Preparation of a Nanoemulsion of Essential Oil of Acroptilon repens Plant and Evaluation of Its Larvicidal Activity agianst Malaria Vector, Anopheles stephensi
-
Samira Firooziyan
,
Mahmoud Osanloo
,
Seyed Hassan Moosa-Kazemi
,
Hamid Reza Basseri
,
Habib Mohammadzadeh Hajipirloo
,
Ali Sadaghianifar
,
Amir Amani
,
Mohammad Mehdi Sedaghat
Abstract
Background: Extensive use of chemical larvicides to control larvae, has led to resistance in vectors. More efforts have been conducted the use of natural products such as plant essential oils and their new formulations against disease vectors. Nanoformulation techniques are expected to reduce volatility and increase larvicidal efficacy of essential oils. In this study for the first time, a larvicide nanoemulsion from the essential oil of Acroptilon repens was developed and evaluated against Anopheles stephensi larvae under laboratory conditions.
Methods: Fresh samples of A. repens plant were collected from Urmia, West Azarbaijan Province, Iran. A clevenger type apparatus was used for extracting oil. Components of A. repens essential oil (AEO) were identified by gas chromatography–mass spectrometry (GC–MS). All larvicidal bioassay tests were performed according to the method recommended by the World Health Organization under laboratory condition. Particle size and the morphologies of all prepared nanoformulations determined by DLS and TEM analysis.
Results: A total of 111 compounds were identified in plant. The LC50 and LC90 values of AEO calculated as 7 ppm and 35 ppm respectively. AEO was able to kill 100% of the larvae in 4 days.
Conclusion: The nanoemulsion of AEO showed a weak effect on the larvar mortality. It may therefore be suggested that this kind of nanoemulsion is not appropriate for the formulation as a larvicide. It is important to screen native plant natural products, search for new materials and prepare new formulations to develop alternative interventions with a long-lasting impact.
1. WHO. Global plan for insecticide resistance
management in malaria vectors: World Health
Organization; 2012.
2. Beier JC, Keating J, Githure JI, Macdonald
MB, Impoinvil DE, Novak RJ. Integrated
vector management for malaria control. Malar J.
2008;7(S1):S4.
3. Ward RD. Service MW: Medical entomology for
students. Springer; 2008.
4. Barati M, Khoshdel A, Salahi-Moghaddam
A. An overview and mapping of Anopheles in
Iran. Paramedical Sciences and Military Health.
2015;10(1):9-16.
5. Ferreira MU, Castro MC. Challenges for malaria
elimination in Brazil. Malar J. 2016;15(1):1-18.
6. Vatandoost H, Oshaghi M, Abaie M, Shahi M,
Yaaghoobi F, Baghaii M, et al. Bionomics of
Anopheles stephensi Liston in the malarious area
of Hormozgan province, southern Iran, 2002.
Acta Trop. 2006;97(2):196-203.
7. Manouchehri A, Javadian E, Eshighy N, Motabar
M. Ecology of Anopheles stephensi Liston in
southern Iran. Trop Geogr Med. 1976;28(3):228-
32.
8. Service M. A guide to Medical Entomology
(Macmillan tropical and subtropical Medical
Texts). Macmillan International College editions
London, Basingstoke; 1980.
9. Organization WH. World malaria report 2015:
World Health Organization; 2016.
10. Osanloo M, Sedaghat MM, Sanei-Dehkordi
A, Amani A. Plant-derived essential oils; their
larvicidal properties and potential application
for control of Mosquito-Borne diseases. GMJ.
2019;8:1532.
11. Firooziyan S, Amani A, Osanloo M, MoosaKazemi SH, Basseri HR, Hajipirloo HM, et al.
Preparation of nanoemulsion of Cinnamomum
zeylanicum oil and evaluation of its larvicidal
activity against a main malaria vector Anopheles
stephensi. J. environ. health sci. 2021:1-10.
