The Effect of Hemiscorpius lepturus (Scorpionida: Hemiscorpiidae) Venom on Leukocytes and the Leukocyte Subgroups in Peripheral Blood of Rat

  • Mehri Ghafourian Health Research Institute, Research Center of Thalassemia and Hemoglobinopathy, Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Neda Ganjalikhanhakemi Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Ali Asghar Hemmati Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • Rouhullah Dehghani Department of Environmental Health, School of Health and Social Determinants of Health (SDH), Research Center, Kashan University of Medical Sciences, Kashan, Iran
  • Wesam Kooti Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
Keywords:
Hemiscorpius lepturus, Venom, Leucocyte, Rat

Abstract

Background: The aim of this study was to investigate the effect of Hemiscorpius lepturus venom on leukocytes and the leukocyte subgroups in peripheral blood of rat.Methods: In this experimental study, sixty N-Mari rats were divided into three groups of 20 rats. Then the rats in each group were divided into four subgroups based on the blood sampling time that was 2, 6, 24 and 48 hours after the venom injection, respectively. The control group did not receive anything, however, the first and the second ex­perimental groups received 0.1 and 0.01mg/kg of venom, subcutaneously. In accordance with a designated four sam­pling times, the blood sampling was carried out in three groups. After RBC lysis, the leukocytes and leukocyte sub­populations were determined and counted using appropriate hematological standard methods.Results: The leukocyte and the neutrophil count at two (P<0.05), six (P<0.01) and 24 (P<0.05) hours after the venom injection showed a significant decline compared with the control group, this decrease was significant at the dose of 0.1 mg/kg until 48 hours after the venom injection (P<0.05). The lymphocyte count showed a significant decline throughout the all hours of the experiment, compared with the control group (P<0.05).Conclusion: Leukocytes are probably affected by the cytotoxicity effect of the H. lepturus venom in a dose-dependent manner. This could be a wakeup call for the medical staff to perform quick and accurate treatment in the least time possible.

References

Abbas AK, Lichtman HH, Pillai S (2011) Cellular and Molecular Immunology.7th ed. PA: Elsevier Health Sciences. Philadelphia.

Bigdeli MR, Taghavi-Moghadam A (2006) Extraction of proteinous components from the venom of scorpion Hemiscorpious lepturus and evaluation of some their toxic effects in mice. Jundishapur Sci Med J. 5(2): 538–542.

Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the prin- ciple of protein-dye binding. Anal Bi- ochem. 72: 248–254.

Celis A, Gaxiola-Robles R, Sevilla-Godinez E, de Orozco Valerio MJ, Armas J (2007) Trends in mortality from scor- pion stings in Mexico, 1979–2003. Rev Panam Salud Publica. 21(6): 373–380.

Chippaux JP, Goyffon M (2008) Epidemiol- ogy of scorpionism: a global appraisal. Acta Trop. 107(2): 71–79.

Chitnis PA, Maraghi Sh, Vazirianzadeh B (1993) Epidemiological and laboratory study on scorpion stings in Khuzestan Province. J Med Fac Guilan Univ Med Sci. 2: 5–12.

Dehghani R, Valayi N (2005) Review on scorpions’ taxonomy and Iranian scor- pions’ key identification. Feiz. 32: 73–92.

Dehghni R, Dinparast Djadid N, Shahbazzadeh D, Bigdelli S (2009) Introducing Compsobuthus matthiesseni (Birula,1905) scorpion as one of the major stinging scorpions in Khuzestan, Iran. Toxicon. 54: 272–275.

Dehghani R, Fathi B (2012) Scorpion sting in Iran: a review. Toxicon. 60(5): 919–933.

Dehghani R, Khamehchian T, Vazirianzadeh B, Vatandoost H, Moravvej SA (2012) Toxic effects of scorpion, Hemiscorpius lepturus (Hemiscorpiidae) venom on mice. J Anim Plant Sci. 22(3): 593–596.

Dehghani R, Khamehchian T, Tirgari S, Vatandoost H, Rasi Y, Rafeenejad J (2005) Study of the Effect of Hemiscorpius lepturus Venom on Levels of WBC, RBCc and Hematocrit in Rats. J Shaheed Sadoughi Univ Med Sci. 13(1): 66–70.

Jalali A, Pipelzadeh MH, Taraz M, Khodadadi A, Makvandi M, Rowan EG (2011) Serum TNF-α levels reflect the clinical severity of envenomation following a Hemiscorpius lepturus sting. Eur Cy- tokine Netw. 22(1): 5–10.

Jean-Philippe Ch (2012) Emerging options for the management of scorpion stings. Drug Des Dev Ther. 6: 165–173.

Khodadadi A, Pipelzadeh MH, Vazirianzadeh V, Pipelzadeh M, Sharifat M (2012) An in vitro comparative study upon the toxic properties of the venoms from Hemiscorpius lepturus, Androctonu- scrassicauda and Mesobuthuseupeus scorpions. Toxicon. 60(3): 385–390.

