Evaluation of Nanoemulsion of Eucalyptus globulus Oil as Potent Botanical Larvicide Against Malaria Vector , Anopheles stephensi and West Nile Vector, Culex pipiens Under Laboratory and Semi-field Conditions
-
Sara Sabzalizade
,
Amir Amani
,
Hassan Vatandoost
,
Fatemeh Hosseini
,
Roya Najafi-Taher
,
Hamid Reza Basseri
Abstract
Objectives Due to undesired environmental impact of insecticides as well as resistant of vectors to them, development of organic and natural insecticides have been more considered. In the current study, we developed nanoemulsion of eucalyptus and investigated lavicidal activity of it against malaria vector, Anopheles stephensi and Culex pipiens under laboratory as well as semi field conditions.
Materials and Methods: An optimized nanoemulsion was prepared by mixing Eucalyptus oil, Tween 80 and ethanol at ratio of 1:2:1.5 in distilled water, then, stirred for 20 minute at room temperature. The product was then used for bioassay tests against 3-4th instar larvae of Anopheles stephensi as well as Culex pipiens. Furthermore, semi field trial was carried out to evaluate larvicidal activity of nanoemulsion of eucalyptus.
Results: Nanoemulsion of eucalyptus showed significantly high lavicidal activity comparing with bulk eucalyptus essential oil. The LC50 and LC90 value of nanoemulsion against An. stephensi were 111.0 and 180.8 ppm respectively and 29.5 and 73.7 ppm for Cx. pipiens, respectively. In the semi field condition, the Nanoemulsion of eucalyptus decreased 1-2nd instar larval density of Culicines and Anophelines to 90.1% and 85.2%, respectively.
Conclusion: The nano formulation of eucalyptus oil showed high larvicidal activity. Therefore, nanoemulsion of eucalyptus oil can be used as an eco-friendly larvicide against mosquitoes.
1. Lajeunesse MJ, Avello DA, Behrmann MS,Buschbacher TJ, Carey K, Carroll J, Chafin TJ,Elkott F, Faust AM, Fauver H (2020) Infectedmosquitoes have altered behavior to repellents:A systematic review and meta-analysis. J Med
Entomol. 57:542-550.
2. Nadim SS, Ghosh I, Martcheva M, ChattopadhyayJ (2020) Impact of venereal transmission on the dynamics of vertically transmitted viral diseasesamong mosquitoes. Math Biosci. 325: 108366.
3. Stoops CA, Qualls WA, Nguyen TT, Richards SL(2019) A review of studies evaluating insecticidebarrier treatments for mosquito control from1944 to 2018. Environ Health Insights. 13,1178630219859004.
4. Sukumar K, Perich MJ, Boobar LR (1991)Botanical derivatives in mosquito control: areview. J Am Mosq Control Assoc. 7: 210-237.
5. Tamarina NA (1975) Natural control of bloodsuckingmosquito population densities (review ofliterature). Med Parazitol (Mosk). 44: 603-607.
6. Ochoa SA, Sanchez-Torres LE, Nevarez-Moorillon GV, Camacho AD, Nogueda-Torres B(2017) Essential oils and their components as analternative in the control of mosquito vectors ofdisease. Biomedica. 37:224-243.
7. Fallatah SA, Khater EI (2010) Potential of medicinal plants in mosquito control. J Egypt Soc Parasitol. 40:1-26.
8. Park JH, Lee HS (2018) Toxicities of Eucalyptus dives oil, 3-Carvomenthenone, and its analoguesagainst stored-product insects. J Food Prot. 81:653-658.
9. Batish DR, PalSing H, Kumar Kohli R, Kaura S (2008) Eucalyptus essential oil as a natural pesticide. For Ecol Manag.256: 2166-2174.
10. Juan LW, Lucia A, Zerba EN, Harrand L, Marco M, Masuh HM (2011) Chemical composition and fumigant toxicity of the essential oils from 16species of Eucalyptus against Haematobia irritans (Diptera: Muscidae) adults. J Econ Entomol. 104: 1087-1092.
11. Mossi AJ, Astolfi V, Kubiak G, Lerin L, Zanella C, Toniazzo G, Oliveira D, Treichel H, Devilla IA, Cansian R, Restello R (2011) Insecticidal andrepellency activity of essential oil of Eucalyptussp. against Sitophilus zeamais Motschulsky
(Coleoptera, Curculionidae). J Sci FoodAgric.91: 273-277.
12. Alzogaray RA, Lucia A, Zerba EN, Masuh HM (2011) Insecticidal activity of essential oils from eleven Eucalyptus spp. and two hybrids: lethal and sublethal effects of their major componentson Blattella germanica. J Econ Entomol. 104:595-600.