12. Regnault-Roger C, Vincent C, Arnason JT.
Essential oils in insect control: low-risk products
in a high-stakes world. Annu Rev Entomol.
2012;57:405-24.
13. Isman MB, Machial CM. Pesticides based on
plant essential oils: from traditional practice to
commercialization. Advances in phytomedicine.
2006;3:29-44.
14. Pavela R. Essential oils for the development of
eco-friendly mosquito larvicides: a review. Ind.
Crops Prod. 2015;76:174-87.
15. Singh G, Kapoor IP, Pandey SK, Singh UK, Singh
RK. Studies on essential oils: part 10; antibacterial
activity of volatile oils of some spices. Phytother
Res. 2002 Nov;16(7):680-2.
16. Edris AE. Pharmaceutical and therapeutic
potentials of essential oils and their individual
volatile constituents: a review. Phytother Res.
2007;21(4):308-23.
17. Sugumar S, Clarke S, Nirmala M, Tyagi B,
Mukherjee A, Chandrasekaran N. Nanoemulsion
of eucalyptus oil and its larvicidal activity against
Culex quinquefasciatus. Bull Entomol Res.
2014;104(3):393-402.
18. Osanloo M, Amani A, Sereshti H, Abai MR,
Esmaeili F, Sedaghat MM. Preparation and
optimization nanoemulsion of Tarragon
(Artemisia dracunculus) essential oil as effective
herbal larvicide against Anopheles stephensi. Ind
Crop Prod. 2017;109:214-9.
19. Osanloo M, Sedaghat M, Sereshti H, Rahmanian
M, Saeedi Landi F, Amani A. Chitosan
nanocapsules of tarragon essential oil with low
cytotoxicity and long-lasting activity as a green
nano-larvicide. J Nanostruct. 2019;9(4):723-35.
20. Volpato A, Baretta D, Zortéa T, Campigotto G,
Galli GM, Glombowsky P, et al. Larvicidal and
insecticidal effect of Cinnamomum zeylanicum
oil (pure and nanostructured) against mealworm
(Alphitobius diaperinus) and its possible
environmental effects. J Asia Pac Entomol.
2016;19(4):1159-65.
21. Balasubramani S, Rajendhiran T, Moola AK,
Diana RKB. Development of nanoemulsion from
Vitex negundo L. essential oil and their efficacy of
antioxidant, antimicrobial and larvicidal activities
(Aedes aegypti L.). Environ Sci Pollut Res Int.
2017;24(17):15125-33.
22. Toolabi R, Abai MR, Sedaghat MM, Vatandoost
H, Shayeghi M, Tavakoli S, et al. Larviciding
activity of Acroptilon repens extract against
Anopheles stephensi, Culex pipiens and Culex
quinquefaciatus under laboratory conditions.
Pharmacogn J. 2018;10(3).
23. Organization WH. Guidelines for laboratory and
field testing of mosquito larvicides. World Health
Organization; 2005.
24. Finney DJ. Probit analysis, Cambridge University
Press. Cambridge, UK. 1971.
25. Guan H, Chi D, Yu J, Li H. Dynamics of residues
from a novel nano-imidacloprid formulation in
soyabean fields. Crop Prot. 2010;29(9):942-6.
26. Enayati A, Hemingway J. Malaria management:
past, present, and future. Annu Rev Entomol.
2010;55:569-91.
27. Zhao DB, Zhang W, Li MJ, Liu XH. [Studies
on chemical constituents of Acroptilon repens].
Zhongguo Zhong Yao Za Zhi. 2006; 31(22):1869-
72.
28. Mirza M, Shahmir F, Nik ZB. Chemical
composition of essential oil from Acroptilon
repens (L.) DC. Flavor Fragr J. 2005;20(6):615-6.
29. Norouzi‐Arasi H, Yavari I, Chalabian F,
Kiarostami V, Ghaffarzadeh F, Nasirian A.
