leishmaniasis in northeast of Iran. J Vector Borne Dis. 54: 282–286.
105. Tetreault GE, Zayed AEBB, Hanafi-Bojd AA, Beavers GM, Zeichner BC (2001) Sus¬ceptibility of sandflies to selected in¬sec¬ticides in North Africa and the Mid¬dle East. J Am Mosq Control Assoc. 17 (1): 23–27.
106. Faraj C, Ouahabi S, Adlaoui EB, El Elkohli M, Lakraa L, El Rhazi M, Ameur B (2012) Insecticide suscepti-bility status of Phleboto¬mus (Paraph-lebotomus) sergenti and Phlebotomus (Phlebotomus) papatasi in endemic foci of cutaneous leishmaniasis in Morocco. Parasite Vectors. 5: 51.
IssueVol 15 No 1 (2021) QRcode
SectionOriginal Article
Phlebotomus sergenti; Susceptibility; Insecticides; Baited traps; Hand catch

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How to Cite
Rassi Y, Ebrahimi S, Abai MR, Vatandoost H, Akhavan AA, Aghaie Afshar A. Comparative Testing of Susceptibility Levels of Phlebotomus sergenti, the Main Vector of Anthroponotic Cutaneous Leishmaniasis, to Conventional Insecticides Using Two Capture Methods in Kerman City, Southeastern Iran. J Arthropod Borne Dis. 15(1):82-96.