13. Anjali CH, Sharma Y, Mukherjee A,Chandrasekaran N (2012) Neem oil (Azadirachtaindica) nanoemulsion--a potent larvicidal agentagainst Culex quinquefasciatus. Pest Manag Sci.68: 158-163.
14. Salvia-Trujillo L, Soliva-Fortuny R, Rojas-Grau MA, McClements DJ, Martin-Belloso O (2017) Edible Nanoemulsions as Carriers of Active Ingredients: A Review. Annu Rev Food Sci Technol. 8: 439-466.
15. Kumar M, Bishnoi RS, Shukla AK, Jain CP (2019)Techniques for formulation of nanoemulsion drugdelivery system: A review. Prev Nutr Food Sci.24: 225-234.
16. Sarheed O, Shouqair D, Ramesh K, Khaleel T, Amin M, Boateng J, Drechsler M (2020)Formation of stable nanoemulsions by ultrasoundassisted two-step emulsification process for topical drug delivery: Effect of oil phase composition and surfactant concentration and loratadine as ripening inhibitor. Int J Pharm. 576: 118952.
17. Wang, L, Mutch KJ, Eastoe J, Heenan RK, Dong J (2008) Nanoemulsions prepared by a two-step low-energy process. Langmuir. 24: 6092-6099.
18. Kumar S, Nehra M, Dilbaghi N, Marrazza G,Hassan AA, Kim KH (2019) Nano-based smart pesticide formulations: Emerging opportunities for agriculture. J Control Release, 294, 131-153.
19. World Health Organization (2005) Guidelines for laboratory and field testing of mosquito larvicides.WHO/CDS/WHOPES/GCDPP/2005.13. pp39
20. Finney DJ (1971) Probit Analysis. 3rd ed.Cambridge University press, London.pp 333
21. Azarian-Hamidi S, Harbach RE (2009). Keys to the adult females and fourth-instar larvae of the mosquitoes of Iran (Diptera: Culicidae). Zootaxa, 2078(1), 1–33.
22. Yassin M, Ton J, Rolfe SA, Valentine TA, Cromey M, Holden N, Newton AC (2021) The rise, fall and resurrection of chemical-induced resistanceagents. Pest Manag Sci. 10.1002/ps.6370
23. Gurusubramanian G, Rahman A, Sarmah M,Ray S, Bora S (2008) Pesticide usage pattern in tea ecosystem, their retrospects and alternativemeasures. J Environ Biol. 29: 813-826.
24. Casida JE (2016) Unexpected metabolic reactionsand secondary targets of pesticide action. J Food Chem. 64: 4471-4477.
25. Smith RF, Calvert DJ (1976) Health-related aspects of integrated pest management. Environ Health Perspect. 14: 185-191.
26. Jaoko V, Nji Tizi Taning C, Backx S, MulatyaJ, Van den Abeele J, Magomere T, Olubayo F, Mangelinckx S, Werbrouck SPO, Smagghe G (2020) The phytochemical composition of Melia volkensii and its potential for insect pest management. Plants (Basel). 9(2): 143-150.
27. Casida JE (2012) The greening of pesticideenvironment interactions: some personal observations. Environ Health Perspect. 120: 487-493.
28. Chaudhari AK, Singh VK, Kedia A, Das S, Dubey NK (2021) Essential oils and their bioactive compounds as eco-friendly novel green pesticides for management of storage insect pests: prospects and retrospects. Environ Sci Pollut Res Int. 28:18918-18940.
29. Luiz de Oliveira J, Ramos Campos EV, Fraceto LF (2018) Recent developments and challenges for nanoscale formulation of botanical pesticides for use in sustainable agriculture. J Agric Food Chem. 66: 8898-8913.
30. Tian Y, Dong F, Zhou X, Yang X (2020) Repellent,insecticidal and antimicrobial activities of leaf essential oils from three eucalyptus species. ChemBiodivers. 17:e1900580.
31. Magesa SM, Kamugisha ML (2006) Evaluation of the bio-efficacy of three brands of repellents against wild populations of anthropophilic mosquitoes. Tanzan Health Res Bull. 8: 145-148.
32. Cheng SS, Huang CG, Chen YJ, Yu JJ, Chen WJ,Chang ST (2009) Chemical compositions and larvicidal activities of leaf essential oils from two eucalyptus species. Bioresour Technol. 100: 452-456.
33. Senthil Nathan S (2007) The use of Eucalyptus tereticornis Sm. (Myrtaceae) oil (leaf extract) as a natural larvicidal agent against the malaria vector Anopheles stephensi Liston (Diptera: Culicidae).Bioresour Technol. 98: 1856-1860.
34. Kweka EJ, Mosha F, Lowassa A, Mahande AM,Kitau J, Matowo J, Mahande MJ, Massenga CP,Tenu F, Feston E. Lyatuu E, Mboya M, Mndeme R, Chuwa G, Temu E (2008) Ethnobotanical study of some of mosquito repellent plants in north-eastern Tanzania. Malar J. 7:152.