Chemical constituents and antimicrobial activities
of the essential oil of Acroptilon repens (L.) DC.
Flavor Fragr J. 2006;21(2):247-9.
30. Nadaf M, Nasrabadi M, Halimi M, Yazdani Z,
Javanshir A, Ramazani S, et al. Identification of
non-polar chemical compounds Acroptilon repens
growing in Iran by GC-MS. Middle East J Sci
Res. 2013;17:590-2.
31. Tunalier Z, Candan NT, Demirci B, Baser KHC.
The essential oil composition of Acroptilon
repens (L.) DC. of Turkish origin. Flavor Fragr J.
2006;21(3):462-4.
32. Duarte JL, Amado JR, Oliveira AE, Cruz RA,
Ferreira AM, Souto RN, et al. Evaluation
of larvicidal activity of a nanoemulsion of
Rosmarinus officinalis essential oil. Rev Bras
Framacogn. 2015;25(2):189-92.
33. Vatandoost H, Dehkordi AS, Sadeghi S, Davari
B, Karimian F, Abai M, et al. Identification of
chemical constituents and larvicidal activity of
Kelussia odoratissima Mozaffarian essential oil
against two mosquito vectors Anopheles stephensi
and Culex pipiens (Diptera: Culicidae). Exp
Parasitol. 2012;132(4):470-4.
34. Kale NJ, Allen Jr LV. Studies on microemulsions
using Brij 96 as surfactant and glycerin, ethylene
glycol and propylene glycol as cosurfactants. Int J
Pharm. 1989;57(2):87-93.
35. Anjali C, Sharma Y, Mukherjee A,
Chandrasekaran N. Neem oil (Azadirachta
indica) nanoemulsion—a potent larvicidal agent
against Culex quinquefasciatus. Pest Manag Sci.
2012;68(2):158-63.
36. Osanloo M, Sereshti H, Sedaghat MM, Amani
A. Nanoemulsion of Dill essential oil as a green
and potent larvicide against Anopheles stephensi.
Environ Sci Pollut Res. 2018;25(7):6466-73.
37. Rodrigues EdC, Ferreira AM, Vilhena JC,
Almeida FB, Cruz RA, Florentino AC, et al.
Development of a larvicidal nanoemulsion with
Copaiba (Copaifera duckei) oleoresin. Rev Bras Farmacogn. 2014;24(6):699-705.
38. Ghosh V, Mukherjee A, Chandrasekaran N.
Formulation and characterization of plant
essential oil based nanoemulsion: evaluation of
its larvicidal activity against Aedes aegypti. Asian
J Chem. 2013;25(Supplementary Issue):S321.
39. Hajiakjoondi A, Aghel N, Xamanizadeh-Nadgar
N, Vatandoost H (2008) Chemical and biological
study of Mentha spi-cata L. essential oil from
Iran. Daru. 8 (1–2): 19–21.
40. Vatandoost H, MoinVaziri V (2004) Larvicidal
activity of a neem tree extract (Neemarin) against
mosquito larvae in the Islamic Republic of Iran.
East Mediterr Health J. 10(4/5): 573–581.
41. Sedaghat MM, Sanei-Dehkhordi AR, Khanavi
M, Abai MR, Hadjiiakhondi A, Mohtarami
F, Vatandoost H (2010) Phytochemistry and
larvicidal activity of Eucalyptus camaldulensis
against malaria vector, Anopheles stephensi.
Asian Pacific J Trop Med. 3(11): 841–845.
42. Sedaghat MM, Sanei-Dehkordi AR, Khanavi M,
Abai MR, Mohtarami F, Vatandoost H (2010)
Chemical composition and larvicidal activity of
essential oil of Cupressus arizona E.L. Greene
against malaria vector Anopheles stephensi Liston
(Diptera: Culicidae). Pharmacognosy Res. 3(2):
135–139.