Khoobdel M, Zahraei-Salehi T, Nayeri- Fasaei B, Khosravi M, Omidian Z, Motedayen M, Akbari A (2013) Puri- fication of the Immunogenic Fractions and Determination of Toxicity in Mes- obuthus eupeus (Scorpionida: Buthi- dae) Venom. J Arthropod-Borne Dis.7(2): 139–146.

Mahbod A (2008) Principles, Practical App-lication and Interpretation of Tests in hematology. 4th Ed. Eshraghiye Pub, Tehran.

Ghafourian Boroujerdnia M, Mohebby Z (2008) A survey on comparison of Hemiscorpius lepturus and black scor- pion effects on peripheral blood sub- populations of patients with scorpion bite under 15 years old referred to Ahwaz Abuzar Hospital. Toxicol Lett.180: 131.

Mansouri E, Kooti W, Bazvand M, Ghasemi Boroon M, Amirzargar A, Afrisham R, Afzalzadeh MR, Ashtary-Larky D, Jalali N (2015) The Effect of Hydro- Alcoholic Extract of Foeniculum vulgare Mill on Leukocytes and Hematological Tests in Male Rats. Jundishapur J Nat Pharm Prod. 10(1): e18396.

Mir Dehghan MM, Motlagh ME, Chomeili B (2001) A 5-year study of scorpion stings in children referred to Abuzar Pediatric Center in Ahwaz, 1994–1999. Urmia Med J. 12(2): 138–174.

Heidarpour M, Ennaifer E, Ahari H, Srairi- Abid N, Borchani L, Khalili Gh (2011) Histopathological changes induced by Hemiscorpius lepturus scorpion venom in mice. Toxicon. 3(59): 373–378.

Osnaya-Romero N, de Jesus Medina-Hernán- dez T, Flores-Hernández SS, León- Rojas G (2001) Clinical symptoms ob- served in children envenomated by scorpion stings, at the children's hospi- tal from the State of Morelos, Mexico. Toxicon. 39(6): 781–785.

Pipelzadeh MH, Jalali A, Taraz M, Pourabbas R, Zaremirakabadi A (2007) An epi- demiological and clinical study on scorpionism by the Iranian scorpion Hemiscorpius lepturus. Toxicon. 50(7):984–992.

Pipelzadeh M, Dezfulian A, Jalali M, Mansouri A (2006) In vitro and in vivo studies on some toxic effects of the venom from Hemiscorpious lepturus scorpion. Toxicon. 48: 93–103.

Radmanesh, M (1998) Cutaneus manifestation of Hemiscorpius lepturus sting: a cli- nical study. Int J Dermatol. 37: 500–507.

Radmansh M (1990) Clinical study of emiscorpion lepturus in Iran. J Trop Med Hyg. 93: 327–332.

Rafizadeh S, Rafinejad J, Rassi Y (2013) Epidemiology of Scorpionism in Iran during 2009. J Arthropod-Borne Dis.7(1): 66–70.

Seyedian R, Pipelzadeh MH, Jalali A, Kim E, Lee H, Kang Ch (2010) Enzymatic analysis of Hemiscorpius lepturus scor- pion venom using zymography and venom specific antivenin. Toxicon. 56 (4): 521–525.

Shahbazzadeh D, Srairi-Abid N, Feng W, Ram N, Borchani L, Ronjat M (2007) Hemicalcin a new toxin from the Ira- nian scorpion Hemiscorpius lepturus which is active on ryanodine-sensitive Ca2+ channels. Biochem J. 404: 89–96.

Shayesteh AA, Zamiri N, Peymani P, Zargani FJ, Lankarani KB (2011) A novel management method for disseminated intravascular coagulation like syndrome after a sting of Hemiscorpius lepturus: a case series. Trop Biomed. 28(3): 518–523.

Taraz M (2008) Study of serum levels of cy- tokines IL-1β, IL-6, IL-8 and TNF-α in Hemiscorpius lepturus envenomed chil- dren referred to Ahwaz City hospitals. [MD Dissertation]. School of Phar- macy, Ahwaz Jondishapur University of Medical Sciences, Iran.

Valavi E, Alemzadeh Ansari MJ (2008) He- molytic uremic syndrome following Hemiscorpius lepturus (scorpion) sting. Indian J Nephrol. 18(4): 166–168.

Vodjgani M (2012) Immunology. 8th ed. Ja- had aneshgahi Publication, Tehran.

Published
2016-05-05
How to Cite
1.
Ghafourian M, Ganjalikhanhakemi N, Hemmati AA, Dehghani R, Kooti W. The Effect of Hemiscorpius lepturus (Scorpionida: Hemiscorpiidae) Venom on Leukocytes and the Leukocyte Subgroups in Peripheral Blood of Rat. J Arthropod Borne Dis. 10(2):159-167.
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Original Article