35. Perugini L, Cinelli G, Cofelice M, Ceglie A, Lopez F, Cuomo F (2018) Effect of the coexistence of sodium caseinate and Tween 20 as stabilizers of food emulsions at acidic pH. Colloids Surf B Biointerfaces.168: 163-168.
36. Gonzalez A, Gutierrez-Cutino M, Moenne A (2014) Oligo-carrageenan kappa-induced reducing redox status and increase in TRR/TRX activities promote activation and reprogramming of terpenoid metabolism in Eucalyptus trees.Molecules.19: 7356-7367.
Entomol. 57:542-550.
2. Nadim SS, Ghosh I, Martcheva M, ChattopadhyayJ (2020) Impact of venereal transmission on the dynamics of vertically transmitted viral diseasesamong mosquitoes. Math Biosci. 325: 108366.
3. Stoops CA, Qualls WA, Nguyen TT, Richards SL(2019) A review of studies evaluating insecticidebarrier treatments for mosquito control from1944 to 2018. Environ Health Insights. 13,1178630219859004.
4. Sukumar K, Perich MJ, Boobar LR (1991)Botanical derivatives in mosquito control: areview. J Am Mosq Control Assoc. 7: 210-237.
5. Tamarina NA (1975) Natural control of bloodsuckingmosquito population densities (review ofliterature). Med Parazitol (Mosk). 44: 603-607.
6. Ochoa SA, Sanchez-Torres LE, Nevarez-Moorillon GV, Camacho AD, Nogueda-Torres B(2017) Essential oils and their components as analternative in the control of mosquito vectors ofdisease. Biomedica. 37:224-243.
7. Fallatah SA, Khater EI (2010) Potential of medicinal plants in mosquito control. J Egypt Soc Parasitol. 40:1-26.
8. Park JH, Lee HS (2018) Toxicities of Eucalyptus dives oil, 3-Carvomenthenone, and its analoguesagainst stored-product insects. J Food Prot. 81:653-658.
9. Batish DR, PalSing H, Kumar Kohli R, Kaura S (2008) Eucalyptus essential oil as a natural pesticide. For Ecol Manag.256: 2166-2174.
10. Juan LW, Lucia A, Zerba EN, Harrand L, Marco M, Masuh HM (2011) Chemical composition and fumigant toxicity of the essential oils from 16species of Eucalyptus against Haematobia irritans (Diptera: Muscidae) adults. J Econ Entomol. 104: 1087-1092.
11. Mossi AJ, Astolfi V, Kubiak G, Lerin L, Zanella C, Toniazzo G, Oliveira D, Treichel H, Devilla IA, Cansian R, Restello R (2011) Insecticidal andrepellency activity of essential oil of Eucalyptussp. against Sitophilus zeamais Motschulsky
(Coleoptera, Curculionidae). J Sci FoodAgric.91: 273-277.
12. Alzogaray RA, Lucia A, Zerba EN, Masuh HM (2011) Insecticidal activity of essential oils from eleven Eucalyptus spp. and two hybrids: lethal and sublethal effects of their major componentson Blattella germanica. J Econ Entomol. 104:595-600.
13. Anjali CH, Sharma Y, Mukherjee A,Chandrasekaran N (2012) Neem oil (Azadirachtaindica) nanoemulsion--a potent larvicidal agentagainst Culex quinquefasciatus. Pest Manag Sci.68: 158-163.
14. Salvia-Trujillo L, Soliva-Fortuny R, Rojas-Grau MA, McClements DJ, Martin-Belloso O (2017) Edible Nanoemulsions as Carriers of Active Ingredients: A Review. Annu Rev Food Sci Technol. 8: 439-466.
15. Kumar M, Bishnoi RS, Shukla AK, Jain CP (2019)Techniques for formulation of nanoemulsion drugdelivery system: A review. Prev Nutr Food Sci.24: 225-234.
16. Sarheed O, Shouqair D, Ramesh K, Khaleel T, Amin M, Boateng J, Drechsler M (2020)Formation of stable nanoemulsions by ultrasoundassisted two-step emulsification process for topical drug delivery: Effect of oil phase composition and surfactant concentration and loratadine as ripening inhibitor. Int J Pharm. 576: 118952.
17. Wang, L, Mutch KJ, Eastoe J, Heenan RK, Dong J (2008) Nanoemulsions prepared by a two-step low-energy process. Langmuir. 24: 6092-6099.
18. Kumar S, Nehra M, Dilbaghi N, Marrazza G,Hassan AA, Kim KH (2019) Nano-based smart pesticide formulations: Emerging opportunities for agriculture. J Control Release, 294, 131-153.