43. Sanei-Dehkordi A, Vatandoost H, Abai MR, Davari
B, Sedaghat M (2016) Chemical composition and
larvicidal activity of Bunium persicum essential
oil against two important mosquito vectors. J
Essent Oil-Bear Plants. 19(2): 49–357.
44. Torabi Pour H, Shayeghi M, Vatandoost H, Abai
MR (2016) Study on larvicidal effects of essential
oils of three Iranian native plants against larvae
of Anopheles stephensi (Liston). Vector Biol J. 1
(2): 2–6.
45. World Health Organization. (2006). Pesticides
and their application for the control of vectors and
pests of public health importance, pp125.
management in malaria vectors: World Health
Organization; 2012.
2. Beier JC, Keating J, Githure JI, Macdonald
MB, Impoinvil DE, Novak RJ. Integrated
vector management for malaria control. Malar J.
2008;7(S1):S4.
3. Ward RD. Service MW: Medical entomology for
students. Springer; 2008.
4. Barati M, Khoshdel A, Salahi-Moghaddam
A. An overview and mapping of Anopheles in
Iran. Paramedical Sciences and Military Health.
2015;10(1):9-16.
5. Ferreira MU, Castro MC. Challenges for malaria
elimination in Brazil. Malar J. 2016;15(1):1-18.
6. Vatandoost H, Oshaghi M, Abaie M, Shahi M,
Yaaghoobi F, Baghaii M, et al. Bionomics of
Anopheles stephensi Liston in the malarious area
of Hormozgan province, southern Iran, 2002.
Acta Trop. 2006;97(2):196-203.
7. Manouchehri A, Javadian E, Eshighy N, Motabar
M. Ecology of Anopheles stephensi Liston in
southern Iran. Trop Geogr Med. 1976;28(3):228-
32.
8. Service M. A guide to Medical Entomology
(Macmillan tropical and subtropical Medical
Texts). Macmillan International College editions
London, Basingstoke; 1980.
9. Organization WH. World malaria report 2015:
World Health Organization; 2016.
10. Osanloo M, Sedaghat MM, Sanei-Dehkordi
A, Amani A. Plant-derived essential oils; their
larvicidal properties and potential application
for control of Mosquito-Borne diseases. GMJ.
2019;8:1532.
11. Firooziyan S, Amani A, Osanloo M, MoosaKazemi SH, Basseri HR, Hajipirloo HM, et al.
Preparation of nanoemulsion of Cinnamomum
zeylanicum oil and evaluation of its larvicidal
activity against a main malaria vector Anopheles
stephensi. J. environ. health sci. 2021:1-10.
12. Regnault-Roger C, Vincent C, Arnason JT.
Essential oils in insect control: low-risk products
in a high-stakes world. Annu Rev Entomol.
2012;57:405-24.
13. Isman MB, Machial CM. Pesticides based on
plant essential oils: from traditional practice to
commercialization. Advances in phytomedicine.
2006;3:29-44.
14. Pavela R. Essential oils for the development of
eco-friendly mosquito larvicides: a review. Ind.
Crops Prod. 2015;76:174-87.
15. Singh G, Kapoor IP, Pandey SK, Singh UK, Singh
RK. Studies on essential oils: part 10; antibacterial
activity of volatile oils of some spices. Phytother
Res. 2002 Nov;16(7):680-2.
16. Edris AE. Pharmaceutical and therapeutic
potentials of essential oils and their individual
volatile constituents: a review. Phytother Res.
2007;21(4):308-23.
17. Sugumar S, Clarke S, Nirmala M, Tyagi B,
Mukherjee A, Chandrasekaran N. Nanoemulsion
of eucalyptus oil and its larvicidal activity against
Culex quinquefasciatus. Bull Entomol Res.
2014;104(3):393-402.
18. Osanloo M, Amani A, Sereshti H, Abai MR,
Esmaeili F, Sedaghat MM. Preparation and
optimization nanoemulsion of Tarragon
(Artemisia dracunculus) essential oil as effective
herbal larvicide against Anopheles stephensi. Ind
Crop Prod. 2017;109:214-9.
19. Osanloo M, Sedaghat M, Sereshti H, Rahmanian
M, Saeedi Landi F, Amani A. Chitosan
nanocapsules of tarragon essential oil with low
cytotoxicity and long-lasting activity as a green
nano-larvicide. J Nanostruct. 2019;9(4):723-35.
20. Volpato A, Baretta D, Zortéa T, Campigotto G,
Galli GM, Glombowsky P, et al. Larvicidal and
insecticidal effect of Cinnamomum zeylanicum
oil (pure and nanostructured) against mealworm
(Alphitobius diaperinus) and its possible
environmental effects. J Asia Pac Entomol.
2016;19(4):1159-65.
21. Balasubramani S, Rajendhiran T, Moola AK,
Diana RKB. Development of nanoemulsion from
Vitex negundo L. essential oil and their efficacy of
antioxidant, antimicrobial and larvicidal activities
(Aedes aegypti L.). Environ Sci Pollut Res Int.
2017;24(17):15125-33.
22. Toolabi R, Abai MR, Sedaghat MM, Vatandoost
H, Shayeghi M, Tavakoli S, et al. Larviciding
activity of Acroptilon repens extract against
Anopheles stephensi, Culex pipiens and Culex
quinquefaciatus under laboratory conditions.
Pharmacogn J. 2018;10(3).
23. Organization WH. Guidelines for laboratory and
field testing of mosquito larvicides. World Health
Organization; 2005.
24. Finney DJ. Probit analysis, Cambridge University
Press. Cambridge, UK. 1971.
25. Guan H, Chi D, Yu J, Li H. Dynamics of residues
from a novel nano-imidacloprid formulation in
soyabean fields. Crop Prot. 2010;29(9):942-6.
26. Enayati A, Hemingway J. Malaria management:
past, present, and future. Annu Rev Entomol.
2010;55:569-91.
27. Zhao DB, Zhang W, Li MJ, Liu XH. [Studies
on chemical constituents of Acroptilon repens].
Zhongguo Zhong Yao Za Zhi. 2006; 31(22):1869-
72.
28. Mirza M, Shahmir F, Nik ZB. Chemical
composition of essential oil from Acroptilon
repens (L.) DC. Flavor Fragr J. 2005;20(6):615-6.
29. Norouzi‐Arasi H, Yavari I, Chalabian F,
Kiarostami V, Ghaffarzadeh F, Nasirian A.
Chemical constituents and antimicrobial activities
of the essential oil of Acroptilon repens (L.) DC.
Flavor Fragr J. 2006;21(2):247-9.
30. Nadaf M, Nasrabadi M, Halimi M, Yazdani Z,
Javanshir A, Ramazani S, et al. Identification of
non-polar chemical compounds Acroptilon repens
growing in Iran by GC-MS. Middle East J Sci
Res. 2013;17:590-2.
31. Tunalier Z, Candan NT, Demirci B, Baser KHC.
The essential oil composition of Acroptilon
repens (L.) DC. of Turkish origin. Flavor Fragr J.
2006;21(3):462-4.
32. Duarte JL, Amado JR, Oliveira AE, Cruz RA,
Ferreira AM, Souto RN, et al. Evaluation
of larvicidal activity of a nanoemulsion of
Rosmarinus officinalis essential oil. Rev Bras
Framacogn. 2015;25(2):189-92.
33. Vatandoost H, Dehkordi AS, Sadeghi S, Davari
B, Karimian F, Abai M, et al. Identification of
chemical constituents and larvicidal activity of
Kelussia odoratissima Mozaffarian essential oil
against two mosquito vectors Anopheles stephensi
and Culex pipiens (Diptera: Culicidae). Exp
Parasitol. 2012;132(4):470-4.