19. World Health Organization (2005) Guidelines for laboratory and field testing of mosquito larvicides.WHO/CDS/WHOPES/GCDPP/2005.13. pp39
20. Finney DJ (1971) Probit Analysis. 3rd ed.Cambridge University press, London.pp 333
21. Azarian-Hamidi S, Harbach RE (2009). Keys to the adult females and fourth-instar larvae of the mosquitoes of Iran (Diptera: Culicidae). Zootaxa, 2078(1), 1–33.
22. Yassin M, Ton J, Rolfe SA, Valentine TA, Cromey M, Holden N, Newton AC (2021) The rise, fall and resurrection of chemical-induced resistanceagents. Pest Manag Sci. 10.1002/ps.6370
23. Gurusubramanian G, Rahman A, Sarmah M,Ray S, Bora S (2008) Pesticide usage pattern in tea ecosystem, their retrospects and alternativemeasures. J Environ Biol. 29: 813-826.
24. Casida JE (2016) Unexpected metabolic reactionsand secondary targets of pesticide action. J Food Chem. 64: 4471-4477.
25. Smith RF, Calvert DJ (1976) Health-related aspects of integrated pest management. Environ Health Perspect. 14: 185-191.
26. Jaoko V, Nji Tizi Taning C, Backx S, MulatyaJ, Van den Abeele J, Magomere T, Olubayo F, Mangelinckx S, Werbrouck SPO, Smagghe G (2020) The phytochemical composition of Melia volkensii and its potential for insect pest management. Plants (Basel). 9(2): 143-150.
27. Casida JE (2012) The greening of pesticideenvironment interactions: some personal observations. Environ Health Perspect. 120: 487-493.
28. Chaudhari AK, Singh VK, Kedia A, Das S, Dubey NK (2021) Essential oils and their bioactive compounds as eco-friendly novel green pesticides for management of storage insect pests: prospects and retrospects. Environ Sci Pollut Res Int. 28:18918-18940.
29. Luiz de Oliveira J, Ramos Campos EV, Fraceto LF (2018) Recent developments and challenges for nanoscale formulation of botanical pesticides for use in sustainable agriculture. J Agric Food Chem. 66: 8898-8913.
30. Tian Y, Dong F, Zhou X, Yang X (2020) Repellent,insecticidal and antimicrobial activities of leaf essential oils from three eucalyptus species. ChemBiodivers. 17:e1900580.
31. Magesa SM, Kamugisha ML (2006) Evaluation of the bio-efficacy of three brands of repellents against wild populations of anthropophilic mosquitoes. Tanzan Health Res Bull. 8: 145-148.
32. Cheng SS, Huang CG, Chen YJ, Yu JJ, Chen WJ,Chang ST (2009) Chemical compositions and larvicidal activities of leaf essential oils from two eucalyptus species. Bioresour Technol. 100: 452-456.
33. Senthil Nathan S (2007) The use of Eucalyptus tereticornis Sm. (Myrtaceae) oil (leaf extract) as a natural larvicidal agent against the malaria vector Anopheles stephensi Liston (Diptera: Culicidae).Bioresour Technol. 98: 1856-1860.
34. Kweka EJ, Mosha F, Lowassa A, Mahande AM,Kitau J, Matowo J, Mahande MJ, Massenga CP,Tenu F, Feston E. Lyatuu E, Mboya M, Mndeme R, Chuwa G, Temu E (2008) Ethnobotanical study of some of mosquito repellent plants in north-eastern Tanzania. Malar J. 7:152.
35. Perugini L, Cinelli G, Cofelice M, Ceglie A, Lopez F, Cuomo F (2018) Effect of the coexistence of sodium caseinate and Tween 20 as stabilizers of food emulsions at acidic pH. Colloids Surf B Biointerfaces.168: 163-168.
36. Gonzalez A, Gutierrez-Cutino M, Moenne A (2014) Oligo-carrageenan kappa-induced reducing redox status and increase in TRR/TRX activities promote activation and reprogramming of terpenoid metabolism in Eucalyptus trees.Molecules.19: 7356-7367.
| Files | ||
| Issue | Vol 15 No 4 (2021) | |
| Section | Original Article | |
| DOI | https://doi.org/10.18502/jad.v15i4.10502 | |
| Keywords | ||
| Eucalyptus Essential oil Nanoemulsion Larvicide Anopheles stephensi Culex pipiens | ||
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Sabzalizade S, Amani A, Vatandoost H, Hosseini F, Najafi-Taher R, Basseri HR. Evaluation of Nanoemulsion of Eucalyptus globulus Oil as Potent Botanical Larvicide Against Malaria Vector , Anopheles stephensi and West Nile Vector, Culex pipiens Under Laboratory and Semi-field Conditions. J Arthropod Borne Dis. 2022;15(4):380-388.