34. Kale NJ, Allen Jr LV. Studies on microemulsions
using Brij 96 as surfactant and glycerin, ethylene
glycol and propylene glycol as cosurfactants. Int J
Pharm. 1989;57(2):87-93.
35. Anjali C, Sharma Y, Mukherjee A,
Chandrasekaran N. Neem oil (Azadirachta
indica) nanoemulsion—a potent larvicidal agent
against Culex quinquefasciatus. Pest Manag Sci.
2012;68(2):158-63.
36. Osanloo M, Sereshti H, Sedaghat MM, Amani
A. Nanoemulsion of Dill essential oil as a green
and potent larvicide against Anopheles stephensi.
Environ Sci Pollut Res. 2018;25(7):6466-73.
37. Rodrigues EdC, Ferreira AM, Vilhena JC,
Almeida FB, Cruz RA, Florentino AC, et al.
Development of a larvicidal nanoemulsion with
Copaiba (Copaifera duckei) oleoresin. Rev Bras Farmacogn. 2014;24(6):699-705.
38. Ghosh V, Mukherjee A, Chandrasekaran N.
Formulation and characterization of plant
essential oil based nanoemulsion: evaluation of
its larvicidal activity against Aedes aegypti. Asian
J Chem. 2013;25(Supplementary Issue):S321.
39. Hajiakjoondi A, Aghel N, Xamanizadeh-Nadgar
N, Vatandoost H (2008) Chemical and biological
study of Mentha spi-cata L. essential oil from
Iran. Daru. 8 (1–2): 19–21.
40. Vatandoost H, MoinVaziri V (2004) Larvicidal
activity of a neem tree extract (Neemarin) against
mosquito larvae in the Islamic Republic of Iran.
East Mediterr Health J. 10(4/5): 573–581.
41. Sedaghat MM, Sanei-Dehkhordi AR, Khanavi
M, Abai MR, Hadjiiakhondi A, Mohtarami
F, Vatandoost H (2010) Phytochemistry and
larvicidal activity of Eucalyptus camaldulensis
against malaria vector, Anopheles stephensi.
Asian Pacific J Trop Med. 3(11): 841–845.
42. Sedaghat MM, Sanei-Dehkordi AR, Khanavi M,
Abai MR, Mohtarami F, Vatandoost H (2010)
Chemical composition and larvicidal activity of
essential oil of Cupressus arizona E.L. Greene
against malaria vector Anopheles stephensi Liston
(Diptera: Culicidae). Pharmacognosy Res. 3(2):
135–139.
43. Sanei-Dehkordi A, Vatandoost H, Abai MR, Davari
B, Sedaghat M (2016) Chemical composition and
larvicidal activity of Bunium persicum essential
oil against two important mosquito vectors. J
Essent Oil-Bear Plants. 19(2): 49–357.
44. Torabi Pour H, Shayeghi M, Vatandoost H, Abai
MR (2016) Study on larvicidal effects of essential
oils of three Iranian native plants against larvae
of Anopheles stephensi (Liston). Vector Biol J. 1
(2): 2–6.
45. World Health Organization. (2006). Pesticides
and their application for the control of vectors and
pests of public health importance, pp125.
| Files | ||
| Issue | Vol 15 No 3 (2021) | |
| Section | Original Article | |
| DOI | https://doi.org/10.18502/jad.v15i3.9821 | |
| Keywords | ||
| Acroptilon repens; Nanoemulsion; Larvicidal effect; Vector control; Anopheles stephensi | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Firooziyan S, Osanloo M, Moosa-Kazemi SH, Basseri HR, Hajipirloo HM, Sadaghianifar A, Amani A, Sedaghat MM. Preparation of a Nanoemulsion of Essential Oil of Acroptilon repens Plant and Evaluation of Its Larvicidal Activity agianst Malaria Vector, Anopheles stephensi. J Arthropod Borne Dis. 2021;15(3):333-346.
